Targeting Delivery System for Lactobacillus Plantarum Based on Functionalized Electrospun Nanofibers

Abstract

With the increased interest in information on gut microbes, people are realizing the benefits of probiotics to health, and new technologies to improve the viability of probiotics are still explored. However, most probiotics have poor resistance to adverse environments. In order to improve the viability of lactic acid bacteria, polylactic acid (PLA) nanofibers were prepared by coaxial electrospinning. The electrospinning voltage was 16 kV, and the distance between spinneret and collector was 15 cm. The feed rates of the shell and core solutions were 1.0 and 0.25 mL/h, respectively. The lactic acid bacteria were encapsulated in the coaxial electrospun nanofibers with PLA and fructooligosaccharides (FOS) as the shell materials. Scanning electron microscopy, transmission electron microscopy, and laser scanning confocal microscopy showed that lactic acid bacteria were encapsulated in the coaxial electrospun nanofibers successfully. The water contact angle test indicated that coaxial electrospun nanofiber films had good hydrophobicity. An in vitro simulated digestion test exhibited that the survival rate of lactic acid bacteria encapsulated in coaxial electrospun nanofiber films was more than 72%. This study proved that the viability of probiotics can be improved through encapsulation within coaxial electrospun PLA nanofibers and provided a novel approach for encapsulating bioactive substances.

New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization

“…Moreover, a strong fluorescence of these structures confirms that they are alive probiotic cells. A similar phenomenon has been reported by a previous studies (Mamvura et al, 2011 [ 26 ]; Yu et al, 2020 [ 27 ]).…”

“…Moreover, a strong fluorescence of these structures confirms that they are alive probiotic cells. A similar phenomenon has been reported by a previous studies (Mamvura et al, 2011 [26]; Yu et al, 2020 [27]). Furthermore, the electrospun PS nanofibers and PS/Alg material were characterized based on the SEM images (S-3400N, Hitachi, Tokyo, Japan) after gold coating (Figure 3).…”

“…The protection efficiency of L. plantarum 2675 cells by the produced membrane was investigated proving increased cell metabolic activity and growth under heat and UV radiation treatment in bath, MTT, and alamarBlue tests. Better thermal stability of the Lactobacillus cells encapsulated in the core-shell fiber mat (PVA) was noted by Feng et al (2020) [33], with the viability loss of 0.32 log(CFU mL −1 ) after 30 min in 70 • C. As suggested earlier by Feng et al (2018) [34], the high capacity of the produced PS nanofibers might be related to the shielding effect of the nanomaterial, thus physically inhibiting the hot diffusion into the material. The combination of polymer and hydrogel was successfully applied by Gensheimer et al (2011) [35].…”

Development of Antioxidant and Stable Conjugated Linoleic Acid Pickering Emulsion with Protein Nanofibers by Microwave-Assisted Self-Assembly

“…Confocal laser scanning microscopy (Leica Microsystems Inc., Heidelberg, Germany) was used to evaluate the microstructure of the emulsion derived from the previous experiment, with some modifications [27]. A total of 40 µL of the fluorescent dye Nile red (0.1%, w/v) and ThT (0.01%, w/v) were mixed thoroughly to make a fluorescent stain, and 1 mL of the sample was stained.…”

Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers

“…Different compounds can be incorporated into nanofibers, including small drug molecules, proteins, nucleic acids, and cells. Lactobacillus bacteria have also been incorporated into electrospun nanofibers, with L. plantarum being the most frequently used [24][25][26][27][28]. Other Lactobacillus species have also been electrospun using different polymers, namely, agrowaste amended with poly (vinyl alcohol) for Lactobacillus acidophilus [29], poly (vinyl alcohol) and sodium alginate for Lactobacillus rhamnosus [30], poly (vinyl alcohol) for Lactobacillus gasseri [31], and Eudragit L100 and sodium alginate for Lactobacillus paracasei [32].…”