Enzymatic degradation of bacterial biofilms using Aspergillus clavatus MTCC 1323

Singh, Vineeta; Verma, Nishi; Banerjee, Banani; Kumari, Vibha; Haque, Shafiul; Tripathi, C. K. M.

doi:10.1134/s0026261715010130

Cited by 22 publications

(14 citation statements)

References 15 publications

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“…In a recent study, using the latter Singh et al . () demonstrated that Aspergillus clavatus produced a mixture of amylases, proteases and pectinases that reduced biofilms of Ps. aeruginosa and B. subtilis by 82% and 75%, respectively.…”

Section: Biological Methodsmentioning

confidence: 99%

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

Phillips

2016

Int J of Food Sci Tech

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Biofilms are immobile communities of micro-organisms attached to any surface, such as stainless steel or a food matrix surface or on packaging material. They may be composed of a single species, but more generally, in the natural environment, they consist of mixed species together with an extracellular matrix. Biofilms provide a common mechanism of persistence for a number of bacterial species especially in food processing environments, and therefore, prevention of biofilm formation and the removal of preformed biofilms are an important issue for the food industry. This article reviews the current understanding on the formation of biofilms and recent developments in biological and chemical methods for prevention and removal.

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Section: Biological Methodsmentioning

confidence: 99%

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

Phillips

2016

Int J of Food Sci Tech

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“…The EPS consists of various biopolymers, out of which heterogeneous polysaccharides are one of the predominant components. Strategies making use of enzymes including individual (Loiselle andAnderson 2003, Nijland et al 2010;Kalpana et al 2012;Lamppa and Griswold 2013) as well as mixture of enzymes (Orgaz et al 2006;Orgaz et al 2007;Singh et al 2015;Fleming, et al 2017) have been investigated to combat biofilms. Lequette et al (2010) analyzed the cleaning efficiency of different polysaccharidases and proteolytic enzymes against bacterial biofilms commonly found in the food industry processing lines.…”

Section: Introductionmentioning

confidence: 99%

“…Lequette et al (2010) analyzed the cleaning efficiency of different polysaccharidases and proteolytic enzymes against bacterial biofilms commonly found in the food industry processing lines. Efficacy of proteases and carbohydrases like cellulases, amylase and pectinase have also been evaluated against the biofilms of Pseudomonas sp., Bacillus subtilis and Staphylococcus aureus (Orgaz et al 2007;Singh et al 2015) which these organisms may form in the clinical settings as well as on medical devices. Therefore, keeping in view (i) the heterogeneity of the extracellular polysaccharides on the biofouled surfaces and, (ii) the cost incurred in procuring the purified enzyme preparations, a mixture of enzymes may be more beneficial for sufficient disruption of bacterial biofilms and clearing of slime.…”

Section: Introductionmentioning

confidence: 99%

A novel multi-enzyme preparation produced from Aspergillus niger using biodegradable waste: a possible option to combat heterogeneous biofilms

et al. 2020

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Extracellular polymeric substance (EPS) produced by the microorganisms provides protection and stability to them when they are encased within biofilms. Heterogeneous polysaccharides form a major constituent of the EPS and are crucial for the formation and integrity of the biofilms/slime. Thus, breakdown of polysaccharides might help in dispersion of biofilms from abiotic surfaces. In the present study we isolated a fungus, Aspergillus niger APS, capable of concurrently producing a cocktail of carbohydrases and optimized the conditions for higher yields of all the enzymes by one variable at a time (OVAT) approach. The optimization studies resulted in 1.5 to 12 fold augmentation in the enzyme yields using biodegradable waste. Further, keeping in view the heterogeneous nature of polysaccharides in biofilm matrix, the in-house produced enzyme cocktail was used for the dispersal of biofilms formed by Salmonella enterica serovar Typhi, Escherichia coli and Staphylococcus aureus. Treatment with enzyme preparation caused 90.23 ± 4.0, 82.64 ± 5.0 and 76.32 ± 5.0% reduction of the biofilms formed by these organisms respectively which was also evidenced by Field emission scanning electron microscopy (FESEM) revealing the loss of biofilm architecture. Interestingly, the enzyme cocktail could also remove viscous slime formed under natural conditions in the kitchen drainage pipe (KDP). To the best of our knowledge, this is the first report on biotreatment of abiotic surfaces for removal of biofilms/slime formed under natural conditions. The study thus indicates the prospects of using multiple carbohydrases as an anti-biofouling agent on abiotic surfaces like equipments as well as implants/prostheses and pipelines.

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“…It is very difficult to treat the wounds infected with S. aureus due to its multidrug resistance and natural tendency of biofilm formation. Watters et al [10] tested the efficacy of the enzymes like α-amylase, bromelain, lysostaphin against methicillin resistant strains of S. aureus biofilms and found that all of them decreased the biomass significantly within 24 h. The fungal strain, Aspergillus clavatus MTCC 1323 was found to produce enzyme complex (protease, amylase, pectinase) which degrades the biofilms of P. aeruginosa, B. subtilis and S. aureus [11]. It is reported that glycoside hydrolases proved to be potential therapeutic agents exhibiting activity against diverse microorganisms by degrading biofilms [12].…”

Section: Introductionmentioning

confidence: 99%

“…Aspergillus clavatus MTCC 1323 produce enzyme preparation with protease, amylase and pectinase activities under solid state fermentation. This enzyme degraded the biofilms of Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus after 7 days of incubation[11]. Newly formulated enzyme cleaners, known as Deconex Prozyme Active consisted of mixture of four enzymes viz.…”

mentioning

confidence: 99%

Bacterial Biofilm Degradation Using Extracellular Enzymes Produced by <i>Penicillium janthinellum</i> EU2D-21 under Submerged Fermentation

Nagraj¹,

Gokhale²

2018

AiM

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Bacterial biofilms are the bacterial aggregates that are embedded in the self-produced matrix of extracellular polymeric substances (EPS) that cause persistent bacterial infections posing significant medical challenges. They are recalcitrant to antibiotics and host defenses which make the treatments difficult and costly. Penicillium janthinellum mutant EU2D-21 was found to produce extracellular enzyme complex (amylase, cellulase, protease) under submerged fermentation. Maximum specific enzyme activities were found to be 3.04 IU/mg, 2.61 IU/mg and 3.39 IU/mg for alpha-amylase, cellulase and protease respectively, after 8 days of incubation at 30˚C. We evaluated the enzyme complex for its ability to target and degrade the biofilms of different bacteria. We found that it degraded biofilms of Escherichia coli (85.5%), Salmonella enterica (79.72%), Pseudomonas aeruginosa (88.76%) and Staphyloccus aureus (87.42%) within 1 h of incubation at 50˚C. The scanning electron microscopy (SEM), quantitation of biofilm removal assay and Crystal violet assay demonstrated that the enzyme complex detached the biofilm exo-polysaccharide matrix and bacteria from the cell surface. These results illustrate the feasibility and benefits of using this enzyme complex as anti-biofilm therapeutics to eradicate biofilms. This can also be used as a promising strategy to improve treatment of multidrug resistant bacterial infections.

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Enzymatic degradation of bacterial biofilms using Aspergillus clavatus MTCC 1323

Cited by 22 publications

References 15 publications

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

A novel multi-enzyme preparation produced from Aspergillus niger using biodegradable waste: a possible option to combat heterogeneous biofilms

Bacterial Biofilm Degradation Using Extracellular Enzymes Produced by <i>Penicillium janthinellum</i> EU2D-21 under Submerged Fermentation

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Enzymatic degradation of bacterial biofilms using Aspergillus clavatus MTCC 1323

Cited by 22 publications

References 15 publications

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

A novel multi-enzyme preparation produced from Aspergillus niger using biodegradable waste: a possible option to combat heterogeneous biofilms

Bacterial Biofilm Degradation Using Extracellular Enzymes Produced by &lt;i&gt;Penicillium janthinellum&lt;/i&gt; EU2D-21 under Submerged Fermentation

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Bacterial Biofilm Degradation Using Extracellular Enzymes Produced by <i>Penicillium janthinellum</i> EU2D-21 under Submerged Fermentation