Enzymatic unmasking for antibodies of penicilloyl residues bound to albumin

Wal, Jean‐Michel

doi:10.1016/0006-2952(80)90328-7

Cited by 16 publications

(3 citation statements)

References 9 publications

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“…The majority of these adverse reactions are believed to have an immunological etiology [3], Based on the hypothesis that drug hypersensitivity arises when drugs, which are low-molecular compounds, become immunogenic after binding irreversibly with mac romolecules [4,5] such as tissue or serum proteins or after polymerization, it is considered that the immune system recognizes drug-protein conjugates and produces drugspecific antibodies [6]. Penicillins have generally been em ployed as a model for drug hypersensitivity in animal sys tems and the major antigenic determinant derived from penicillin has been identified as a protein-conjugated penicilloyl group [7][8][9], In recent years, many cephalosporin ABs have been developed and clinically used. Cephalosporins also con tain ß-lactam rings, and allergic responses have been ob served after cephalosporin therapy.…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity of Various Beta-Lactam Antibiotic-Protein Conjugates and Cross-Reactivity of the Antibodies Produced in Guinea Pig

Katsutani¹,

Shionoya²

1993

Int Arch Allergy Immunol

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Immunogenic properties of bovine serum albumin (BSA) conjugates of various β-lactam antibiotics (ABs) and cross-reactivity of the produced antibodies were investigated in guinea pigs. Each AB-BSA conjugate was immunized with complete Freund’s adjuvant. Following the second injection, AB-specific antibodies were detected with high titers. Produced antibodies showed concentration-dependent inhibition by the corresponding AB. Cross-reactivity between the produced antibodies and ABs was evaluated with two methods, i.e. by testing for reactivity with the various AB-ovalbumin conjugates, and by assaying for inhibition of enzyme-linked immunosorbent assay (ELISA) by the various ABs. The latter method could detect minor cross-reactions more sensitively than the former one. The results suggested that similarity of the acyl side chain of a cephalosporin nucleus or a penicillin nucleus contributes to immunological cross-reactivity.

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Section: Introductionmentioning

confidence: 99%

Immunogenicity of Various Beta-Lactam Antibiotic-Protein Conjugates and Cross-Reactivity of the Antibodies Produced in Guinea Pig

Katsutani¹,

Shionoya²

1993

Int Arch Allergy Immunol

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“…It is known that the degree of penicilloylation achieved in vivo by penicillin therapy is low. In a experimental study by Wal (1980), for example, in which a very high intramuscular dose of benzylpenicillin (6g) was administered to a pig, covalently-bound penicilloyl residues could be detected at a peak level of little more than 10 ng/mg albumin, representing 0.002 penicilloyl groups per protein molecule.…”

Section: Primary Sensitizationmentioning

confidence: 99%

“…In a different context, that is following systemic treatment of pigs with benzylpenicillin, Wal (1980) has shown that penicilloylation of porcine serum albumin can occur and that these modified albumins are eliminated by two routes. Rapid elimination follows the interaction of conjugate with antibody to the penicilloyl group, but Wal claims that some penicilloyl groups may be masked inside the tertiary structure of the protein and are thus unavailable to antibody interaction.…”

Section: Reactions In Sensitized Individualsmentioning

confidence: 99%