Enzymatic microassay of serum bile acids: Increased sensitivity with an enzyme amplification technique

Nicolas, Jean‐Claude; Chaintreuil, J; Descomps, Bernard; Paulet, A. Crastes de

doi:10.1016/0003-2697(80)90252-3

Cited by 37 publications

(10 citation statements)

References 9 publications

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“…Precision studies by others found that both assays could determine low concentrations. 2,[9][10][11][12][13][14] Bile acid values from this study are similar to those reported previously. Flammer 6 reported species differences within the same genus; however, species comparisons were not included in this study.…”

Section: Discussionsupporting

confidence: 91%

“…9,10 An enzymatic method has also been developed in which an oxidative reaction of free bile acids results in color change. [11][12][13] Both assays show good specificity and sensitivity for mammals and have been found to have adequate precision and accuracy. 2,[9][10][11][12][13][14] The purpose of this study was to compare the RIA and the enzymatic method for determining bile acid concentrations in plasma samples from several avian species.…”

Section: Introductionmentioning

confidence: 99%

“…[11][12][13] Both assays show good specificity and sensitivity for mammals and have been found to have adequate precision and accuracy. 2,[9][10][11][12][13][14] The purpose of this study was to compare the RIA and the enzymatic method for determining bile acid concentrations in plasma samples from several avian species. We examined the correlation of values between assays, assay linearity, and the effects of hemolysis, lipemia, high lactate dehydrogenase (LDH) concentration, and sample storage.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Two Methods to Determine Plasma Bile Acid Concentrations in Healthy Birds

Cray¹,

Andreopoulos

2003

Journal of Avian Medicine and Surgery

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of Two Methods to Determine Plasma Bile Acid Concentrations in Healthy Birds

Cray¹,

Andreopoulos

2003

Journal of Avian Medicine and Surgery

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“…Norway). Total biliary bile acids were deter mined enzymatically with 3-hvdroxydehydrogenase according to the technique of Nicolas et al [27], The individual biliary' bile acids were analyzed by gas liq uid chromatography [28], Bile samples (100 pi) were hydrolyzed overnight in 4.0 ml of 2.5 N NaOH in sealed tubes at 100 °C. The tubes were cooled on ice.…”

Section: A Nalytical Proceduresmentioning

confidence: 99%

Development of Bile Secretory Function in the Neonatal Guinea Pig

Tuchweber

Ducruet²,

Perea³

et al. 1990

Neonatology

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Maturation of hepatic bile formation and lipid secretion were examined in neonatal guinea pigs in an attempt to explain the earlier observation that the neonate (15 days old) is less susceptible to lithocholate-induced cholestasis than the adult. Bile flow and bile acid secretion were lowest at 12 h postpartum and increased to attain a maximum at 15 days. Thereafter, values decreased to a level which was not significantly different from that at 3 days of age. Bile acid analysis indicated the presence of chenodeoxycholic acid, 7-ketolithocholic acid and ursodeoxycholic acid in bile at all ages. But, at 15 days ursodeoxycholic acid was a major contributor to total bile acid secretion. Phospholipid and cholesterol secretion increased between the 1st and the 3rd day of age but decreased markedly at 15 days. This indicated an uncoupling of biliary lipid secretion and endogenous bile acids which was related in part to the nature of the bile acids secreted. Serum bile acid levels were markedly increased at 15 days of age. This hypercholanemia may reflect a rapid expansion of the bile acid pool including an enhanced bile acid secretion which could explain in part the resistance to the lithocholate cholestasis by increasing the secretory capacity for the toxic bile acid.

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“…The final concen tration of the bile acids was calculated on the basis of reference to the internal standard [11]. Plasma bile acids were determined by the enzyme method [13], Student's t test was used for the statistical analysis, with p > 0.05 indicating no significant difference.…”

Section: Introductionmentioning

confidence: 99%

Bile Acid Composition in Neonatal Life in Rats

Yousef¹,

Tuchweber²

1982

Neonatology

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Bile acid (BA) metabolism was studied in the intestine, liver and plasma of rats at 0–1 day (neonates); 14 days (preweaning); 22 days (weaning) and 60 days (young adults) of age, as well as in the maternal tissues. In the intestine, total BA content (μmol/l00g body weight) decreased during neonatal life and reached maternal levels at 60 days. In the liver, BA increased up to 14 days and then decreased at 22 and 60 days of age, but these concentrations were higher than those found in the neonate and the mother. The serum concentrations in 14-and 22-day-old rats were more than 30 times higher than in the newborns. At 60 days, serum BA content was still twice that of the mother. BA composition in the intestine and the liver showed a reduction in 12α-hydroxylated BA associated with an increase in chenodeoxycholic acid and 6β-hydroxylated BA during neonatal life. These data indicate an increased synthesis of chenodeoxycholic and 6β-hydroxylated BA after birth. It further suggests a possible defect in excretory mechanisms in neonatal life.

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Enzymatic microassay of serum bile acids: Increased sensitivity with an enzyme amplification technique

Cited by 37 publications

References 9 publications

Comparison of Two Methods to Determine Plasma Bile Acid Concentrations in Healthy Birds

Comparison of Two Methods to Determine Plasma Bile Acid Concentrations in Healthy Birds

Development of Bile Secretory Function in the Neonatal Guinea Pig

Bile Acid Composition in Neonatal Life in Rats

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