Enzymatic characterization of Nocardia spp. and related bacteria by API ZYM profile

Boiron, P.; Provost, F.

doi:10.1007/bf00442770

Cited by 26 publications

(10 citation statements)

References 22 publications

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“…In 1990, Boiron and Provost further evaluated the API ZYM system for the detection of constitutive enzymes on chromogenic substrates with 62 isolates belonging to the genus Nocardia and related bacteria (65). Results from this study were consistent with those of Kilian's study (288), except for the reactions with N. brasiliensis.…”

Section: Recent Advances Rapid Identificationsupporting

confidence: 69%

The medically important aerobic actinomycetes: epidemiology and microbiology

1994

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The aerobic actinomycetes are soil-inhabiting microorganisms that occur worldwide. In 1888, Nocard first recognized the pathogenic potential of this group of microorganisms. Since then, several aerobic actinomycetes have been a major source of interest for the commercial drug industry and have proved to be extremely useful microorganisms for producing novel antimicrobial agents. They have also been well known as potential veterinary pathogens affecting many different animal species. The medically important aerobic actinomycetes may cause significant morbidity and mortality, in particular in highly susceptible severely immunocompromised patients, including transplant recipients and patients infected with human immunodeficiency virus. However, the diagnosis of these infections may be difficult, and effective antimicrobial therapy may be complicated by antimicrobial resistance. The taxonomy of these microorganisms has been problematic. In recent revisions of their classification, new pathogenic species have been recognized. The development of additional and more reliable diagnostic tests and of a standardized method for antimicrobial susceptibility testing and the application of molecular techniques for the diagnosis and subtyping of these microorganisms are needed to better diagnose and treat infected patients and to identify effective control measures for these unusual pathogens. We review the epidemiology and microbiology of the major medically important aerobic actinomycetes.

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Section: Recent Advances Rapid Identificationsupporting

confidence: 69%

The medically important aerobic actinomycetes: epidemiology and microbiology

1994

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“…T but not ATCC 19247 T (19,64,117,132,163,190,191,192,193,194,196). Because of these findings, the International Judicial Committee continued to use the name N. farcinica, although the species distinction between N. farcinica and N. asteroides remained controversial.…”

Section: History Of Nocardiamentioning

confidence: 99%

Clinical and Laboratory Features of theNocardiaspp. Based on Current Molecular Taxonomy

et al. 2006

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SUMMARY The recent explosion of newly described species of Nocardia results from the impact in the last decade of newer molecular technology, including PCR restriction enzyme analysis and 16S rRNA sequencing. These molecular techniques have revolutionized the identification of the nocardiae by providing rapid and accurate identification of recognized nocardiae and, at the same time, revealing new species and a number of yet-to-be-described species. There are currently more than 30 species of nocardiae of human clinical significance, with the majority of isolates being N. nova complex, N. abscessus, N. transvalensis complex, N. farcinica, N. asteroides type VI (N. cyriacigeorgica), and N. brasiliensis. These species cause a wide variety of diseases and have variable drug susceptibilities. Accurate identification often requires referral to a reference laboratory with molecular capabilities, as many newer species are genetically distinct from established species yet have few or no distinguishing phenotypic characteristics. Correct identification is important in deciding the clinical relevance of a species and in the clinical management and treatment of patients with nocardial disease. This review characterizes the currently known pathogenic species of Nocardia, including clinical disease, drug susceptibility, and methods of identification.

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“…Then, approximately 5 mL of distilled water was distributed into each cupule of the honeycombed wells of the tray. After two drops of the suspension were dispensed into each cupule (65 µL), the strips were incubated for 4 h at 37 o C. The API ZYM test was conducted twice in order to ensure reproducibility of the results (Boiron and Provost, 1990).…”

Section: Api Zym Testsmentioning

confidence: 99%

Characterization of Bacillus mojavensis KJS-3 for industrial applications

et al. 2011

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One of the strains of the Bacillus mojavensis group, Bacillus mojavensis KJS-3 (B. mojavensis KJS-3), which has been demonstrated to play a role in protecting plants against diseases as a bacterial endophyte and in reducing the accumulation of mycotoxins generated by an endophytic fungus, was recently discovered in food waste. In this study, the identification and characterization of B. mojavensis KJS-3 was performed via TEM analysis, API-zym test, API 50 CHB test, assays of catalase and oxidase activity, lactic acid production, stability under various conditions, antibiotic susceptibility, and cellular fatty acid composition. The overall results of this study demonstrate that B. mojavensis KJS-3 may have great potential as a probiotic product, as this bacterium is quite stable in somewhat harsh environments. B. mojavensis KJS-3 was positive on oxidase and catalase tests, and the conversion rate of glucose to lactic acid was 58.9%. Finally, anteiso-C(15:0) (43.10%) was identified as the major fatty acid.

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Enzymatic characterization of Nocardia spp. and related bacteria by API ZYM profile

Abstract: Characterization of 62 isolates belonging to the genus Nocardia and related bacteria was obtained by using the API ZYM system. The difference in enzymatic profile should allow a relatively efficient, low-cost means to identify aerobic actinomycetes of clinical significance.

Cited by 26 publications

References 22 publications

The medically important aerobic actinomycetes: epidemiology and microbiology

The medically important aerobic actinomycetes: epidemiology and microbiology

Clinical and Laboratory Features of theNocardiaspp. Based on Current Molecular Taxonomy

Characterization of Bacillus mojavensis KJS-3 for industrial applications

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