Enzymatic Production of Monoacylglycerols (MAG) and Diacylglycerols (DAG) from Fish Oil in a Solvent‐Free System

Feltes, Maria Manuela Camino; Villeneuve, Pierre; Baréa, Bruno; Barouh, Nathalie; Oliveira, J. Vladimir; Oliveira, Débora de; Ninow, Jorge Luiz

doi:10.1007/s11746-011-1998-2

Cited by 38 publications

(18 citation statements)

References 32 publications

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“…When the substrate ratio was increased from 1:1 to 1:3 mol/mol, the DAG content did not change appreciably after 12 hours of reaction, and the final DAG content decreased as the substrate ratio increased, likely due to increased alcoholysis of DAG by glycerol to form MAG. The results are in agreement with the study from Feltes et al (), who formed a product with 41% of DAG content through Novozym® 435‐catalyzed glycerolysis of fish oil at an oil/glycerol molar ratio of 1:1. However, in the cited paper, the content of MAG was 25%, which was higher than that of this experiment, likely due to the higher reaction temperature (70 °C) and longer reaction time (24 hours) used in the cited study.…”

Section: Resultssupporting

confidence: 92%

Preparation of Docosahexaenoic Acid‐Rich Diacylglycerol‐Rich Oil by Lipase‐Catalyzed Glycerolysis of Microbial Oil from Schizochytrium sp. in a Solvent‐Free System

Zou¹,

Nadege²,

Ninette³

et al. 2019

J Americ Oil Chem Soc

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Docosahexaenoic acid (DHA)‐rich diacylglycerol (DAG)‐rich oil was prepared by lipase‐catalyzed glycerolysis of microbial oil from Schizochytrium sp. in a solvent‐free system. The reaction parameters including lipase type, substrate molar ratio, temperature, lipase concentration, and reaction time were screened. The selected conditions were determined as follows: Novozym® 435 (Novozymes A/S, Bagsvaerd, Denmark) as biocatalyst at 8 wt%, substrate ratio (DHA‐rich microbial TAG/glycerol) of 1:1 mol/mol, temperature of 50 °C, and reaction time of 12 hours. Under these conditions, the triacylglycerol (TAG), DAG, and monoacylglycerol (MAG) contents in the product were 36.4%, 48.2%, and 15.4%, respectively. The lipase was reused successively for 18 cycles without significant loss of activity under the conditions given above. Fatty acid composition analysis of the final product showed that the contents of DHA in TAG, DAG, and MAG were 53.9%, 44.9%, and 34.8%, respectively. DHA‐rich DAG has the potential to be used as an ingredient in infant formula to increase the bioavailability of DHA.

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Section: Resultssupporting

confidence: 92%

Preparation of Docosahexaenoic Acid‐Rich Diacylglycerol‐Rich Oil by Lipase‐Catalyzed Glycerolysis of Microbial Oil from Schizochytrium sp. in a Solvent‐Free System

Zou¹,

Nadege²,

Ninette³

et al. 2019

J Americ Oil Chem Soc

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“…The production of partial glycerides esterified with fatty acids of bioactive interest is also now possible (Devi et al, 2008;Feltes et al, 2012;Vadivel, Whitten, & Makriyannis, 2011;Wang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Comparative in vitro intestinal digestion of 1,3-diglyceride and 1-monoglyceride rich oils and their mixtures

Martín

Morán-Valero

Vázquez

et al. 2014

Food Research International

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“…Enzymatic catalysis in solvent-free medium (SFM) has atracted considerable interest in the recent years [90]. It used as an eicient approach to the synthesis of natural products, pharmaceuticals and food ingredients.…”

Section: Enzyme Reactions In Solvent-free Medium (Sfm)mentioning

confidence: 99%

Synthesis and Characterization of Phenolic Lipids

Roby¹

2017

Phenolic Compounds - Natural Sources, Importance and Applications

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Omega-3 polyunsaturated faty acids (ω3 PUFAs) from ish oils promote well-established health and antiaging beneits that justify their use as functional ingredients in dietary supplements, healthy foods, and nutraceutical products. Dietary supply is needed because human metabolism exhibits limited to synthesize ω3 PUFAs. However, the practical use of such lipids as food ingredients is often limited due to their high susceptibility to oxidation, which is responsible for the undesirable of-lavor and odor of rancid oils, associated with the loss of nutritional value. Produced phenolic lipids were a good solution for all these problems. These phenolic lipids are preferentially produced by enzymatic bioprocesses that exhibit high selectivity toward polyfunctional substrates and mild reaction conditions compared with chemical synthesis pathways. This chapter presents the acylation of phenolic compounds and lipids using enzyme under various operating conditions. In conclusion, the acylation of lipids with natural phenolic compounds resulted in the formation of a lipophilic ester that should be able to stabilize the oil, fats, and emulsions against oxidation. Acylation of lipids to phenolic compounds that have antioxidant properties thus protects the lipids from oxidation, and the phenolic lipid derivatives carry the combined health beneicial properties of lipids and the phenolic molecules.

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Enzymatic Production of Monoacylglycerols (MAG) and Diacylglycerols (DAG) from Fish Oil in a Solvent‐Free System

Cited by 38 publications

References 32 publications

Preparation of Docosahexaenoic Acid‐Rich Diacylglycerol‐Rich Oil by Lipase‐Catalyzed Glycerolysis of Microbial Oil from Schizochytrium sp. in a Solvent‐Free System

Preparation of Docosahexaenoic Acid‐Rich Diacylglycerol‐Rich Oil by Lipase‐Catalyzed Glycerolysis of Microbial Oil from Schizochytrium sp. in a Solvent‐Free System

Comparative in vitro intestinal digestion of 1,3-diglyceride and 1-monoglyceride rich oils and their mixtures

Synthesis and Characterization of Phenolic Lipids

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