Enzymatic Cross-Linking of Dynamic Thiol-Norbornene Click Hydrogels

Nguyen, Han; Liu, Hung‐Yi; Hudson, Britney N.; Lin, Chien‐Chi

doi:10.1021/acsbiomaterials.8b01607

Cited by 27 publications

(36 citation statements)

References 65 publications

(149 reference statements)

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“…The CLE procedure has their catalytic activity reduced if the enzymes immobilized, because they are solubilize in the solution, discussed previously. To achieve the crystallized enzyme in the CLEC procedure, to be immobilized which must be purified highly, the procedure formed very costly and complex [ 184 , 185 ]. So, the CLEAs appeared as a substitute to the procedures defined in the literature already.…”

Section: Immobilization Of Lipasesmentioning

confidence: 99%

“…With a crosslinking agent blending spray-dried enzymes the cross-linked spray-dried enzymes (CSDEs) are produced. In this method, a polymer (carrier particles) a solution/suspension containing the enzyme is fed into a spray dryer; to escape deterioration of enzymes due to disclosure to high temperatures these particles are used during drying [ 184 ]. To a medium containing the crosslinking agent the enzymes are added so that crosslinking occurs after drying.…”

Section: Immobilization Of Lipasesmentioning

confidence: 99%

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Microbial lipases and their industrial applications: a comprehensive review

et al. 2020

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Lipases are very versatile enzymes, and produced the attention of the several industrial processes. Lipase can be achieved from several sources, animal, vegetable, and microbiological. The uses of microbial lipase market is estimated to be USD 425.0 Million in 2018 and it is projected to reach USD 590.2 Million by 2023, growing at a CAGR of 6.8% from 2018. Microbial lipases (EC 3.1.1.3) catalyze the hydrolysis of long chain triglycerides. The microbial origins of lipase enzymes are logically dynamic and proficient also have an extensive range of industrial uses with the manufacturing of altered molecules. The unique lipase (triacylglycerol acyl hydrolase) enzymes catalyzed the hydrolysis, esterification and alcoholysis reactions. Immobilization has made the use of microbial lipases accomplish its best performance and hence suitable for several reactions and need to enhance aroma to the immobilization processes. Immobilized enzymes depend on the immobilization technique and the carrier type. The choice of the carrier concerns usually the biocompatibility, chemical and thermal stability, and insolubility under reaction conditions, capability of easy rejuvenation and reusability, as well as cost proficiency. Bacillus spp., Achromobacter spp., Alcaligenes spp., Arthrobacter spp., Pseudomonos spp., of bacteria and Penicillium spp., Fusarium spp., Aspergillus spp., of fungi are screened large scale for lipase production. Lipases as multipurpose biological catalyst has given a favorable vision in meeting the needs for several industries such as biodiesel, foods and drinks, leather, textile, detergents, pharmaceuticals and medicals. This review represents a discussion on microbial sources of lipases, immobilization methods increased productivity at market profitability and reduce logistical liability on the environment and user.

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Section: Immobilization Of Lipasesmentioning

confidence: 99%

Section: Immobilization Of Lipasesmentioning

confidence: 99%

Microbial lipases and their industrial applications: a comprehensive review

et al. 2020

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“…共价交联水凝胶可由多种化学和酶交联剂合成 [19] , 如β-二酮类、丙烯酸酯类、丙烯酰胺类化学交 [20] . 光催化凝胶的快速形成为改变交联密度以及选择性地结合生物分子提供了一种新的方法, 但光引发剂的毒性、光照射使细胞活性降低、不同透光率使凝胶结构和厚度差异显著等缺陷限制了其进一步应用 [21,22] . 酶促凝胶的聚合不仅可以在温和的反应条件下通过调节大分子组成来控制凝胶化, 还可以通过调节酶动力学来控制凝胶化 [23] .…”

Section: 凝胶化及酶促聚合反应unclassified

“…Figure 3 Frmation of TU-Cat gel and related interactions [33] (color online). 图 4 PEG水凝胶的合成及降解原理图 [21] (网络版彩图) 图 5 HRP/H 2 O 2 诱导巯基生成及巯基-去冰片烯交联的原理图 [22] (网络版彩图) 图 6 HRP引发体系中中间体形成的循环机制 [34] (网络版彩图)…”

Section: 过氧化物酶和漆酶等因在酶促循环体系中能产生unclassified

Synthesis of enzymatic hydrogels and their application in biosensors

Miao¹,

Pan²,

Zhu³

et al. 2021

Sci. Sin.-Chim

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In recent years, polymer-based hydrogels have become the unique electrode modification material, which provides a new idea for the design of new biosensors. Furthermore, enzymes have been used to catalyze the polymerization of various hydrogels due to their high specificity, low toxicity and high catalytic efficiency. In this paper, we discussed several synthesis methods of enzymatic hydrogels and their potential application in cell metabolites, tissue engineering, wound healing, and cancer surveillance. Concluding remarks and future prospects regarding preparation and application of biosensor based on enzymatic gel reaction were provided at the end.

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“…[276] More recently, Nguyen et al used HRP/H2O2 to cross-link 8-arm PEG-norbornene with bis-cysteinebearing peptides in presence of murine NIH/3T3 fibroblasts. [277] Tyrosinase is another oxidative enzyme able to cross-link phenol and catechol-functionalized molecules in the absence of hydrogen peroxide (Fig. 25).…”

Section: Enzyme-mediated Ligationmentioning

confidence: 99%

Chemical cross-linking methods for cell encapsulation in hydrogels

Echalier

Valot

Martínez

et al. 2019

Materials Today Communications

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Cell-encapsulating hydrogels are of tremendous interest in regenerative medicine. Tissue engineering relies on biomaterials able to act as artificial extracellular matrices to guide cells towards the development of new tissues. Therefore, considerable efforts have been made to design biomaterials which mimic cells' native environment, thus encouraging natural behavior. The choice of biomaterial in which cells are embedded is crucial for their survival, proliferation and differentiation. Being more stable, chemical hydrogels are preferred over physical hydrogels as cell-laden substrates. When designing chemical hydrogels, scientists must choose not only the nature of the network (synthetic and/or biopolymers) but also the type of cross-link bridging hydrogel constituents. For that purpose, numerous chemistries have been used (i) to introduce reactive functions on the hydrogel precursors and (ii) to form covalent bonds in the presence of living cells. The review will discuss the advantages and limitations of each strategy.

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Enzymatic Cross-Linking of Dynamic Thiol-Norbornene Click Hydrogels

Abstract: , C.-C. (2019). Enzymatic crosslinking of dynamic thiol-norbornene click hydrogels. ACS Biomaterials Science & Engineering.

Cited by 27 publications

References 65 publications

Microbial lipases and their industrial applications: a comprehensive review

Microbial lipases and their industrial applications: a comprehensive review

Synthesis of enzymatic hydrogels and their application in biosensors

Chemical cross-linking methods for cell encapsulation in hydrogels

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