Enzymatic biosensors for the detection of water pollutants

Reynoso, Eduardo C.; Romero-Guido, Cynthia; Rebollar-Pérez, Georgette; Torres, Eduardo

doi:10.1016/b978-0-12-824436-4.00012-5

Cited by 6 publications

(4 citation statements)

References 98 publications

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“…AChE is found in all organisms including insects, animals, reptiles, and fish, which degrades the acetylcholine (ACh), a neurotransmitting agent, and is catalyzed into inactive chemicals such as choline and acetic acid [ 68 , 69 , 70 ]. Recent studies predicted that activity of AChE was also suppressed in P. xylostella samples after treatment with different concentrations of Me@FL-SiO 2 NPs-70 nm and -150 nm, while activity remained high in the case of methoxyfenozide (TC)-treated samples, as shown in Figure 5 D.…”

Section: Resultsmentioning

confidence: 99%

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

Bilal

Sial

Cao

et al. 2022

IJMS

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The diamond back moth, Plutella xylostella, causes severe damage at all crop stages, beside its rising resistance to all insecticides. The objective of this study was to look for a new control strategy such as application of insecticide-loaded carbon dot-embedded fluorescent mesoporous silica nanoparticles (FL-SiO2 NPs). Two different-sized methoxyfenozide-loaded nanoparticles (Me@FL-SiO2 NPs-70 nm, Me@FL-SiO2 NPs-150 nm) were prepared, with loading content 15% and 16%. Methoxyfenozide was released constantly from Me@FL-SiO2 NPs only at specific optimum pH 7.5. The release of methoxyfenozide from Me@FL-SiO2 NPs was not observed other than this optimum pH, and therefore, we checked and controlled a single release condition to look out for the different particle sizes of insecticide-loaded NPs. This pH-responsive release pattern can find potential application in sustainable plant protection. Moreover, the lethal concentration of the LC50 value was 24 mg/L for methoxyfenozide (TC), 14 mg/L for Me@FL-SiO2 NPs-70 nm, and 15 mg/L for Me@FL-SiO2 NPs-150 nm after 72 h exposure, respectively. After calculating the LC50, the results predicted that Me@FL-SiO2 NPs-70 nm and Me@FL-SiO2 NPs-150 nm exhibited better insecticidal activity against P. xylostella than methoxyfenozide under the same concentrations of active ingredient applied. Moreover, the activities of detoxification enzymes of P. xylostella were suppressed by treatment with insecticide-loaded NPs, which showed that NPs could also be involved in reduction of enzymes. Furthermore, the entering of FL-SiO2 NPs into the midgut of P. xylostella was confirmed by confocal laser scanning microscope (CLSM). For comparison, P. xylostella under treatment with water as control was also observed under CLSM. The control exhibited no fluorescent signal, while the larvae treated with FL-SiO2 NPs showed strong fluorescence under a laser excitation wavelength of 448 nm. The reduced enzyme activities as well as higher cuticular penetration in insects indicate that the nano-based delivery system of insecticide could be potentially applied in insecticide resistance management.

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Section: Resultsmentioning

confidence: 99%

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

Bilal

Sial

Cao

et al. 2022

IJMS

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“…The principle of operation is based on the relationship between the toxicity of a pesticide and the decrease in enzyme activity. Quantitative measurement of enzymatic activity is performed before and after exposure to a particular analyte [67]. After exposure to a certain pesticide, this activity is measured by an appropriate transduction technique, e.g., amperometry, spectrometry, fluorometry, potentiometry, etc., depending on the products of the activity.…”

Section: (B) Enzyme Biosensorsmentioning

confidence: 99%

Integrating Wireless Remote Sensing and Sensors for Monitoring Pesticide Pollution in Surface and Groundwater

Mutunga,

Sinanovic,

Harrison

2024

Sensors

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Water constitutes an indispensable resource crucial for the sustenance of humanity, as it plays an integral role in various sectors such as agriculture, industrial processes, and domestic consumption. Even though water covers 71% of the global land surface, governments have been grappling with the challenge of ensuring the provision of safe water for domestic use. A contributing factor to this situation is the persistent contamination of available water sources rendering them unfit for human consumption. A common contaminant, pesticides are not frequently tested for despite their serious effects on biodiversity. Pesticide determination in water quality assessment is a challenging task because the procedures involved in the extraction and detection are complex. This reduces their popularity in many monitoring campaigns despite their harmful effects. If the existing methods of pesticide analysis are adapted by leveraging new technologies, then information concerning their presence in water ecosystems can be exposed. Furthermore, beyond the advantages conferred by the integration of wireless sensor networks (WSNs), the Internet of Things (IoT), Machine Learning (ML), and big data analytics, a notable outcome is the attainment of a heightened degree of granularity in the information of water ecosystems. This paper discusses methods of pesticide detection in water, emphasizing the possible use of electrochemical sensors, biosensors, and paper-based sensors in wireless sensing. It also explores the application of WSNs in water, the IoT, computing models, ML, and big data analytics, and their potential for integration as technologies useful for pesticide monitoring in water.

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“…The biosensors employ a biological recognition element (bioreceptor), can be a whole cell-base system or cell partbased system, for the detection of toxic pollutants, these devices allow onside quantification and avoid the requirement of sophisticated equipment [31]. The development of a wide type of biosensors using different bioreceptors such as microbial fuel cells [18,[32][33][34][35][36], microorganisms (Whole cell-based) [37][38][39][40], antibodies [41][42][43], enzymes [44][45][46][47][48][49][50], among others has been reported in the literature. On the other hand, biosensors can be placed as a favorable alternative due to the variability of pollutants (Heavy metals, organic compounds, drugs, and microorganisms) that are capable of pollutant trace detection [29].…”

Section: Introductionmentioning

confidence: 99%

Immobilized Enzyme-based Novel Biosensing System for Recognition of Toxic Elements in the Aqueous Environment

et al. 2023

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Access to secure water sources has become one of the biggest challenges for human sustainability. Climate change and associated droughts make it difficult to guarantee the usual water source and move to groundwater use or to the re-use of treated wastewater remains unviable due the lack on the capacity of monitoring water quality. Moreover, reusing treated wastewater from repositories near anthropogenic sources represents a risk of high concentrations of emerging contaminants. The strategies involve a higher risk of encountering toxic elements with a heavy burden on human and environmental health. New accessible and reliable tools are required to detect any hazard from the waterbodies in real time to ensure safe management and also to decrease mismanagement or ilegal water discharges. One of the available options is to look into enzyme-based biosensors that can detect toxic elements in the water. The proposed biosensors require sensible elements to be accessible and durable for their proper function. The present revision shows in first place, the actual need of real time monitoring due the different sources and effects of emergent pollutants. Secondly, describes how enzymes can be immobilized for its application in biosensors and the rol enzymes play as bioreceptor element in biosensing. Thirdly, describes the transduction methods that can be observed, and finally the actual application of enzyme biosensors for the detection of different toxic elements. According to the presented literature enzyme-based biosensors have been successfully applied for the detection of a wide number of pollutants reaching detection limits comparable to traditional methods such as up to 0.018 nM of mercury. Furthermore, laccase seems to be the more applied enzyme in literature, but positive results are not limited to this enzyme and other candidates have been explored showing good detection rate.

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Enzymatic biosensors for the detection of water pollutants

Cited by 6 publications

References 98 publications

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

Integrating Wireless Remote Sensing and Sensors for Monitoring Pesticide Pollution in Surface and Groundwater

Immobilized Enzyme-based Novel Biosensing System for Recognition of Toxic Elements in the Aqueous Environment

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