Environmentally benign silver bio-nanomaterials as potent antioxidant, antibacterial, and antidiabetic agents: Green synthesis using Salacia oblonga root extract

Dugganaboyana, Guru Kumar; Mukunda, Chethan Kumar; Jain, Anisha; Kantharaju, Raghavendra Mandya; Nithya, Rani R.; Ninganna, Divya; Ahalliya, Rathi Muthaiyan; Shati, Ali A.; Alfaifi, Mohammad Y.; Elbehairi, Serag Eldin I.; Silina, Ekaterina; Stupin, Victor; Kanniappan, Gopalakrishnan Velliyur; Achar, Raghu Ram; Shivamallu, Chandan; Kollur, Shiva Prasad

doi:10.3389/fchem.2023.1114109

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…No other peak was observed within this range, which confirms the formation of pure AgNPs (Figure A). The observed value was within the range reported previously. ,, UV–vis spectrophotometry is a valuable technique for the characterization and monitoring the synthesis of AgNPs. In addition to light scattering, AgNPs have unique optical properties that enable them to interact with specific wavelength of light.…”

Section: Results and Discussionsupporting

confidence: 87%

“…Another study demonstrated significant antioxidant effects of AgNPs synthesized from root extract of Salacia oblonga. The activity was attributed to the adsorption of various phytochemical and functional groups on the surfaces of AgNPs . Our results further validate these studies.…”

Section: Results and Discussionsupporting

confidence: 82%

“…Due to its availability and cost-effectiveness, plant extracts have gained popularity for research purposes. A large number of plants such as Eugenia roxburghii, Salacia oblonga, Rubus ellipticus, Protium serratum, Teucrium apollinis, Pueraria tuberosa, Reynoutria japonica, and Ganoderma applanatum have been used for the synthesis of metal NPs with improved biological activities. However, the production of AgNPs from indigenous plant species such as A.…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesized Silver Nanoparticles Using Alnus nitida Leaf Extract as a Potential Antioxidant and Anticancer Agent

et al. 2023

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Biogenic synthesis of silver nanoparticles (AgNPs) using plant extracts is gaining attention as a substitute to the conventional physical and chemical synthesis methods. This study reports a facile, cost-effective, and ecofriendly synthesis of AgNPs using leaf extract of Alnus nitida (A. nitida) and their antioxidant and antiproliferative activities. The biosynthesized AgNPs were characterized using various analytical techniques including UV–visible spectroscopy, energy-dispersive spectrometry, scanning electron microscopy (SEM), Fourier transform infrared (FTIR), X-ray diffraction (XRD), and dynamic light scattering. The antioxidant and cytotoxic potential of the extract and AgNPs was evaluated using different in vitro models. The UV–vis analysis revealed a surface plasmon resonance peak of 400 nm corresponding to the synthesis of AgNPs. SEM analysis confirmed the formation of heterogeneously dispersed particles of nano size, while the XRD and FTIR spectra confirmed the crystallinity and existence of different functional groups that helped in capping and stability of AgNPs. The antioxidant activity of AgNPs and extract, studied by 1,1-diphenyl 2-picryl hydrazyl (DPPH), fluorescence recovery after photobleaching (FRAP), 2, 2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and H2O2 scavenging assays, showed a dose-dependent effect. The AgNPs at 1000 μg/mL significantly scavenged DPPH, FRAP, ABTS, and H2O2 by 66.45, 74.65, 78.81, and 72.56% with an average IC50 value of 33.31, 18.50, 16.46, and 15.65 μg/mL, respectively. The cytotoxic potential investigated by MTT assay revealed promising antiproliferative effects against different cancer cell lines. The IC50 values of AgNPs on MDA-MB-231, A549, and Hep-G2 cells were 14.88, 3.6, and 5.38 μg/mL, respectively. The results showed that AgNPs were more effective against lung and hepatocellular carcinoma. The selectivity index showed that AgNPs remained highly selective in retarding the growth of A549 and Hep-G2 cells as compared to normal cell lines HPAEpiC and HRPTEpiC. Overall, this study showed that biosynthesized AgNPs were associated with considerable antioxidant and cytotoxic effects. Our work suggests that A. nitida-mediated AgNPs should be evaluated further in order to develop safe and effective formulations for the treatment of different degenerative diseases.

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Section: Results and Discussionsupporting

confidence: 87%

Section: Results and Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

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Biosynthesized Silver Nanoparticles Using Alnus nitida Leaf Extract as a Potential Antioxidant and Anticancer Agent

et al. 2023

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“…The leaf extract–derived silver nanoparticles from S. mulbarica proved to be a better antibacterial agent against E. coli and B. subtilis and also possess the capability of ct-DNA damage via releasing of reactive oxygen species (Espenti et al 2020 ). In recent times, environmentally benign silver bio-nanomaterials have been synthesized from the root extracts of S. oblonga and served as potent antioxidant, antibacterial, and anti-diabetic agents (Dugganaboyana et al 2023 ). Recently, Sabeena et al ( 2023 ) performed a comparative study on chemical and green approaches for synthesis of nanocomposites from leaf extracts of S. reticulata and subsequently characterized for in vivo and in vitro biological properties.…”

Section: Introductionmentioning

confidence: 99%

Salacia spp.: recent insights on biotechnological interventions and future perspectives

Chavan,

Patil,

Patil

et al. 2024

Appl Microbiol Biotechnol

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The plants of the genus Salacia L. are the storehouse of several bioactive compounds, and are involved in treating human diseases and disorders. Hitherto, a number of reports have been published on in vitro biotechnology as well as microbial involvement in the improvement of Salacia spp. The present review provides comprehensive insights into biotechnological interventions such as tissue culture for plant propagation, in vitro cultures, and endophytic microbes for up-scaling the secondary metabolites and biological potential of Salacia spp. Other biotechnological interventions such as molecular markers and bio-nanomaterials for up-grading the prospective of Salacia spp. are also considered. The in vitro biotechnology of Salacia spp. is largely focused on plant regeneration, callus culture, cell suspension culture, somatic embryogenesis, and subsequent ex vitro establishment of the in vitro–raised plantlets. The compiled information on tissue cultural strategies, involvement of endophytes, molecular markers, and nanomaterials will assist the advanced research related to in vitro manipulation, domestication, and commercial cultivation of elite clones of Salacia spp. Moreover, the genetic diversity and other molecular-marker based assessments will aid in designing conservation policies as well as support upgrading and breeding initiatives for Salacia spp. Key points: • Salacia spp. plays a multifaceted role in human health and disease management. • Critical and updated assessment of tissue culture, endophytic microbes, metabolites, molecular markers, and bio-nanomaterials of Salacia spp. • Key shortcomings and future research directions for Salacia biotechnology.

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Antimicrobial Activity of Citrate-Coated Cerium Oxide Nanoparticles

Silina,

Ivanova,

Manturova

et al. 2024

Nanomaterials

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The purpose of this study was to investigate the antimicrobial activity of citrate-stabilized sols of cerium oxide nanoparticles at different concentrations via different microbiological methods and to compare the effect with the peroxidase activity of nanoceria for the subsequent development of a regeneration-stimulating medical and/or veterinary wound-healing product providing new types of antimicrobial action. The object of this study was cerium oxide nanoparticles synthesized from aqueous solutions of cerium (III) nitrate hexahydrate and citric acid (the size of the nanoparticles was 3–5 nm, and their aggregates were 60–130 nm). Nanoceria oxide sols with a wide range of concentrations (10−1–10−6 M) as well as powder (the dry substance) were used. Both bacterial and fungal strains (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris, Candida albicans, Aspergillus brasielensis) were used for the microbiological studies. The antimicrobial activity of nanoceria was investigated across a wide range of concentrations using three methods sequentially; the antimicrobial activity was studied by examining diffusion into agar, the serial dilution method was used to detect the minimum inhibitory and bactericidal concentrations, and, finally, gas chromatography with mass-selective detection was performed to study the inhibition of E. coli’s growth. To study the redox activity of different concentrations of nanocerium, we studied the intensity of chemiluminescence in the oxidation reaction of luminol in the presence of hydrogen peroxide. As a result of this study’s use of the agar diffusion and serial dilution methods followed by sowing, no significant evidence of antimicrobial activity was found. At the same time, in the current study of antimicrobial activity against E. coli strains using gas chromatography with mass spectrometry, the ability of nanoceria to significantly inhibit the growth and reproduction of microorganisms after 24 h and, in particular, after 48 h of incubation at a wide range of concentrations, 10−2–10−5 M (48–95% reduction in the number of microbes with a significant dose-dependent effect) was determined as the optimum concentration. A reliable redox activity of nanoceria coated with citrate was established, increasing in proportion to the concentration, confirming the oxidative mechanism of the action of nanoceria. Thus, nanoceria have a dose-dependent bacteriostatic effect, which is most pronounced at concentrations of 10−2–10−3 M. Unlike the effects of classical antiseptics, the effect was manifested from 2 days and increased during the observation. To study the antimicrobial activity of nanomaterials, it is advisable not to use classical qualitative and semi-quantitative methods; rather, the employment of more accurate quantitative methods is advised, in particular, gas chromatography–mass spectrometry, during several days of incubation.

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Environmentally benign silver bio-nanomaterials as potent antioxidant, antibacterial, and antidiabetic agents: Green synthesis using Salacia oblonga root extract

Cited by 3 publications

References 31 publications

Biosynthesized Silver Nanoparticles Using Alnus nitida Leaf Extract as a Potential Antioxidant and Anticancer Agent

Biosynthesized Silver Nanoparticles Using Alnus nitida Leaf Extract as a Potential Antioxidant and Anticancer Agent

Salacia spp.: recent insights on biotechnological interventions and future perspectives

Antimicrobial Activity of Citrate-Coated Cerium Oxide Nanoparticles

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