Enzymatic activity studies of Pseudomonas cepacia lipase adsorbed onto copolymer supports containing β-cyclodextrin

Dhake, Kishor P.; Karoyo, Abdalla H.; Mohamed, Mohamed H.; Wilson, Lee D.; Bhanage, Bhalchandra M.

doi:10.1016/j.molcatb.2012.10.011

Cited by 35 publications

(32 citation statements)

References 41 publications

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“…Immobilization by adsorption onto a polymer matrix was also studied by Dhake, Karoyo, Mohamed, Wilson, and Bhanage (). In this case, the support was a copolymer based on polyurethane and β‐cyclodextrin (β‐CD).…”

Section: Immobilization and Improvement Of The Catalytic Propertiesmentioning

confidence: 99%

Burkholderia cepacia lipase: A versatile catalyst in synthesis reactions

Sánchez

Tonetto

Ferreira

2017

Biotech & Bioengineering

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The lipase from Burkholderia cepacia, formerly known as Pseudomonas cepacia lipase, is a commercial enzyme in both soluble and immobilized forms widely recognized for its thermal resistance and tolerance to a large number of solvents and short-chain alcohols. The main applications of this lipase are in transesterification reactions and in the synthesis of drugs (because of the properties mentioned above). This review intends to show the features of this enzyme and some of the most relevant aspects of its use in different synthesis reactions. Also, different immobilization techniques together with the effect of various compounds on lipase activity are presented. This lipase shows important advantages over other lipases, especially in reaction media including solvents or reactions involving short-chain alcohols.

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Section: Immobilization and Improvement Of The Catalytic Propertiesmentioning

confidence: 99%

Burkholderia cepacia lipase: A versatile catalyst in synthesis reactions

Sánchez

Tonetto

Ferreira

2017

Biotech & Bioengineering

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“…Enzymatic membrane bioreactors (EMBRs), endowed with synergistic catalysis and separation functions, can create a favorable thermodynamic and kinetic process. In recent years, EMBRs have grasped much attention in fields of food and medical industry, chemical manufacturing, and environmental protection [1][2][3][4][5][6]. Generally, EMBRs have the characteristics of elongating the lifetime of enzymes and improving their reusability, and the porous structure of membranes allows for a high specific surface area and relatively low diffusion resistance.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane

Chen

Zhu

et al. 2019

Catalysts

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Enzymatic membrane bioreactors (EMBRs) possess the characteristic of combining catalysis with separation, and therefore have promising application potentials. In order to achieve a high-performance EMBR, membrane property, as well as operating parameters, should give special cause for concerns. In this work, an EMBR based on hollow fiber polysulfone microfiltration membranes with radial gradient pore structure was fabricated and enzyme immobilization was achieved through pressure-driven filtration. Lipase from Candida rugosa was used for immobilization and EMBR performance was studied with the enzymatic hydrolysis of glycerol triacetate as a model reaction. The influences of membrane pore diameter, substrate feed direction as well as operational parameters of operation pressure, substrate concentration, and temperature on the EMBR activity were investigated with the production of hydrolysates kinetically fitted. The complete EMBR system showed the highest activity of 1.07 × 104 U⋅g−1. The results in this work indicate future efforts for improvement in EMBR.

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“…Thus, cross-linked CDs offer a potential alternative as dual adsorbent/immobilization materials for biotechnology and environmental remediation ( cf . Graphical Abstract ) due to their tunable pore structure, surface area (SA), and surface chemical properties (Dhake et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Cyclodextrin-Based Polymer-Supported Bacterium for the Adsorption and in-situ Biodegradation of Phenolic Compounds

2018

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Dual function polymer materials with immobilized Sphingobium Chorophenolicum (SpC) bacterium cells are reported herein that undergo tandem adsorption and biodegradation of phenolic compounds. The cross-linked polymer materials contain β-cyclodextrin (β-CD) with incremental hexamethylene diisocyanate (HDI) cross-linker at variable mole ratios (X = 1, 3, or 6), denoted as HDI-X systems. The adsorptive uptake properties of the insoluble HDI-X polymers (X = 3 and 6) with various phenolic compounds [pentachlorophenol (PCP), 2,4,6-trichlorophenol (TCP), and 2,4,6-trimethylphenol (TMP)] were studied using batch adsorption isotherms. The molecular selective phenol removal (SR) capacity of the HDI-3 and HDI-6 materials was evaluated by electrospray ionization mass spectrometry (ESI-MS). The results were compared against granular activated carbon (GAC) and native β-CD, where 1D/2D 1H NMR spectral characterization of the complexes formed between phenolic guests and a soluble polymer (HDI-1) in aqueous solution provide insight on the intermolecular interactions and the role of cross-linking effects. Immobilization of SpC onto HDI-3 was shown to form a composite polymer/bacterium material. The composite system displays synergistic removal effects due to tandem PCP adsorption and SpC biodegradation to yield by-products such as 2,6-dichloro-1,4-hydroquinone (DCHQ). Apoptosis and cytotoxicity of DCHQ were evaluated using three breast cancer cell lines.

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Enzymatic activity studies of Pseudomonas cepacia lipase adsorbed onto copolymer supports containing β-cyclodextrin

Cited by 35 publications

References 41 publications

Burkholderia cepacia lipase: A versatile catalyst in synthesis reactions

Burkholderia cepacia lipase: A versatile catalyst in synthesis reactions

Fabrication and Optimization of a Lipase Immobilized Enzymatic Membrane Bioreactor based on Polysulfone Gradient-Pore Hollow Fiber Membrane

Cyclodextrin-Based Polymer-Supported Bacterium for the Adsorption and in-situ Biodegradation of Phenolic Compounds

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