The rapid growth in human population have great impact on environmental sustainability, health and well being of humankind. Due to the increase in density of population and social interaction of humanbeings chances of proliferation of infectious diseases and environmental pollution was increased. In particular increase in the hospital environmental pollution have attracted the interest of researchers across the globe to develop novel strategies and invent new health and hygiene-related products for the well being of mankind. In this regard, we are reporting an investigation on fabrication of cellulose-calcium hydroxide nanocomposites through a facile wet chemical-pad-dry-cure method. The physicochemical characteristics of the prepared nanocomposites were investigated using various analytical instruments including X-ray Diffraction, Atomic force microscopy, Scanning electron microscopy, Transmission electron microscopy, Selected area electron diffraction, Thermogravimetric analysis, Differential scanning calorimetry and Fourier transform infrared spectroscopy. Bactericidal activity of the fabricated nanocomposites was tested against four representative bacterias, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillius cereus. It is found that the prepared nanocomposites show bactericidal activity even after 25 wash cycles and can be used as a promising candidate to inhibit bacterial and nosocomial infections. We strongly believe that the facile method to fabricate ecofriendly and economically viable calcium hydroxide-cellulose nocomposites reported in this paper can provide a new direction in the development of nanotechnology based apparels for health care and biomedical applications.