Enzymatic Recovery of Metabolites from Seaweeds

Hardouin, Kévin; Bedoux, Gilles; Burlot, Anne-Sophie; Nyvall-Collén, Pi; Bourgougnon, Nathalie

doi:10.1016/b978-0-12-408062-1.00010-x

Cited by 46 publications

(27 citation statements)

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“…According to the present results, the obtained extracts were free from the cytotoxic effects toward Caco-2 and therefore may have importance as a food component [43]. Algal extracts obtained using the EAE method were also not toxic toward other epithelial cell lines, e.g., VERO [11,44]. Typically, the extracts prepared using standard methods, e.g., organic or water, are cytotoxic at a very low concentration of algal biomass and are considered as potential anticancer agents [45,46].…”

Section: Resultsmentioning

confidence: 89%

“…Typically, the extracts prepared using standard methods, e.g., organic or water, are cytotoxic at a very low concentration of algal biomass and are considered as potential anticancer agents [45,46]. In turn, the algal extracts obtained using the EAE method are well-tolerated by mammalian cells even at high concentrations [11,44]. However, the mechanism of the cytotoxic effect of extracts observed after 24 h of Caco-2 propagation should be further examined, because it may also be associated with the bioavailability of compounds derived from obtained extracts, as well as with their absorption kinetics [47].…”

Section: Resultsmentioning

confidence: 99%

“…Liang et al (2012) indicated that the immobilization of enzymes can make them more economical since it facilitates the separation of enzyme from the product and its reuse [10]. In EAE, common enzymes that are used in the food industry (e.g., neutral and alkaline protease, α-amylase, cellulase and pepsin) can be used [3,8,11]. They are obtained by the fermentation of bacteria or fungi [11].…”

Section: Introductionmentioning

confidence: 99%

“…In EAE, common enzymes that are used in the food industry (e.g., neutral and alkaline protease, α-amylase, cellulase and pepsin) can be used [3,8,11]. They are obtained by the fermentation of bacteria or fungi [11]. …”

Section: Introductionmentioning

confidence: 99%

“…This method is mainly used for the isolation of polysaccharides, phenols and pigments due to their water-soluble character [12,13]. The extracted compounds (especially polysaccharides and phenols) are known to possess antibacterial, antioxidative, anti-inflammatory, antitumor and antiviral properties [3,11,13]. …”

Section: Introductionmentioning

confidence: 99%

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Chemical Characterization of Enteromorpha prolifera Extract Obtained by Enzyme-Assisted Extraction and Its Influence on the Metabolic Activity of Caco-2

Michalak

Dmytryk

Śmieszek

et al. 2017

IJMS

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The green seaweed Enteromorpha prolifera was used as a feedstock for the production of enzymatic hydrolysate using cellulase. The selection of the conditions for enzymatic hydrolysis of the biomass was carried out for different enzyme doses and incubation periods. The obtained extract was examined in terms of its multielemental composition, content of polyphenols and antibacterial properties (tested against Escherichia coli and Staphylococcus aureus). Additionally, its influence on the metabolic activity of human colon epithelial cells (Caco-2) was analyzed. The tested concentrations of extract using an in vitro model were 62.5, 125, 250, 500, 1000 and 2000 µg/mL. The hydrolysis yield in the most suitable experimental conditions (8-h process and 50 and 100 µL of cellulase) was 36%. Micro- and macroelements were poorly extracted from the algal biomass. Total phenolic content was 55 mg of gallic acid equivalent per 100 g of dry mass of extract. The cytotoxic effect of extracts, related to the inhibition of the metabolic activity of Caco-2, was noted only after 24 h. In turn, cultures of Caco-2 propagated with extracts for 72 h were characterized by significantly elevated metabolism (the concentration of extracts ranged from 62.5 to 1000 µg/mL, p < 0.05). Obtained results indicated the high biological activity of the prepared extracts; however, the observed effects did not occur in a dose-dependent manner.

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Section: Resultsmentioning

confidence: 89%