Environmental polycyclic aromatic hydrocarbon (PAH) exposure and DNA damage in Mexican children

Sánchez-Guerra, Marco; Pelallo-Martínez, Nadia Azenet; Dı́az-Barriga, Fernando; Rothenberg, Stephen J.; Hernández‐Cadena, Leticia; Faugeron, Sylvain; Oropeza-Hernández, Luis F.; Guaderrama-Díaz, Margarita; Quintanilla‐Vega, Betzabet

doi:10.1016/j.mrgentox.2011.12.006

Cited by 54 publications

(23 citation statements)

References 27 publications

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“…CYP1A1 genetic polymorphism was known to affect its enzyme activity, and variability in xenobiotic metabolism is associated with different susceptibility to given environmental pollutants (Bozina et al, 2009;Chen et al, 2008). A few recent studies have reported effects of the genetic polymorphism in the association of PAH exposure with DNA adducts, but not with pulmonary functions (Wang et al, 2008;Sánchez-Guerra et al, 2012;Park et al, 2006;Øvrebø et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

CYP1A1 genetic polymorphism and polycyclic aromatic hydrocarbons on pulmonary function in the elderly: Haplotype-based approach for gene–environment interaction

2013

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Section: Introductionmentioning

confidence: 99%

CYP1A1 genetic polymorphism and polycyclic aromatic hydrocarbons on pulmonary function in the elderly: Haplotype-based approach for gene–environment interaction

2013

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“…The environmental or occupational exposure to polycyclic aromatic hydrocarbons (PAH) can be monitored by the quantitation of metabolic intermediates of frequent and abundant chemicals in the mixture, such as 1-hydroxypyrene (1-OHP), the main pyrene metabolite (90%), with an average half-life of approximately 18-20 hours after pyrene inhalation (5). Urinary 1-OHP is routinely used as a biomarker in workers occupationally exposed to PAHs (6)(7)(8), tobacco smokers (9,10) and the general population exposed to airborne pollution (11)(12)(13) Requisites that a biological response should comply to be considered as a biomarker for health assessment (HPLC-FD), and/or gas chromatography coupled to mass spectrometry (GC-MS); its quantitation has shown a good correlation with PAH human exposure at both high and low environmental levels (5).…”

Section: Biomarkers Of Internal Dosementioning

confidence: 99%

“…The most common method for SNP analysis uses restriction endonucleases in cases where the polymorphism creates or destroys a specific nucleotide recognition sequence for a restriction enzyme (restriction fragment length polymorphism or RFLP). Other useful methods are real-time PCR using TaqMan probes (13,148,150), and primer extension-based methods (145,151,152) (table 3).…”

Section: Genetic Polymorphismsmentioning

confidence: 99%

Molecular biomarkers to assess health risks due to environmental contaminants exposure

Poblete-Naredo

Albores

2016

biomedica

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<p>Los biomarcadores, o bioindicadores, son herramientas métricas que, al compararse con los valores de referencia, permiten evaluar los riesgos y generar información objetiva que ayude a las autoridades a planificar estrategias efectivas, solucionar problemas de salud o ambientales y utilizar los recursos asignados de manera eficiente.<br />La evaluación de riesgos en salud es un ejercicio multidisciplinario y la biología molecular contribuye enormemente a estos estudios, dado que el genoma, el transcriptoma, el proteoma y el metaboloma pueden verse afectados por xenobióticos, lo que causa cambios cuya medición resulta útil en la adopción de decisiones. Dichos cambios pueden variar por la carga genética de cada individuo y su distribución polimorfa en las poblaciones expuestas se convierte en un factor esencial para una adecuada interpretación de resultados.<br />Por lo tanto, el objetivo del presente artículo fue hacer una revisión de los diferentes biomarcadores moleculares aplicables a la evaluación de riesgos para la salud provenientes de los contaminantes ambientales.</p>

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“…The urinary 1-hydroxypyrene (1-OHPyr) is a reliable and sensitive biomarker of exposure to the low-level occupational and environmental exposure to polycyclic aromatic hydrocarbons (PAHs). 1-OHPyr is one of the metabolites of PAHs, and can be used as a good biological indicator of exposure to the mixture of PAHs [3][4][5]. The urinary levels of the 1-OHPyr are commonly determined using the high pressure liquid chromatography (HPLC) equipped with a fluorescence detector, however, a limited number of literature studies has also referred to the UV-visible detector with the HPLC used for this purpose, whereas the quantified 1-OHPyr is also being carried out using a gas chromatography/mass spectrometry (GC/MS), which is the most preferable due to its high sensitivity and reliability, however, the procedures for sample cleanup for the GC/MS analysis are very laborious, limiting its usage [5].…”

Section: Introductionmentioning

confidence: 99%

Reverse phase high performance liquid chromatographic method development based on ultravioletvisible detector for the analysis of 1-hydroxypyrene (PAH biomarker) in human urine

Kamal¹,

Gulfraz²,

Anwar³

et al. 2015

Int J Occup Med Environ Health

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Objectives: 1-hydroxypyrene is an important biomarker of exposure to polycyclic aromatic hydrocarbons (PAHs), which appears in the urine of exposed human subjects. In developing countries, where advanced instruments are not available, the importance of this biomarker demands convenient and sensitive methods for determination purposes. This study aimed at developing a methodology to quantify 1-hydroxypyrene (a biomarker of PAHs exposure) based on the UV-visible detector in the reverse phase high pressure liquid chromatography (HPLC). Material and Methods: A 20 μl injection of sample was used for manual injection into the HPLC Shimadzu, equipped with the SPD-20 A UV-visible detector, the LC-20AT pump and the DGU-20A5 degasser. The C-18 column was used for the purpose of the analysis. Results: The method showed a good linearity (the range: R 2 = 0.979-0.989), and high detectability up to the nmol level. The average retention was 6.37, with the accuracy of 2%, and the percentage of recovery remained 108%. The overall performance of this method was comparable (in terms of detection sensitivity) and relatively better than previously reported studies using the HPLC system equipped with the UV-detector. Conclusions: This method is suitable and reliable for the detection/quantification of the 1-OHP in human urine samples, using the UV-detector, however, it is less sensitive as compared to the results of a florescence detector. with 5% HNO 3 , including subsequent washing with distilled water, and oven drying. The standards were prepared in the HPLC-grade acetonitrile (mmol/l). Serial dilutions of standards were carried out using the stock of 1 mmol/l in strength. The stock standard was diluted up to nmol/l. All the dilutions were prepared afresh each day before starting the calibration procedure. The stock standards were kept in a refrigerator in sterile glass vials to avoid solvent evaporation and contamination. Analytical proceduresAll the samples were filtered through the 0.02 μl syringe filters. A 20 μl injection of sample was used for manual injection into the HPLC Shimadzu equipped with the SPD-20 A UV-visible detector, the LC-20AT pump and the DGU-20A5 degasser. The C-18 column was used for the analysis purposes. HPLC optimization for the 1-OHPyr analysisThe HPLC conditions were optimized for the 1-OHPyr. The optimization was carried out by running a series of standard dilutions for a number of times, and the conditions were observed for 3 consecutive days to validate optimization. The optimum conditions for the 1-OHPyr were as follows: 20 μl injection, 250 bars pressure, a flow rate of 0.8 ml/minute, with a run time of 10 min, which was later reduced to 8 min. The mobile phase consisted of acetonitrile and the HPLC gradient grade water of 70:30. The column temperature was set at 30°C. Wavelength was set at 250 nm, and the average elution time was 6.371 min (Table 1). RESULTS AND DISCUSSIONThe analysis of the 1-OHPyr was carried using the UVvisible detector. In published literature studies, the florescence...

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Environmental polycyclic aromatic hydrocarbon (PAH) exposure and DNA damage in Mexican children

Cited by 54 publications

References 27 publications

CYP1A1 genetic polymorphism and polycyclic aromatic hydrocarbons on pulmonary function in the elderly: Haplotype-based approach for gene–environment interaction

CYP1A1 genetic polymorphism and polycyclic aromatic hydrocarbons on pulmonary function in the elderly: Haplotype-based approach for gene–environment interaction

Molecular biomarkers to assess health risks due to environmental contaminants exposure

Reverse phase high performance liquid chromatographic method development based on ultravioletvisible detector for the analysis of 1-hydroxypyrene (PAH biomarker) in human urine

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