Enzymatic surface treatment of poly (3‐hydroxybutyrate) (PHB), and poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV)

Abstract

BACKGROUND: Polyhydroxyalkanoates (PHA) are very interesting biopolymers, their most promising applications lie in biomedical science. The wettability of the surface of PHA increases their biocompatibility properties, making them suitable for such applications. RESULTS: The treatment of the surface of poly (3-hydroxybutyrate) (PHB) and poly (3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV) films with the cutinase TfCut2 from Thermobifida fusca KW3 resulted in decrease of the water contact angle of PHB films from 90 ∘ to 36 ∘ and PHBV films from 93-98 ∘ to 50-60 ∘ with 3-hydroxyvalerate content ranging from 6 to 9 mol%, respectively. CONCLUSION: A linear dependence up to 9 mol% was found between the 3-hydroxyvalerate content of the PHBV and the resulting water contact angle decrease, caused by partial hydrolysis of the surface of the films by the cutinase. PHBV films with a 3-hydroxyvalerate content of 6 mol% showed the highest gain in surface hydophilicity following treatment with the enzyme. Preparation of PHA filmsThe films were prepared by a solvent casting technique. A solution of 10 g L −1 of PHA in chloroform was prepared and filtered to remove insoluble parts. 30 g of the solution were poured into Figure 2. SEM of PHB films. (A) untreated; (B), (C) treated with TfCut2 cutinase for 2 h; (D) treated with TfCut2 cutinase for 24 h.

Biodegradation of diethyl terephthalate and polyethylene terephthalate by a novel identified degraderDelftiasp. WL-3 and its proposed metabolic pathway

“…YP1, which could cause obvious damage to PE film, including pits and cavities on the surface. Berezina et al (2015) reported that PHB film treated with the cutinase TfCut2 showed a rough surface compared to the untreated control, suggesting a degradation of the outermost surface layers of the film. In this study, although the degradation of PET by strain WL-3 was observed using SEM, the degradation ability was not determined quantitatively.…”

Improvement of the poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production by dual feeding with levulinic acid and sodium propionate in Cupriavidus necator

“…However, certain applications require less brittle, more elastic polymer with lower glass transition temperature (T g ). This can be achieved by modulation of PHB composition and the incorporation of the 3-hydroxyvalerate (3-HV) monomeric units [16,17].…”