Enzymatic Synthesis of Butyl Ferulate by Silica-Immobilized Lipase in a Non-Aqueous Medium

Abstract

Butyl ferulate was synthesized using a silica-immobilized commercial lipase (Steapsin) in dimethylsulfoxide (DMSO

Immobilization of Lipase from <i>Geobacillus</i> sp. and Its Application in Synthesis of Methyl Salicylate

“…5 . In a previous study, silica bound commercial lipase, Steapsin showed a moderate increase in the synthesis of isopropyl ferulate with 100 mg/mL of molecular sieves in a 2 mL of reaction mixture 38 . It appears that an excess concentration of molecular sieves completely removed even traces of water molecules from the reaction mixture, thus rendering the bound-lipase relatively ineffective to perform the esterification reaction which resulted in a gradual decline in the ester yield.…”

“…Frequent encountering of substrate into the active site might have caused its distortion, thus reducing its catalytic efficiency either partially or fully. In previous studies, the synthesis of isopropyl ferulate and butyl ferulate by silica bound commercial lipase, Steapsin was achieved in short periods of 3 h, and 6 h, respectively 37,38 . In these studies, a good yield of the respective ester s was noted in the 1st and 2nd cycles of esterification/reuse but, ultimately, the product yield continued to decline with successive cycles of reuse.…”

“…In the synthesis of isopropyl ferulate, the silica bound commercial lipase, Steapsin gave a poor yield of 14.8 mM at 45 after six cycles of esterification 38 . In another study, Candida rugosa lipase immobilized on glass beads retained 78 and 85 of its initial activity after ten hydrolytic cycles in a batch system 39 .…”

“…In another study, Candida rugosa lipase immobilized on glass beads retained 78 and 85 of its initial activity after ten hydrolytic cycles in a batch system 39 . Previously, commercial lipase Lipolase T100 was immobilized on to silica and after seven cycles the yield of linalyl ferulate ester was found to be 53.9 38 . Fig.…”

Synthesis and Characterization of Phenolic Lipids

“…Any water that reaches the enzyme for participation in the reaction must difuse from the bulk emulsion. Thus, to avoid difusional limitations, the oil phase must be well saturated with water [105].…”