Enzymatic sensor for phenols based on titanium dioxide generating surface confined ROS after treatment with H2O2

Fundamentals, Applications, and Future Directions of Bioelectrocatalysis

“…Rahemi et al investigated the use of TiO 2 to generate reactive oxygen species (ROS) in the presence of hydrogen peroxide (H 2 O 2 ). 539 The researchers reported an original method with a short preactivation step of TiO 2horseradish peroxidase (HRP) to design an HRP-based biosensor that works in solutions without H 2 O 2 . As such, this EEB works as a reagent-less biosensor that can detect phenols, as well as aromatic amines, without the need to add cosubstrates in the measuring solutions.…”

“…Since the same enzyme can be used for different analyte detection, a precise calibration curve of a mixture is needed to develop a practical EEB based on tyrosinase. Rahemi et al investigated the use of TiO 2 to generate reactive oxygen species (ROS) in the presence of hydrogen peroxide (H 2 O 2 ) . The researchers reported an original method with a short preactivation step of TiO 2 -horseradish peroxidase (HRP) to design an HRP-based biosensor that works in solutions without H 2 O 2 .…”

An iridium‐based phosphorescence probe for HClO/ClO− detection and application of recognizing exogenous/endogenous HClO/ClO− in cells

“…7,8 Due to its strong oxidation, hypochlorous acid is not only used as disinfection and bleaching in daily environment 9 but also plays a key role in various biological processes. [10][11][12][13][14] For example, hypochlorous acid can act as a powerful antibacterial agent in living organisms against bacteria, viruses and other substances that threaten the immune system. [15][16][17][18] However, it is also a double-edged sword, abnormal hypochlorous acid levels in mitochondria can cause mitochondrial dysfunction.…”

“…As an important member of highly reactive ROS, 5,6 endogenous hypochlorous acid (HClO) is a peroxide derivative formed under the catalysis of myeloperoxidase and is usually found mainly in mitochondria 7,8 . Due to its strong oxidation, hypochlorous acid is not only used as disinfection and bleaching in daily environment 9 but also plays a key role in various biological processes 10–14 . For example, hypochlorous acid can act as a powerful antibacterial agent in living organisms against bacteria, viruses and other substances that threaten the immune system 15–18 .…”

Development of a combi-electrosensor for the detection of phenol by combining photoelectrochemistry and square wave voltammetry

“…28 On the other hand, the modification of electrodes by enzymes promotes an improvement of the sensor's sensitivity with detection limits (LOD) in the µmol L −1 to nmol L −1 range. 39 Enzymes such as horseradish peroxidase (HRP), [40][41][42] tyrosinase 43,44 and laccase 39 are often employed to accomplish a better sensitivity. Yet, thermal and chemical instability of the enzyme, reproducibility issues and the use of reagents, such as hydrogen peroxide (H2O2) in the case of HRP, limit their applicability in real scenarios.…”