Environmentally-friendly strategy for separation of α-lactalbumin from whey by aqueous two phase flotation

Jiang, Bin; Wang, Lele; Na, Jiaxin; Zhang, Xiaoqing; Yuan, Yongqiang; Liu, Chunhong; Feng, Zhibiao

doi:10.1016/j.arabjc.2018.11.013

Cited by 30 publications

(22 citation statements)

References 39 publications

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“…Rather than using alcohol-based LBS, a recent study had showed the extraction of α-Lactalbumin from whey used a different phase forming component (i.e., PEG 1000 and citrate salts) along with bubble-assisted technologies showed a separation efficiency and purification fold of 87.54% and 5.33 [64]. The advantages of this study had showed the feasibility of bubble-assisted technology compared to conventional liquid-liquid extraction providing a low processing cost, rapid, and good separation yield.…”

Section: Bubble-assisted Lbsmentioning

confidence: 99%

Liquid Biphasic System: A Recent Bioseparation Technology

et al. 2020

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A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers’ interest for being an alternative bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed. The key parameters affecting the LBS were presented and evaluated. Moreover, future prospect and challenges were highlighted to be a useful guide for future development of LBS. The efforts presented in this review will provide an insight for future researches in liquid-liquid separation techniques.

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Section: Bubble-assisted Lbsmentioning

confidence: 99%

Liquid Biphasic System: A Recent Bioseparation Technology

et al. 2020

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“…It can enhance the viability of probiotic cells during processing, storage, subsequent consumption, and gastrointestinal digestion [ 6 , 7 ]. It has been reported that different methods such as emulsification, phase separation, and spray drying have been used to microencapsulate bioactive substances [ 7 , 8 , 9 , 10 ]. However, many of these methods involve high temperatures or a large amount of organic reagents, which may adversely affect the survival rate of probiotics or cause potential hazards to the human body.…”

Section: Introductionmentioning

confidence: 99%

Targeting Delivery System for Lactobacillus Plantarum Based on Functionalized Electrospun Nanofibers

Liu

et al. 2020

Polymers

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With the increased interest in information on gut microbes, people are realizing the benefits of probiotics to health, and new technologies to improve the viability of probiotics are still explored. However, most probiotics have poor resistance to adverse environments. In order to improve the viability of lactic acid bacteria, polylactic acid (PLA) nanofibers were prepared by coaxial electrospinning. The electrospinning voltage was 16 kV, and the distance between spinneret and collector was 15 cm. The feed rates of the shell and core solutions were 1.0 and 0.25 mL/h, respectively. The lactic acid bacteria were encapsulated in the coaxial electrospun nanofibers with PLA and fructooligosaccharides (FOS) as the shell materials. Scanning electron microscopy, transmission electron microscopy, and laser scanning confocal microscopy showed that lactic acid bacteria were encapsulated in the coaxial electrospun nanofibers successfully. The water contact angle test indicated that coaxial electrospun nanofiber films had good hydrophobicity. An in vitro simulated digestion test exhibited that the survival rate of lactic acid bacteria encapsulated in coaxial electrospun nanofiber films was more than 72%. This study proved that the viability of probiotics can be improved through encapsulation within coaxial electrospun PLA nanofibers and provided a novel approach for encapsulating bioactive substances.

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“…The Pv was analyzed by SDS-PAGE (Bio-Rad Co., Ltd., Guangzhou, China) as described by Jiang [36,37]. The resolving gel concentration was 12%, and the concentration of the stacking gel was 5%, and the molecular weight of the pre-stained protein Marker ranged from 14.4~116.0 kDa.…”

Section: Methodsmentioning

confidence: 99%

Ultrasonic Thermal-Assisted Extraction of Phosvitin from Egg Yolk and Evaluation of Its Properties

Jiang

Wang

et al. 2019

Polymers

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Phosvitin (Pv) is the principal phosphoprotein in chicken egg yolk and the most highly phosphorylated protein in nature. Pv is a good natural food antioxidant and emulsifier. However, the current extraction methods present disadvantages of complicated operation and are time-consuming. In this paper, Pv was extracted from the egg yolk by ultrasonic thermal-assisted extraction (UTAE). The effects of heating time, ultrasonic power and ultrasonic time on the extraction of Pv were investigated by a single factor. The purity of Pv, ratio of nitrogen to phosphorus (N/P), and activity were used as evaluation indexes. An efficient extraction of Pv was achieved when the sample was heated for 15 min at 80 °C and then processed for 10 min of ultrasonic treatment with an ultrasonic power of 600 W. Under optimal conditions, the purity and activity of Pv were 80% and 98%, respectively, whereas the ratio of N/P was 3.1. The obtained Pv was identified by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), Fluorescence analyses, fourier-transform infrared (FT-IR), and liquid chromatography-nanoelectrospray ionization mass spectrometry (Nano LC-ESI-MS/MS) analysis. The results showed there is no significant difference in the properties of Pv obtained by UTAE and Pv standard. The developed extraction approach is a simple, industrial compatible method without the use of any organic solvents.

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Environmentally-friendly strategy for separation of α-lactalbumin from whey by aqueous two phase flotation

Cited by 30 publications

References 39 publications

Liquid Biphasic System: A Recent Bioseparation Technology

Liquid Biphasic System: A Recent Bioseparation Technology

Targeting Delivery System for Lactobacillus Plantarum Based on Functionalized Electrospun Nanofibers

Ultrasonic Thermal-Assisted Extraction of Phosvitin from Egg Yolk and Evaluation of Its Properties

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