Characterization of Bacillus mojavensis KJS-3 for industrial applications

Choi, Soyeon; Park, Min Hi; Jung, Taehun; Moon, Kyung Ho; Kim, Kang Min; Kang, Jae Seon

doi:10.1007/s12272-011-0215-z

Cited by 10 publications

(8 citation statements)

References 43 publications

(41 reference statements)

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“…Phenotypic characterizations were conducted as described by Bergey [26], and carbon source utilization was tested with API 50 CH carbohydrate fermentation strips according to the manufacturer’s instructions (bioMérieux, Inc.) [27]. A whole cell fatty acid analysis was performed as described by Sharma [28].…”

Section: Methodsmentioning

confidence: 99%

Antifungal Activity of Isolated Bacillus amyloliquefaciens SYBC H47 for the Biocontrol of Peach Gummosis

Yanzhou

Wei

et al. 2016

PLoS ONE

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The gummosis disease is caused by Botryosphaeria dothidea (Moug. ex. Fr) Ces. et de Not., and it is one of the most important diseases of stone fruits worldwide. The use of biocontrol as an alternative approach to synthetic chemical fungicides has aroused general concern about how to control plant diseases that are caused by phytopathogens. The aim of this study is to isolate Bacillus strains from raw honeys with the capacity to inhibit B. dothidea and to explore the mechanisms by which they could be used in the biocontrol of peach gummosis. Bacillus amyloliquefaciens SYBC H47 was isolated and identified on the basis of its physiological and biochemical characteristics and its 16S rRNA and gyrB gene sequences. The cell suspension and the cell-free supernatant of its culture showed significant antifungal activity against Aspergillus niger, Mucor racemosus, Fusarium oxysporum, Penicillium citrinum, and Candida albicans by agar-diffusion assays. The primary antifungal substances were bacillomycin L, fengycin, and surfactin, which were analyzed by HPLC LC/ESI-MS/MS. Bacillomycin L showed the best inhibitory effect against conidial germination of B. dothidea, followed by fengycin and surfactin. Surfactin had limited effects on mycelial growth, contrary to those of bacillomycin L and fengycin. However, a mixture of the three lipopeptides had a synergistic effect that disrupted the structure of the conidia and mycelia. In order to reduce the production cost, the use of waste frying peanut oil and soy oil as the sole carbon source increased the lipopeptide yield levels by approximately 17% (2.42 g/L) and 110% (4.35 g/L), respectively. In a field trial, the decreases in the infected gummosis rate (IGR) and the disease severity index (DSI) through cell suspension treatments were 20% and 57.5% (in 2014), respectively, and 40% and 57.5% (in 2015), respectively, in comparison with the control. In conclusion, B. amyloliquefaciens SYBC H47 could inhibit the germination of conidia and the growth of mycelia from B. dothidea; therefore, this strain behaves as a potential biocontrol agent against the gummosis disease.

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Section: Methodsmentioning

confidence: 99%

Antifungal Activity of Isolated Bacillus amyloliquefaciens SYBC H47 for the Biocontrol of Peach Gummosis

Yanzhou

Wei

et al. 2016

PLoS ONE

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“…found in food waste and has been registered in the Korean Culture Center of Microorganisms (KCCM) under the accession number KCCM10961P [5] with specific patterns of isoquinones. Previous studies have shown that B. mojavensis KJS-3 exhibits almost all typical characteristics of B. mojavensis strains: the KJS-3 strain bacteria are rod-shaped, Gram-positive, and endospore-forming aerobic bacteria.…”

Section: B Mojavensis Kjs-3 a New B Mojavensis Strain Was Initiallymentioning

confidence: 99%

“…Bacillus species are used in many medical, pharmaceutical, agricultural, and industrial processes that take advantage of the bacteria's wide range of physiological characteristics and their ability to produce a host of enzymes, antibiotics, and other metabolites [5,12,13,16]. The rhizosphere, a narrow region of soil that is directly influenced by root secretions, is associated with microbial activity [3].…”

Section: B Mojavensis Kjs-3 a New B Mojavensis Strain Was Initiallymentioning

confidence: 99%

“…mojavensis KJS-3 endospores were produced using the method described by Choi et al [5]. Endospore powder (0.01 g) was added to 1 ml sterile distilled water to produce a bacterial suspension.…”

Section: Ethanol Tolerancementioning

confidence: 99%

“…With increasing human development, soil salinization is one of the most important problems resulting from land degradation and basic environmental problems in arid and semi-arid regions [10]. The NaCl concentration [5]. Ethanol is commonly used to kill bacteria including B. mojavensis KJS-3.…”

Section: Stability Of B Mojavensis Kjs-3mentioning

confidence: 99%

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Characterization of Bacillus mojavensis KJS-3 for the Promotion of Plant Growth

Kim¹,

Liu²,

Go³

et al. 2015

Journal of Life Science

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Scientists have recently shown an interest in the characteristics of Bacillus mojavensis strains because of their increasing use in plants as a defense against diseases and mycotoxins. We have shown here that B. mojavensis KJS-3 possesses the typical characteristics of B. mojavensis strains including a strong resistance to high temperatures (≤50°C), tolerance to high salt concentrations (7% NaCl), ethanol tolerance (40% ethanol), and pH range for growth (pH 5-9). B. mojavensis KJS-3 has been used for the production of cyclic lipopeptides including important antifungal substances such as surfactin, iturin, and fengycin. Polymerase chain reaction analysis in this study showed that B. mojavensis KJS-3 can be used for the production of fengycin and the findings of LC-MS/MS analyses suggest that B. mojavensis KJS-3 can be used to produce iturin and surfactin. Antifungal activity analys is confirmed that B. mojavensis KJS-3 has antifungal effects on Botrytis cinerea, Rhizoctonia solani AG-4, Sclerotinia sclerotiorum, and Colletotricum goeosporioides. A microscopy assessment of the roots of wild ginseng plants planted together with B. mojavensis KJS-3 revealed that the roots contained B. mojavensis KJS-3, confirming the bacteria to be a plant growth promoting endophyte (PGPE) which acts against plant diseases and mycotoxins. Our findings lead us to conclude that B. mojavensis KJS-3 can be produced at an industrial level as a microbial pesticide or microbial fertilizer.

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