Comparison of Two Methods to Determine Plasma Bile Acid Concentrations in Healthy Birds

Cray, Carolyn; Andreopoulos, Angela

doi:10.1647/2002-022

Cited by 7 publications

(4 citation statements)

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“…High overall prevalences of Aspergillus and Chlamydia psittaci exposure in this population were not surprising given the ubiquitous nature of Aspergillus spp. spores in the environment, the worldwide distribution of C. psittaci in migratory birds, and prolonged antibody persistence for these pathogens (Aguilar and Redig, 1995;Cray and Tatum, 1998;Kaleta and Taday, 2003;Hubalek, 2004). Although aspergillosis has been recognized as a cause of mortality in water birds (Astorga et al, 1994;Lagerquist et al, 1994) and is frequently recognized as a disease of recently captured or captive birds (Souza and Degernes, 2005), the Aspergillus-positive oystercatchers captured in this study did not exhibit signs of clinical disease.…”

Section: Discussionmentioning

confidence: 74%

“…The following blood values were measured: glucose, sodium, potassium, carbon dioxide, creatinine, total protein, phosphorus, calcium, uric acid, creatine phosphokinase (CPK), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), lipase, amylase, gammaglutamyl transferase (GGT), cholesterol, and triglycerides. Protein fractions were evaluated by electrophoresis and plasma bile acid was measured by radioimmunoassay (RIA; University of Miami, Miami, Florida, USA; Cray and Tatum, 1998;Cray and Andreopoulos, 2003). Chlamydophila psittaci antibody titers were determined by indirect immunofluorescence and Aspergillus IgG-antibody titers and antigen titers were determined by enzyme-linked immunosorbent assay (ELISA; University of Miami, Miami, Florida, USA; Cray et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

“…Protein fractions were evaluated by electrophoresis and plasma bile acid was measured by radioimmunoassay (RIA; University of Miami, Miami, Florida, USA; Cray and Tatum, 1998;Cray and Andreopoulos, 2003). Chlamydophila psittaci antibody titers were determined by indirect immunofluorescence and Aspergillus IgG-antibody titers and antigen titers were determined by enzyme-linked immunosorbent assay (ELISA; University of Miami, Miami, Florida, USA; Cray et al, 2009). Plasma samples were tested for West Nile Virus (WNV) antibodies (Allison et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

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Health Assessment of American Oystercatchers (Haematopus Palliatus Palliatus) in Georgia and South Carolina

Carlson-Bremer

Norton²,

Gilardi

et al. 2010

Journal of Wildlife Diseases

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ABSTRACT:The American Oystercatcher (Haematopus palliatus palliatus) is the only species of oystercatcher native to the Atlantic coast of North America and is restricted in distribution to intertidal shellfish beds in coastal areas. Currently, the American Oystercatcher population in South Carolina and Georgia is threatened by widespread habitat loss, resulting in low reproductive success and small population size. Oystercatchers could be an important indicator of ecosystem health because they depend on quality coastal breeding habitat and prey on bivalves, which can accumulate toxins and pathogens from the local environment. Data were collected from American Oystercatchers (n5171) captured at five sites in South Carolina and Georgia between 2001 and 2006. Iridial depigmentation was frequently noted during physical examination and was more prevalent in female birds. Female birds were larger than males on average, but ranges for weight and morphometric measurements had considerable overlap. Mean values were calculated for hematology, plasma biochemistry, and hormone levels, and prevalence of exposure to select pathogens was determined. Mercury was the only trace metal detected in blood samples. These data provide baseline health information needed for longitudinal monitoring and conservation efforts for American Oystercatchers. In addition, this study illustrates the potential use of this species as an indicator for the health of the southeastern US coastal nearshore ecosystem.

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Section: Discussionmentioning

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Health Assessment of American Oystercatchers (Haematopus Palliatus Palliatus) in Georgia and South Carolina

Carlson-Bremer

Norton²,

Gilardi

et al. 2010

Journal of Wildlife Diseases

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“…At present, the function of the liver in captive reptiles is monitored on the basis of biopsy and microscopic examination of the tissue (Schildger 1999, Divers and Cooper 2000). However, the hepatic function of mammals and birds has frequently been monitored by measuring the serum concentrations of bile acids (Center 1993, Lumeij 1997, Harr 2002, Cray and Andreopoulos 2003), and a serum bile acid assay has been adapted for terrestrial tortoises (Montesinos and others 2002).…”

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confidence: 99%

Fasting and postprandial serum bile acid concentrations in 10 healthy female red‐eared terrapins (Trachemys scripta elegans)

Knotková

Dorrestein²,

Jekl

et al. 2008

Veterinary Record

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The fasting and postprandial serum concentrations of bile acids and other blood constituents were measured in a group of 10 clinically healthy, female, six-year-old captive red-eared terrapins (Trachemys scripta elegans). The terrapins were housed in a temperate room and maintained in four aquaria in which the water temperature ranged from 24 to 27 degrees C and the temperature above the basking site ranged from 27 to 30 degrees C. The serum concentrations of bile acids were measured four times in a period of five months, and at the second sampling the fasting and two postprandial (after 24 and 48 hours) serum concentrations of total protein, albumin, glucose, uric acid, cholesterol, triglycerides, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and bile acids were determined. Coelioscopy revealed vitellogenic and previtellogenic follicles on the ovaries of all the terrapins, and eggs with calcified shells were detected in two of them. The livers were mostly pink to deep yellow in colour, with sharp edges, a smooth serosal surface, distinct large superficial vessels, and multifocal melanin deposits. Liver biopsies revealed fine, more or less oil red O-positive lipid droplets in all the hepatocytes, but in none of the cases was it considered to be pathological lipidosis. The mean (sd) bile acid concentrations ranged from 7.35 (4.52) to 10.04 (7.40) micromol/l. The fasting and postprandial concentrations were 3.1 (2.3), 4.5 (5.4) (24 hours) and 2.2 (1.5) (48 hours) micromol/l. High concentrations between 27.6 and 66.6 micromol/l were associated with lipaemia. There were no significant differences between the biochemical profiles of the fasting and postprandial serum samples.

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Avian Digestive System Disorders

Oglesbee¹

2006

Saunders Manual of Small Animal Practice

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Comparison of Two Methods to Determine Plasma Bile Acid Concentrations in Healthy Birds

Cited by 7 publications

References 14 publications

Health Assessment of American Oystercatchers (Haematopus Palliatus Palliatus) in Georgia and South Carolina

Health Assessment of American Oystercatchers (Haematopus Palliatus Palliatus) in Georgia and South Carolina

Fasting and postprandial serum bile acid concentrations in 10 healthy female red‐eared terrapins (Trachemys scripta elegans)

Avian Digestive System Disorders

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