Enzymatic kinetics for lipase‐catalyzed hydrolysis of water‐insoluble substrate in AOT reverse micelles, involving native and activated lipases

Talukder, Md. Mahabubur Rahman; Zaman, Masihuz; Hayashi, Yoshinori; Wu, Jicang; Kawanishi, Takuya; Ogino, Chiaki; Shimizu, Nobuaki

doi:10.1002/jctb.972

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…141 It has been shown that the addition of non-ionic surfactants, such as Spans, Tweens, short-chain PEG and alkyl glycosides can increase the activity of the Chromobacterium viscosum lipase in an Aerosol OT/isooctane-based microemulsion. 142,143 Interestingly, both the catalytic activity and stability of the same lipase were increased by the presence of aprotic solvents in the Aerosol OT/isooctane-based microemulsions, dimethyl sulfoxide being the best solvent. 144 The half-life was increased from 33 days without solvent to 125 days with dimethyl sulfoxide.…”

Section: Surfactant-based Lipase Preparationsmentioning

confidence: 99%

Immobilisation and application of lipases in organic media

2013

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Different methods of preparing lipases for use in organic media are critically reviewed. Solid lipase preparations can be made by typical immobilisation methods such as adsorption, entrapment, covalent coupling or cross-linking. Immobilisation is especially attractive for lipases because, in addition to the normal benefits of enzyme immobilisation, it can also lead to a considerable increase in catalytic activity, probably caused by conformational changes in the lipase molecules. Activation can be achieved, for example, using hydrophobic support materials or surfactants during the immobilisation procedure. Surfactants can also be used to solubilise lipases in organic media via the formation of hydrophobic ion pairs, surfactant-coated lipase or reversed micelles. Lipase preparation methods are discussed with regard to potential lipase inactivation and activation effects, mass transfer limitations, lipase stability and other features important for applications. The practical applications of lipases in organic media reviewed include ester synthesis, modification of triacylglycerols and phospholipids, fatty acid enrichment, enantiomer resolution, biodiesel production and acylation of carbohydrates and bioactive compounds.

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Section: Surfactant-based Lipase Preparationsmentioning

confidence: 99%

Immobilisation and application of lipases in organic media

2013

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“…AOT (dioctyl sodium sulfosuccinate) and PEG ( polyethylene glycol) 400 were used to stabilize the enzyme and create the correct environment within the matrix. 20 The charged head group on AOT has the potential to interact with the enzyme, and the PEG forms stable microemulsions within a gel matrix. 21 An acidic sol-gel method was also used to entrap lipase B employing PEG 400 to stabilise the enzyme and phosphoric acid to catalyse the gelation.…”

Section: Changing the Enzyme And Bio-chemo Compatibility Entrapping T...mentioning

confidence: 99%

Minimizing side reactions in chemoenzymatic dynamic kinetic resolution: organometallic and material strategies

et al. 2012

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Chemoenzymatic dynamic kinetic resolution (DKR) of rac-1-phenyl ethanol into R-1-phenylethanol acetate was investigated with emphasis on the minimization of side reactions. The organometallic hydrogen transfer (racemization) catalyst was varied, and this was observed to alter the rate and extent of oxidation of the alcohol to form ketone side products. The performance of highly active catalyst [(pentamethylcyclopentadienyl)IrCl(2)(1-benzyl,3-methyl-imidazol-2-ylidene)] was found to depend on the batch of lipase B used. The interaction between the bio- and chemo-catalysts was reduced by employing physical entrapment of the enzyme in silica using a sol-gel process. The nature of the gelation method was found to be important, with an alkaline method preferred, as an acidic method was found to initiate a further side reaction, the acid catalyzed dehydration of the secondary alcohol. The acidic gel was found to be a heterogeneous solid acid.

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“…Ample research has also been devoted to the field of reverse micellar systems for enzymatic triglyceride hydrolysis (Kim and Chung, 1989;Talukder et al, 2004). However, due to the difficulty of adequately characterizing the interface, few kinetic models have been developed and applied to the hydrolysis of triglycerides in O/W emulsion, despite the practical importance of this system (Martinez et al, 1992;Tsai et al, 1991).…”

Section: Introductionmentioning

confidence: 99%