Enzymatic preparation and characterization of soybean lecithin-based emulsifiers

Jala, Ram Chandra Reddy; Chen, B.; Li, H.; Zhang, Y.; Cheong, Ling‐Zhi; Yang, Tiankui; Xu, Ximing

doi:10.3989/gya.0571161

Cited by 3 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of a second, different emulsifier would have brought with it the risk that displacement effects on the solid-liquid and liquid-liquid interfaces would occur [19]. However, with a hydrophobic-lipophilic balance (HLB) of 6.5-8.0 [30], lysolecithin was classified as a w/o emulsifier rather than an o/w emulsifier. Therefore, the stability of the resulting suspoemulsion was not expected to be sufficiently high.…”

Section: Production Of Suspoemulsionsmentioning

confidence: 99%

Production of Hydrogel-Based Curcumin-Loaded O/W Suspoemulsions

Bodmer

Hartmann²,

Keck³

et al. 2023

Future Pharmacology

View full text Add to dashboard Cite

Curcumin is a biopharmaceutical classification system (BCS) class IV substance with many potential therapeutic effects. However, like many other BCS IV active pharmaceutical ingredients, complex formulations are needed to guarantee a sufficiently high bioavailability. A not-so-well-known delivery system is a suspoemulsion (SE). SEs are emulsions with a crystalline API in continuous or dispersed phases. This study aimed to produce curcumin-loaded o/w suspoemulsions with the particle in the oil phase for, e.g., encapsulation or triggered release effects. The particles need to be smaller than the emulsion droplet size to attain high encapsulation efficiencies (EE) in the oil phase. Sonofragmentation and bead milling were tested for their ability to produce these nanocrystals in different dispersion media. It was discovered that production in miglyol was the best fit for the needed application of the crystals in SEs. Around 85% (by volume) of the particles produced with bead milling were smaller than the droplet size of about 5 µm. In contrast, only 23% of the sonofragmentated particles were below the diameter of those droplets. This oily suspension was then used to successfully produce hydrogel-based o/w suspoemulsions. In the second part of this study, we investigated different methods for determining encapsulation efficiency, but none of the methods accurately and satisfactorily resolved the encapsulation efficiency. Finally, the suspoemulsions could not be macroscopically distinguished from one another and were physically stable. In summary, we showed that stable hydrogel-based curcumin-loaded o/w suspoemulsions could be produced.

show abstract

Section: Production Of Suspoemulsionsmentioning

confidence: 99%

Production of Hydrogel-Based Curcumin-Loaded O/W Suspoemulsions

Bodmer

Hartmann²,

Keck³

et al. 2023

Future Pharmacology

View full text Add to dashboard Cite

show abstract

“…Lecithins are composed of 40% neutral lipids (36.1% triacylglycerol and 3.9% as mono‐. and diacylglycerols) and 60% PLS, of which 7% are lysophospholipids (LyPLS) but also phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) and phosphatidic acid (PA) .…”

Section: Technological Propertiesmentioning

confidence: 99%

Suitable simple and fast methods for selective isolation of phospholipids as a tool for their analysis

et al. 2018

View full text Add to dashboard Cite

Lipids are gaining relevance over the last 20 years, as our knowledge about their role has changed from merely energy/structural molecules to compounds also involved in several biological processes. This led to the creation in 2003 of a new emerging research field: lipidomics. In particular the phospholipids have pharmacological/food applications, participate in cell signalling/homeostatic pathways while their analysis faces some challenges. Their fractionation/purification is, in fact, especially difficult, as they are amphiphilic compounds. Moreover, it usually involves SPE or TLC procedures requiring specific materials hampering their suitableness for routine analysis. Finally, they can interfere with the ionization of other molecules during mass spectrometry analysis. Thus, simple high-throughput reliable methods to selectively isolate these compounds based on the difference between chemical characteristics of lipids would represent valuable tools for their study besides that of other compounds. The current review work aims to describe the state-of-the-art related to the extraction of phospholipids using liquid-liquid methods for their targeted isolation. The technological and biological importance of these compounds and ion suppression phenomena are also reviewed. Methods by precipitation with acetone or isolation using methanol seem to be suitable for selective isolation of phospholipids in both biological and food samples.

show abstract

Comparative study of modified soy lecithins as oil in water (O/W) emulsifiers.

et al. 2020

View full text Add to dashboard Cite

C RUDE commercial soybean lecithin from two different sources named (A) and (C) were modified physically, chemically, and enzymatically using phospholipase A 2 enzyme (PLA 2) in order to improve their emulsification properties. All the modified lecithins were used in the preparation of oil-in-water (O/W) emulsions, in comparison with the crude lecithins as well as a standard acetone-insoluble lecithin purchased from the market. The emulsions stabilized by acetone-insoluble portion showed the best physical stability during 3 months of storage after preparation, along with a relatively low particle size (PS) and poly-dispersity index (PDI) and a zeta-potential values that indicates a good emulsion stability. Hydroxylated lecithin (H2A and H2C) showed the most stable Zeta-potential (-55.3 and-50.8 mV) respectively with a relatively low particle size and poly-dispersity index of 284.0 nm and 0.378 respectively for A and 268.4 nm and 0.391 for C, while hydrolyzed lecithin (ENZ A and ENZ C) that possessed the lowest particle size had a moderate zeta-potential of-34.8 and-32.2 for A and C respectively. These results showed that the modification of crude lecithin is essential for the production of tailor-made products with specific surface-active properties-stabilizing emulsions to be used in different purposes.

show abstract

Enzymatic preparation and characterization of soybean lecithin-based emulsifiers

Cited by 3 publications

References 15 publications

Production of Hydrogel-Based Curcumin-Loaded O/W Suspoemulsions

Production of Hydrogel-Based Curcumin-Loaded O/W Suspoemulsions

Suitable simple and fast methods for selective isolation of phospholipids as a tool for their analysis

Comparative study of modified soy lecithins as oil in water (O/W) emulsifiers.

Contact Info

Product

Resources

About