“…Aliquots of the reaction mixture were detached at regular intervals and subjected to UV-visible spectrophotometric measurements performed on a Shimadzu spectrophotometer (model UV-1601) worked at a resolution of 1 nm. After completing of the reaction, gold nanoparticles were collected by centrifugation (8500×g, 30 min) and washed twice with distilled water, and the unbound proteins were removed by treating with 80 % (v/v) ethanol [23,33].…”
Section: Bioproduction and Characterization Of Gold Nanoparticlesmentioning
confidence: 99%
“…The biological reduction of gold ions in aqueous solution was checked by periodic sampling of aliquots (1 ml) and then measuring UV-Vis spectra of the solution. UV-Vis spectra of the enzyme aliquots were checked as a function of time of reaction on UV-Vis spectrophotometer UV-1601 (Shimadzu, Japan) [33].…”
“…The viscosity, refractive index and absorption values were providing in the Malvern software for water (µ = 0.8872 cP, RI = 0.135). Ten runs were averaged for each watery sample for accuracy of determination [33].…”
“…The samples were left to dry completely at room temperature. SEM measurements were performed on a FESEM 50VP, Leo SUPRA instrument [33].…”
Section: Scanning Electron Microscopy (Sem)mentioning
confidence: 99%
“…The gold nanoparticles were placed on the carbon-coated copper grids and TEM analysis was performed using a Philips model EM 208S operating at 80 kV [33].…”
Section: Transmission Electron Microscopy (Tem)mentioning
by disk diffusion method indicated a potent growth inhibitory activity against all tested human pathogenic yeasts and molds by inhibition zones ranged from 10 to 18 mm. Taken together, our enzymatically established method of nanoparticle synthesis using a purified sulfite oxidoreductase of F. oxysporum can be considered as an efficient tool for generating harmless bioactive gold nanoparticles with potential applications in biology, medicine and industry.
“…Aliquots of the reaction mixture were detached at regular intervals and subjected to UV-visible spectrophotometric measurements performed on a Shimadzu spectrophotometer (model UV-1601) worked at a resolution of 1 nm. After completing of the reaction, gold nanoparticles were collected by centrifugation (8500×g, 30 min) and washed twice with distilled water, and the unbound proteins were removed by treating with 80 % (v/v) ethanol [23,33].…”
Section: Bioproduction and Characterization Of Gold Nanoparticlesmentioning
confidence: 99%
“…The biological reduction of gold ions in aqueous solution was checked by periodic sampling of aliquots (1 ml) and then measuring UV-Vis spectra of the solution. UV-Vis spectra of the enzyme aliquots were checked as a function of time of reaction on UV-Vis spectrophotometer UV-1601 (Shimadzu, Japan) [33].…”
“…The viscosity, refractive index and absorption values were providing in the Malvern software for water (µ = 0.8872 cP, RI = 0.135). Ten runs were averaged for each watery sample for accuracy of determination [33].…”
“…The samples were left to dry completely at room temperature. SEM measurements were performed on a FESEM 50VP, Leo SUPRA instrument [33].…”
Section: Scanning Electron Microscopy (Sem)mentioning
confidence: 99%
“…The gold nanoparticles were placed on the carbon-coated copper grids and TEM analysis was performed using a Philips model EM 208S operating at 80 kV [33].…”
Section: Transmission Electron Microscopy (Tem)mentioning
by disk diffusion method indicated a potent growth inhibitory activity against all tested human pathogenic yeasts and molds by inhibition zones ranged from 10 to 18 mm. Taken together, our enzymatically established method of nanoparticle synthesis using a purified sulfite oxidoreductase of F. oxysporum can be considered as an efficient tool for generating harmless bioactive gold nanoparticles with potential applications in biology, medicine and industry.
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