Enzymatic production of human milk fat substitutes containing palmitic and docosahexaenoic acids at sn-2 position and oleic acid at sn-1,3 positions

Biotechnological and Novel Approaches for Designing Structured Lipids Intended for Infant Nutrition

“…It was stated that the product can be used in infant formulas [81]. In another study, Robles et al [85] increased the palmitic acid and DHA content at the sn-2 position and oleic acid at the sn-1,3 position of their substrate, tuna oil, via a four-step process, using Novozym 435 and consequently sn-1,3-specific lipase DF from Rhizopus oryzae as biocatalysts. These four steps can be summarized as acidolysis of tuna oil to synthesize structured TAGs (SLs) rich in PA and DHA at the sn-2 position, purification of TAGs, obtaining HMFS structure of OPO and ODO.…”

Effects of triacylglycerol structure and solid fat content on fasting responses of mice

“…POP and synthetic PPO were used to study the effect of palmitic acid positional distribution on lipid metabolism monitored as fasting responses, since POP and PPO have similar solid fat content at body temperature [28]. Synthetic POO and a fat mixture as described below with the same fatty acid and positional composition as a whole were used to study the effect of solid fat content.…”

Maximizing content of Omega-3 (EPA and DHA) in the process of enzymatic acidolysis of canola oil and concentrated of long-chain polyunsaturated fatty acids (LCPUFA) in supercritical CO2 conditionsDoes fruit cooling and seed film coating affect the germination potential of physalis?

“…Each of these lipid components were identified by TLC on silica gel plates (TLC Silica gel 60 -Merck Millipore). The mobile phase used was chloroform / acetone / methanol (95:4.5:0.5 v/v/v) (Robles et al, 2011). Four µL of sample was seeded (previously, 1:25 sample was diluted in hexane) and allowed to elute the solvent together with the analytes.…”