Enzymatic Characterization ofCorynespora cassiicolaIsolates Using theAPI‐ZYM^®System

Abstract

Corynespora cassiicola (Berk. & Curt.) Wei is an important phytopathogenic fungus, and different isolates show great diversity in their reproductive structures. Therefore, the aim of this study was to evaluate the potential of the API‐ZYM® system as an auxiliary tool in the polyphasic approach of C. cassiicola identification. Five C. cassiicola isolates from different host plants and one Pseudocercospora griseola isolate were tested. A typical enzymatic pattern was obtained, with eight enzymes being produced by all five C. cassiicola isolates. An intraspecific differentiation was also found. Certain enzymes produced, such as α‐glucosidase, β‐glucosidase and α‐mannosidase, may be related to pathogenic processes, acting in the degradation of the structural components of the host cells.

Effects of Conventional and High‐Oleic Peanut Medium on Extracellular Enzyme Production and Mycelial Growth of Aspergillus Flavus

“…Five discs (5 mm in diameter) containing the mycelia of each isolate were then transferred to the flask containing CP and HOP medium and incubated for 15 days at 25C under a 12‐h light/12‐h dark period (Howe and Jander ; Oliveira et al . ). The cultures were filtered through Whatman filter paper to obtain a filtrate free of fungal structures and sterilized using Millipore filters (pore size of 0.22 µm).…”

Distribuição de fungos e ácido tenuazônico em grãos de sorgo cultivados em diferentes épocas de semeadura e estudo polifásico de cepas de <i>Phoma</i> spp. isoladas.

“…Para avaliar a atividade enzimática através deste sistema, 5 discos de micélio (5 mm de diâmetro), oriundos das bordas das colônias de 65 isolados de E. sorghinum previamente cultivados em meio sorgo por 7 dias, foram depositados em erlenmeyers contendo 100 mL de meio líquido batata dextrose (Difco) e incubados à 25 °C (OLIVEIRA et al, 2013). Após o período de incubação, o meio líquido fermentado por cada isolado foi filtrado através de membrana de 0,22 µm (Millipore) e utilizados para análises dos perfis de produção enzimática.…”