Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from <i>Grifola Frondosa</i>: Natural Killer Cell Cytotoxicity and Antioxidant Activity

Liu, Shiyao; Zhuang, Xiaohong; Zhang, Xiaolin; Han, Wei; Liu, Yuchun; Sun, Donglin; Guo, Wei

doi:10.1111/1750-3841.14148

Cited by 11 publications

(5 citation statements)

References 30 publications

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“…Molecular modification can affect the activity of polysaccharides by changing their spatial structure, molecular weight, substituent type, number and position (Getachew & Chun, 2017;Xie et al, 2020). There are many methods for the modification of polysaccharides, including physical method, chemical method and biological method (Liu et al, 2018;Xie et al, 2020;Zhu et al, 2019). In addition to the modification of the main chain, the branched chain of polysaccharides can also be modified (Lee et al, 2017;Xiao et al, 2020).…”

Section: Results Of Tablementioning

confidence: 99%

Extraction, preparation and an assessment of the activity of carboxymethyl polysaccharide from Panax japonicus

Yu¹,

Yuan²,

Yan³

et al. 2022

Food Sci. Technol

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A response surface method (RSM) was employed to optimize polysaccharide extraction from Panax japonicus (PJPS). Also, carboxymethyl substitution of PJPS (CM-PJPS) was studied using a response surface methodology. A three-variable Box-Behnken design (BBD) methodology was applied. RSM analyses revealed that conditions that maximized polysaccharide yield were as follows: a liquid-to-material ratio 22:1, an extraction temperature of 83.99 °C and an extraction duration of 2.32 h. Under optimum extraction conditions, the average absorbance value of PJPS was 0.6001, and the purity of PJPS was 98.2%. A quadratic regression model for CM-PJPS was obtained via BBD. The optimum extraction conditions for the process were determined to include a 1.38 h reaction time, 1.24 g monochloroacetic acid (MCA) and a reaction temperature of 52.85 °C. Under optimum extraction conditions, the average degree of CM-PJPS substitution was 0.9733. The structure of the PJPS was examined by means of Fourier transform infrared spectroscopy (FTIR). CM-PJPS exhibited the strongest anti-oxidant activity in vitro. In addition, CM-PJPS inhibited Skov3 and A2780 cancer cell proliferation. Our data revealed that CM-PJPS is a promising natural antioxidant with potential value as a food supplement and for treatment of cancer.

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Section: Results Of Tablementioning

confidence: 99%

Extraction, preparation and an assessment of the activity of carboxymethyl polysaccharide from Panax japonicus

Yu¹,

Yuan²,

Yan³

et al. 2022

Food Sci. Technol

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“…MGN-3/Biobran is a bio-modified RBS using the carbohydrate hydrolysing enzymes from shiitake mushrooms [ 23 ]. mRBPSs [ 21 ], polysaccharides with the weight-average molecular weight of 10 4 –10 5 Da, was obtained from the modification of RBS using the intracellular enzymes from Grifola frondosa and could strengthen the natural killer cells’ cytotoxicity to K562 cells. However, the polysaccharides [ 20 ] obtained from the fermentation product of Grifola frondosa - rice bran water extract has the weight-average molecular weight of 10 2 –10 3 Da.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharides from Ganoderma Sinense - rice bran fermentation products and their anti-tumor activities on non-small-cell lung cancer

Han

Chen

Zhou³

et al. 2021

BMC Complement Med Ther

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Background Non-small-cell lung cancer (NSCLC) accounts more than 80% of the lung cancer cases. Polysaccharides in rice bran and its fermentation products have been proven to suppress many cancers. However, the report on inhibiting NSCLC is few. In this paper, the polysaccharides with suppression activity to H1299 NSCLC in the fermentation products of full-fat rice bran and defatted rice bran were studied in vitro and in vivo. Method Polysaccharides (GSRBPs) were extracted from Ganoderma sinense – full-fat rice bran (GS-FRB) and Ganoderma sinense – defatted rice bran (GS-DRB) fermentation products. The structure information of the GSRBPs was studied using HPLC analysis. The anti-tumor activities on H1299 NSCLC of GSRBPs in vitro study was performed using MTT method. The in vivo studies use BALB/c-nu nude mice as H1299 NSCLC bearing mice. Result All the polysaccharides contained two fractions, GSFPS-1 and GSFPS-2. The molecular weight and the ratio of GSFPS-1 and GSFPS-2 were different in GS-FRB and GS-DRB. At the earlier state of fermentation, all polysaccharides were composed of D-glu, D-man, D-xyl and L-ara with certain molar ratios. But at the latter stage, polysaccharides in GS-FRB were composed of D-glu, D-man, D-xyl, L-ara and D-fru, while these in GS-DRB only composed of D-glu and D-man. In the in vitro study, the IC50 of RBS and GSRBPs was as GS-DRB-11 (40.62 μg/mL), GS-FRB-9 (43.82 μg/mL), GS-DRB-7 (48.08 μg/mL), RBS (49.56 μg/mL), GS-DRB-9 (49.91 μg/mL), GS-DRB-13 (51.89 μg/mL), GS-FRB-11 (53.75 μg/mL), GS-FRB-7 (56.84 μg/mL), GS-DRB-13 (60.63 μg/mL) from small to large. In the in vivo study, the H1299 NSCLC inhibition rate (InRa) of RBS and GSRBPs were GS-DRB-11 (86.81%) > GS-DRB-9 (86.01%) > GS-FRB-9 (84.88%) > GS-DRB-7 (82.21%) > GS-DRB-13 (78.04%) > RBS (76.06%) > GS-FRB-13 (65.44%) > GS-FRB-11 (64.70%) > GS-FRB-7 (27.87%). The GSFPS-2 area percent was negatively correlated to the IC50 and was positively correlated to the InRa. This means the GSFPS-2 had much higher anti-tumor activity than GSFPS-1. Conclusion GSFPS-2 had higher anti-tumor activities, and the lipid in the rice bran has a decisive effect on the structures of polysaccharides produced by fermentation. Therefore, GSRBPs could be considered as potential novel agents to suppress H1299 non-small-cell lung cancer.

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“…An excessively high molecular weight can reduce the exposure of the active sites on HPS, thus limiting their bioactivity. However, if the molecular weight is too low, the number of active functional groups becomes insufficient, leading to poor bioactivity [70][71][72]. The monosaccharide composition also plays an important role in the immune activity of HPS.…”

Section: Immunomodulatory Activity Of Hpsmentioning

confidence: 99%

Herb Polysaccharide-Based Drug Delivery System: Fabrication, Properties, and Applications for Immunotherapy

et al. 2022

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Herb polysaccharides (HPS) have been studied extensively for their healthcare applications. Though the toxicity was not fully clarified, HPS were widely accepted for their biodegradability and biocompatibility. In addition, as carbohydrate polymers with a unique chemical composition, molecular weight, and functional group profile, HPS can be conjugated, cross-linked, and functionally modified. Thus, they are great candidates for the fabrication of drug delivery systems (DDS). HPS-based DDS (HPS-DDS) can bypass phagocytosis by the reticuloendothelial system, prevent the degradation of biomolecules, and increase the bioavailability of small molecules, thus exerting therapeutic effects. In this review, we focus on the application of HPS as components of immunoregulatory DDS. We summarize the principles governing the fabrication of HPS-DDS, including nanoparticles, micelles, liposomes, microemulsions, hydrogels, and microneedles. In addition, we discuss the role of HPS in DDS for immunotherapy. This comprehensive review provides valuable insights that could guide the design of effective HPS-DDS.

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Enzymatic Modification of Rice Bran Polysaccharides by Enzymes from Grifola Frondosa: Natural Killer Cell Cytotoxicity and Antioxidant Activity

Cited by 11 publications

References 30 publications

Extraction, preparation and an assessment of the activity of carboxymethyl polysaccharide from Panax japonicus

Extraction, preparation and an assessment of the activity of carboxymethyl polysaccharide from Panax japonicus

Polysaccharides from Ganoderma Sinense - rice bran fermentation products and their anti-tumor activities on non-small-cell lung cancer

Herb Polysaccharide-Based Drug Delivery System: Fabrication, Properties, and Applications for Immunotherapy

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