Enzymatic determination of serum-free fatty acids: A colorimetric method

Shimizu, Sakayu; Tani, Yoshiki; Yamada, Hideaki; Tabata, Masayoshi; Murachi, T

doi:10.1016/0003-2697(80)90511-4

Cited by 231 publications

(72 citation statements)

References 13 publications

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“…Serum non-esteri®ed fatty acid (NEFA) concentration was measured enzymatically. 20 Measurement of¯ow-mediated and nitroglycerininduced dilatation of the brachial artery Flow-mediated dilatation (FMD) and nitroglycerin (NTG)-induced dilatation of the brachial artery were measured by an examiner who was unaware of the subjects' clinical settings, except for their physical appearance. Studies were undertaken in a quiet and temperature-controlled (22±23 C) room, according to the method described before.…”

Section: Methodsmentioning

confidence: 99%

The impairment of flow-mediated vasodilatation in obese men with visceral fat accumulation

et al. 1998

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BACKGROUND: Obesity has been reported to be associated with coronary artery disease and other atherosclerotic diseases. Recently, evidence has accumulated indicating that intra-abdominal visceral fat accumulation contributes to atherogenesis; however, the mechanism underlying this remains to be determined. This study was undertaken to elucidate whether intra-abdominal visceral fat accumulation impairs vascular endothelial function in obese men. METHODS AND RESULTS: Thirty-eight obese men (body mass index (BMI) ! 26.0), aged 19±64 y (mean age 37.6 AE 1.8 y) and 23 age-matched non-obese subjects were examined. According to the ratio of the maximum thickness of preperitoneal fat to the minimum thickness of subcutaneous fat (Pmax/Smin) obtained by longitudinal ultrasound scanning in the subxiphoid region in obese men, we divided obese subjects into two categories; visceral (PmaxaSmin ! 1; n 23) and subcutaneous type (PmaxaSmin`1; n 15). To investigate endothelial function, we performed ultrasound measurement of the brachial artery diameter non-invasively both at rest and during reactive hyperaemia in the muscle distal to the brachial artery which causes endothelium-dependent vasodilatation. The brachial diameter change was also measured after sublingual administration of nitroglycerin, which causes endothelium-independent vasodilatation. Flow-mediated diameter (D) increase (%FMD; DDaD Â 100), in the subjects with visceral type obesity (3.09 AE 0.43%) was signi®cantly lower than those of the subjects with subcutaneous type obesity and non-obese subjects (7.90 AE 0.51%, 8.91 AE 0.44%, respectively, P`0.01). The magnitude of endothelium-independent vasodilatation by nitroglycerin was similar in all groups. On multiple regression analysis, the PmaxaSmin showed a signi®cant inverse correlation with %FMD. CONCLUSIONS: The subjects with visceral type obesity, rather than those with the subcutaneous type, are associated with impaired¯ow-mediated endothelium-dependent vasodilatation of the brachial artery.

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Section: Methodsmentioning

confidence: 99%

The impairment of flow-mediated vasodilatation in obese men with visceral fat accumulation

et al. 1998

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“…We used a Hitachi 704 automatic chemical analyzer (Boehringer Mannheim GmbH, Mannheim, Germany) to perform serum biochemical analyses with the procedures and reagents supplied by the company: albumin (Doumas et al, 1971), total protein (procedure proposed by Boehringer), free fatty acids (Shimizy et al, 1980), cholesterol (Siedel et al, 1983;Kattermann et al, 1984), triglycerides (Siedel et al, 1993), ␤-hydroxybutyrate (Bergmeyer and Bernt, 1965), and uric acid (procedure proposed by Boehringer). We did not conduct duplicate analyses because serum samples were too small (1-2 ml).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Estimation of Carcass Fat and Protein in Northern Pintails (Anas Acuta) During Spring Migration

Dombrowski

Bourgeois

Couture

et al. 2003

Journal of Wildlife Diseases

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“…After extraction, the carboxylic groups can be determined by titrimetry (Dole, 1956) or by manual (Laurell and Tibbling, 1967 ;Duncombe, 1963 ;Mosinger, 1965 ;Novak, 1965) or automatic (Antonis, 1965 ;Baird, Black and Faulkner, 1967) colorimetry. Recently, several enzymatic techniques have been developed that use acyl-Co-A synthetase for plasma NEFA specific assay without previous extraction (Mizuno et a/., 1980 ;Okabe et al, 1980 ;Shimizu et al, 1980 ;Hosaka et al, 19811. The reason why there are so many methods for determining NEFA is that there are a number of still unresolved difficulties in obtaining both a quantitative and a specific assay (without interfering substances). The method must be sensitive, rapid, reproducible and applicable to all animal species.…”

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confidence: 99%

“…Methodological studies on herbivore plasma are scarce (Crane and Lane, 1977 ;De Villiers, Van Der Walt and Procos, 1977 ;Bas, 1984) ; most have been carried out on human or rat plasma. Very few methods have been compared to a specific and quantitative reference method (Duncombe and Rising, 1973 ; Donald-Gibson and Young, 1974 ;Brunck and Swanson, 19811, the majority (ltaya and Ui, 1965 ;Laurell and Tibbling, 1967 ;Mosinger, 1965 ;Howorth, Gibbard and Mark, 1966 ;Lorch and Gey, 1966 ;Baird, Black and Faulkner, 1967 ;Dalton and Kowalski, 1967 ;Kashket, 1971 ;Soloni and Sardina, 1973 ;Elphick, 1975 ;Bowyer, Cridland and King, 1978 ;Shimizu et al, 1980) being contrasted with the titrimetric determination of Dole and Meinertz (1960) or Trout, Estes and Friedberg (1960). Enzymatic techniques were mainly compared with the colorimetric determination of copper soaps (Mizuno et al, 1980 ;Shimizu et al, 1980 ;Hosaka et al, 1981 ;Bas, 1984) HIA method (heptane-isopropanol-sulfuric acid).…”

mentioning

confidence: 99%

Determination of plasma non-esterified fatty acids in herbivores and man : a comparison of values obtained by manual or automatic chromatographic, titrimetric, colorimetric and enzymatic methods

Chilliard¹,

Bauchart²,

Barnouin³

et al. 1984

Reprod. Nutr. Dévelop.

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Summary. Non-esterified fatty acid (NEFA) contents were determined (1) in cow, goat, mare and human plasmas or sera and (2) in bovine serum albumin solutions by thin-layer and gas-liquid chromatographic methods which were compared with 6 chemical methods of determination and 2 enzymatic methods (manual and automatic). The chemical methods combined two extraction techniques (isopropanol-heptane and silicic acid-diisopropyl ether) and three determination methods (titrimetry and colorimetry using either phenol red or copper soaps). Correlations between the nine various methods were high, but in some cases regression lines were different from the bissector and species-dependent. The putative occurrence of interfering compounds or the possible effect of between-species differences in NEFA composition have been discussed. The choice of a fast method depends primarily on practical considerations, but the need to standardize each method for a given species has been stressed. Automatic enzymatic assay seemed to be suitable for routine analyses.Introduction.

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Enzymatic determination of serum-free fatty acids: A colorimetric method

Cited by 231 publications

References 13 publications

The impairment of flow-mediated vasodilatation in obese men with visceral fat accumulation

The impairment of flow-mediated vasodilatation in obese men with visceral fat accumulation

Estimation of Carcass Fat and Protein in Northern Pintails (Anas Acuta) During Spring Migration

Determination of plasma non-esterified fatty acids in herbivores and man : a comparison of values obtained by manual or automatic chromatographic, titrimetric, colorimetric and enzymatic methods

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