Enzymatic synthesis of gold nanoparticles using sulfite reductase purified from Escherichia coli: A green eco-friendly approach

Bioinspired synthesis, characterization and antifungal activity of enzyme-mediated gold nanoparticles using a fungal oxidoreductase

“…Aliquots of the reaction mixture were detached at regular intervals and subjected to UV-visible spectrophotometric measurements performed on a Shimadzu spectrophotometer (model UV-1601) worked at a resolution of 1 nm. After completing of the reaction, gold nanoparticles were collected by centrifugation (8500×g, 30 min) and washed twice with distilled water, and the unbound proteins were removed by treating with 80 % (v/v) ethanol [23,33].…”

“…The biological reduction of gold ions in aqueous solution was checked by periodic sampling of aliquots (1 ml) and then measuring UV-Vis spectra of the solution. UV-Vis spectra of the enzyme aliquots were checked as a function of time of reaction on UV-Vis spectrophotometer UV-1601 (Shimadzu, Japan) [33].…”

“…The viscosity, refractive index and absorption values were providing in the Malvern software for water (µ = 0.8872 cP, RI = 0.135). Ten runs were averaged for each watery sample for accuracy of determination [33].…”

“…The samples were left to dry completely at room temperature. SEM measurements were performed on a FESEM 50VP, Leo SUPRA instrument [33].…”

“…The gold nanoparticles were placed on the carbon-coated copper grids and TEM analysis was performed using a Philips model EM 208S operating at 80 kV [33].…”