Enzymatic determination of biotin

Haarasilta, Sampsa

doi:10.1016/0003-2697(78)90680-2

Cited by 12 publications

(2 citation statements)

References 17 publications

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“…Biotin has been determined using a radiometricmicrobiological assay, liquid chromatography with uorimetric detection, chemiluminescence energy transfer, polarographic methods (Bonjour 1991), a high-performance liquid chromatography/avidin-binding assay (Zempleni et al 1996), uorometic methods (Lin and Kirsch 1979), an isotope dilution assay using 3 H-Biotin (Dakshinamurti and Allan 1979), an isotope dilution assay using 14 C-Biotin (Hood 1979), enzymatic methods (Haarasilta 1978), and colorimetric procedures (Plinton et al 1969). Thin-layer chromatography has been used to determine Biotin in the presence of water-soluble vitamins (Gröningsson and Jansson 1979).…”

Section: Methodsmentioning

confidence: 99%

Final Report on the Safety Assessment of Biotin

Fiume¹

2001

Int J Toxicol

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Biotin is a water-soluble vitamin used as a hair-conditioning agent and a skin-conditioning agent in many cosmetic products at concentrations ranging from 0.0001% to 0.6%. Although Biotin does absorb some ultraviolet (UV) radiation, the absorption shows no peaks in the UVA or UVB region. Biotin is rapidly metabolized and excreted in urine. Little acute oral toxicity is seen in animal tests. Short-term and subchronic toxicity studies likewise found no evidence of toxicity. Although intradermal injection of a small quantity of Biotin (0.1 ml) into guinea pig skin did not produce skin irritation, Biotin (0.1% at pH 7.3) did produce slight, transient ocular irritation in rabbit eyes. Biotin was not mutagenic in bacterial tests, but positive results were found in a Tradescantia micronucleus test. There was evidence of an increase in the number of resorptions in rats receiving Biotin by subcutaneous injection, with concomitant decreases in fetal, uterine, and placental weights. Another study of mice receiving Biotin orally or by subcutaneous injection found no differences between control and treatment groups. Although there is one case study reporting an urticarial reaction in the literature, there are a very large number of individuals exposed to Biotin on a daily basis, and there is not a parallel appearance of irritation, sensitization, or other adverse reactions. Based on these available data, it was concluded that Biotin is safe as used in cosmetic formulations.

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Section: Methodsmentioning

confidence: 99%

Final Report on the Safety Assessment of Biotin

Fiume¹

2001

Int J Toxicol

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“…The main role for this enzyme seems to be an element of the regulation of the tricarboxylic acid cycle and the incorporation of aspartate in proteins. When biotin is absent from the medium, the pyruvate carboxylase activity is much reduced [5,26], the enzyme subsisting as a non-functional apoprotein [26][27][28].…”

Section: Increase Of L-mafic Acid Degradation and Reduction Of Pyruvamentioning

confidence: 99%

Anabolic role of ?-malic acid in Saccharomyces cerevisiae in anaerobiosis during alcoholic fermentation

Salmon¹

1987

FEMS Microbiology Letters

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Biotin-Active Compounds, Their Existence in Nature and the Biotin Requirements of Yeasts

Oura

Suomalainen

1982

Journal of the Institute of Brewing

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After a short account of the discovery of biotin and the progress of early biotin research, the natural occurrence of biotin with particular consideration to the raw materials used in the fermentation industry and its products is described. Of the many known biotin derivatives, those appearing in nature and those which can be converted to biotin‐active (or inactive) compounds by simple procedures are reported. Ways to by‐pass the need for biotin in microbes is discussed. The importance of biotin in yeast production, the biotin requirements of yeast, and the effect of culture conditions on these requirements are reported. The close relationship between the participation of biotinylenzymes and the biotin requirements is noted.

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Enzymatic determination of biotin

Cited by 12 publications

References 17 publications

Final Report on the Safety Assessment of Biotin

Final Report on the Safety Assessment of Biotin

Anabolic role of ?-malic acid in Saccharomyces cerevisiae in anaerobiosis during alcoholic fermentation

Biotin-Active Compounds, Their Existence in Nature and the Biotin Requirements of Yeasts

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