Enzymatic saccharification of spent wood-meal media made of 5 different tree species after cultivation of edible mushroom Auricularia polytricha

Abstract

The chemical characteristics and the suitability for enzymatic saccharification in the spent culture media (SCM) of Auricularia polytricha were examined in order to investigate the utilization of the SCM as a biomass resource for alternative energy production. Wood meals from 3 hardwood species (Quercus serrata, Betula platyphylla var. japonica, and Alnus japonica) and 2 softwood species (Pinus densiflora and Cryptomeria japonica) were used as basal culture media. Dry weight of fruiting bodies were higher in the cultural media made of B. platphylla var. japonica and A. japonica. Amount of weight loss in media showed almost the same value among the cultural media made of 5 species, except for media made of C. japonica. The amounts of the main chemical components (Klason lignin, holocellulose, and a-cellulose) in SCM showed lower values than those in wood meals (WM) and fresh media (FM). After saccharification of the media by Meiselase for 48 h, the hydrolysis weight decrease dramatically increased in SCM. The amount of glucose in SCM ranged from 10.9 to 19.2 g/100 g dry biomass. The highest amount of glucose was obtained in the SCM from B. platyphylla var. japonica. These results indicate that SCM of A. polytricha is a suitable biomass material to produce fermentable sugars for bio-ethanol production.

Ozone oxidation pretreatment for enzymatic saccharification of spent culture media after Lentinula edodes cultivation

“…White-rot fungi can degrade wood components such as lignin, cellulose and hemicellulose [8]. It has been reported that the lignin content of SCM after mushroom cultivation is lower than that of fresh media and wood meal [9] and for this reason, SCM is considered a suitable raw material for ethanol production by enzymatic saccharification. Use of SCMs as raw material for ethanol production has been investigated by several researchers [5,6,10].…”

Comparison of glucose yield from rubberwood sawdust (RSD), growth medium (GM), and mushroom spent medium (MSM) under different sodium hydroxide pretreatment techniques

“…The amount of lignin and holocellulose in both woodmeal were lower compared to spent culture medium. Thus, indicated that apart of lignin, this mushroom type also degraded cellulose and hemicelluloses during the cultivation [12].…”

Association of Mushroom Cultivation and Ozonolysis as Pretreatment for Enzymatic Saccharification of Sengon(Falcataria moluccana) Sawdust

“…In terms of pre-treating lignocellulose for subsequent enzymatic saccharification, the advantage of ozone treatment is highly selective to removal of lignin with slight degradation of hemicelluloses, whereas it hardly affects cellulose (Sun & Cheng 2002). Mushroom cultivation media waste has a lower lignin content because the content of lignin was reduced during the growth of fungi (Irawati et al 2012a). In this study, we observed the optimal duration of ozone treatment for sengon wood waste and sengon that was used for mushroom cultivation (spent media).…”