Enzymatic Determination of Ethanol Using ‘<i>Reagentless</i>’ Electrocatalyzed Luminol Chemiluminescence

Atwater, James E.; Akse, James R.; DeHart, Jeffrey; Wheeler, Richard R.

doi:10.1080/00032719708001666

Cited by 16 publications

(2 citation statements)

References 18 publications

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“…Some analytical methods have been developed during the years for the determination of ethanol and glucose. Enzymatic methods (Gonchar et al, 2001;Atwater et al, 1997), colorimetry (Zanon et al, 2007), as well as advanced techniques such as high performance liquid chromatography (Tagliaro et al, 1991;Lefebvre et al, 2002) and gas chromatography (Buckee and Mundy, 1993;McLachlan et al, 1999) are widely used for determination of ethanol.…”

Section: Introductionmentioning

confidence: 99%

Preparation and optimization of a bienzymic biosensor based on self-assembled monolayer modified gold electrode for alcohol and glucose detection

Asav

Akyılmaz

2010

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Preparation and optimization of a bienzymic biosensor based on self-assembled monolayer modified gold electrode for alcohol and glucose detection

Asav

Akyılmaz

2010

Biosensors and Bioelectronics

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“…Basically, in clinical and industrial laboratories, ethanol and glucose are determined by classical methods, such as colorimetry, spectrophotometry, , chemiluminescence, , and enzymatic methods, , which are poorly selective and time consuming. Chromatographic methods − are very selective and reliable, but they require extensive sample pretreatment and expensive and cumbersome equipment.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous and Accurate Real-Time Monitoring of Glucose and Ethanol in Alcoholic Drinks, Must, and Biomass by a Dual-Amperometric Biosensor

Mentana

Palermo

Nardiello

et al. 2012

J. Agric. Food Chem.

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In this work the optimization and application of a dual-amperometric biosensor for simultaneous monitoring of glucose and ethanol content, as quality markers in drinks and alcoholic fermentation media, are described. The biosensor is based on glucose oxidase (GOD) and alcohol oxidase (AOD) immobilized by co-cross-linking with bovine serum albumin (BSA) and glutaraldehyde (GLU) both onto a dual gold electrode, modified with a permselective overoxidized polypyrrole film (PPYox). Response, rejection of interferents, and stability of the dual biosensor were optimized in terms of PPYox thickness, BSA, and enzyme loading. The biosensor was integrated in a flow injection system coupled with an at-line microdialysis fiber as a sampling tool. Flow rates inside and outside the fiber were optimized in terms of linear responses (0.01-1 and 0.01-1.5 M) and sensitivities (27.6 ± 0.4 and 31.0 ± 0.6 μA·M(-1)·cm(-2)) for glucose and ethanol. Excellent anti-interference characteristics, the total absence of "cross-talk", and good response stability under operational conditions allowed application of the dual biosensor in accurate real-time monitoring (at least 15 samples/h) of alcoholic drinks, white grape must, and woody biomass.

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Dissolved oxygen determination by electrocatalysed chemiluminescence with in-line solid phase media

Atwater

DeHart

Wheeler

1998

J. Biolumin. Chemilumin.

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Dissolved elemental oxygen is determined in a flowing aqueous stream using glucose oxidase to catalyse the reaction between D-glucose and O2 to produce hydrogen peroxide. The levels of the resulting H2O2 are detected and quantified by luminol chemiluminescence using in-line solid phase media for pH adjustment of the reagent stream and for controlled release of the luminophore. The reaction is initiated by electrochemical catalysis. By the use of excess D-glucose in the reagent flow stream, the intensity of chemiluminescence is rendered proportional only to fluctuations in the dissolved O2 concentration. The methodology provides a means for the detection of aqueous O2 in the range 0-10 mg/L.

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Enzymatic Determination of Ethanol Using ‘Reagentless’ Electrocatalyzed Luminol Chemiluminescence

Cited by 16 publications

References 18 publications

Preparation and optimization of a bienzymic biosensor based on self-assembled monolayer modified gold electrode for alcohol and glucose detection

Preparation and optimization of a bienzymic biosensor based on self-assembled monolayer modified gold electrode for alcohol and glucose detection

Simultaneous and Accurate Real-Time Monitoring of Glucose and Ethanol in Alcoholic Drinks, Must, and Biomass by a Dual-Amperometric Biosensor

Dissolved oxygen determination by electrocatalysed chemiluminescence with in-line solid phase media

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