Improvement of the poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production by dual feeding with levulinic acid and sodium propionate in Cupriavidus necator

Abstract

In the context of increasing volatility of oil prices, replacement of petroleum based plastics by bioplastics is a topic of increasing interest. Poly(hydroxyalkanoate)s (PHAs) are among the most promising families in this field. Controlling composition of the polymer on the monomeric level remains a pivotal issue. This control is even more difficult to achieve when the polymer is not synthesized by chemists, but produced by nature, in this case, bacteria. In this study mechanism and role of two 3-hydroxyvalerate (3-HV) inducing substrates on the production of PHBV with high, 80%, 3-HV content were evaluated. It was found that levulinic acid contributes to biomass and bio-polymer content enhancement, whereas sodium propionate mainly contributes to 3-HV enhancement. Optimized proportions of feeding substrates at 1 g/L and 2.5 g/L, respectively for levulinic acid and sodium propionate allowed a 100% productivity enhancement, at 3.9 mg/L/hour, for the production of PHBV with 80% 3-HV.

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with l-isoleucine in Corynebacterium glutamicum WM001

“…As 2-ketobutyrate is one of the crucial intermediates in l -isoleucine synthesis, there might be excessive 2-ketobutyrate in WM001, and could be channeled towards propionyl-CoA synthesis; (IV) the (2R)-methylmalonyl-CoA pathway, in which succinyl-CoA could convert to propionyl-CoA through sbm and ygfG genes [ 54 ], but this coenzyme vitamin B 12 dependent pathway does not exist in C. glutamicum ; (V) the levulinyl-CoA pathway, which was identified in C. necator . The acetyl-CoA and propionyl-CoA could be converted from levulinate [ 55 ] and used for PHBV production in C. necator [ 56 ]. (VI) other pathways, in Rhodococcus aetherivorans , PHBV could be produced with toluene or acetate but the mechanism remains obscure [ 47 ].…”

“… Glucose, levulinic acid 76.5 4.03 [ 60 ] R. eutropha Glucose, valerate 62.7 110.2 [ 17 ] R. aetherivorans Acetate 79 2.5 [ 47 ] H. mediterranei Starch 13.4 7.01 [ 61 ] C. glutamicum Glucose, propionate 28 12.0 [ 22 ] E. coli Glucose 25.4 – [ 51 ] R. eutropha Glucose 26.0 132.8 [ 62 ] Bacillus spp. Glucose, l -threonine 48 2.02 [ 19 ] C. necator Glucose, levulinic acid, sodium propionate 80 – [ 56 ] E. coli Glucose, propionate 67.9 2.9 [ 16 ] C. glutamicum Glucose 75.2 76 …”

A Critical Review on the Economically Feasible and Sustainable Poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) Production from Alkyl Alcohols

“…Bacteria capable of using levulinic acid as the 3HV precursor are rare and are mainly C. necator, with the exception of Burkholderis sp. IS-01 and Hydrogenophaga pseudoflava DSM 1034 [10,11,[65][66][67][68]. C. necator KHB-8862 and H. pseudoflava DSM 1034 are two promising strains reported with a high 3HV yield of 0.50 and 1.00 g/g, respectively.…”

Influence of the total concentration and the profile of volatile fatty acids on polyhydroxyalkanoates (PHA) production by mixed microbial cultures

“…was the most common genus in the inoculum, followed by Corynebacterium (n = 5) and Enterobacter (n = 4), while Salana, Pseudoclavibacter, Agrococcus, Stenotrophomonas, and Microbacterium were identified in one isolate. Of these, the genera Enterobacter [32], Corynebacterium [33], Cupriavidus [34,35], and Microbacterium [36,37] have been previously described as PHA producers. In addition, Cupriavidus necator is the most widely used Gram-negative bacterium due to its high PHA production capacity [8].…”