Environmental levels, toxicity and human exposure to tributyltin (TBT)-contaminated marine environment. A review

Antízar-Ladislao, Blanca

doi:10.1016/j.envint.2007.09.005

Cited by 682 publications

(382 citation statements)

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“…The comparison of data from the literature is made difficult by the marked difference of organotin toxicity among taxonomic groups, the possibly related difference in sensitivity to TBT with a higher sensitivity of females than males (Hagger et al 2006;Horigushi et al 2006), the age-dependent organotin toxicity (Roberts et al 1987;Perina et al 2011), and the effect of interactions between TBT concentration and water temperature and salinity (Antizar-Ladislao 2008). In laboratory studies, butyltin associated with oxide (TBTO) or chloride (TBTCl) is normally used for experiments (Antizar-Ladislao 2008).…”

Section: Discussionmentioning

confidence: 99%

“…In laboratory studies, butyltin associated with oxide (TBTO) or chloride (TBTCl) is normally used for experiments (Antizar-Ladislao 2008). TBT concentration in sediments, as reported for several regions in the world, reaches up to 14 000 ng g −1 dw Sn (AntizarLadislao 2008).…”

Section: Discussionmentioning

confidence: 99%

“…The deleterious effects of TBT on marine bivalves have been widely reviewed (Alzieu 2000;Antizar-Ladislao 2008). For oysters, these include effects on reproduction, embryogenesis, larval growth, shell thickening, oxygen consumption, and adult survival, which can possibly lead to population decline.…”

Section: Discussionmentioning

confidence: 99%

“…Among marine pollutants, organotins, such as TBT, are highly toxic to a wide range of organisms (Inoue et al 2006a(Inoue et al , 2006bOhji et al 2005;Antizar-Ladislao 2008). The use of TBT in antifouling paints has been prohibited since 2008 (IMO, International Maritime Organization, www.imo.org).…”

Section: Introductionmentioning

confidence: 99%

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Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

Droguet

Devauchelle

Pennec

et al. 2012

Aquat. Living Resour.

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-European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10 −6 M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay, significantly decreased after two days of treatment with 10 −6 M TBT, and after six days with 10 −10 M TBT. The percentage of apoptotic cells, quantified by flow cytometry and YO-PRO r -1 iodide, a nucleic acid stain that only permeates cells that are beginning to undergo apoptosis, increased significantly in these cases. Moreover, intracellular concentration of Ca ++ had increased after 10 min of exposition to 10 −6 M, and could be associated with apoptotic processes. As patch clamp experiments showed that Ca ++ conductance was decreased, intracellular calcium increase could mainly be due to a release from internal stores. The decreases in beating rhythm could be explained by the decrease in adenosine triphosphate (ATP) production revealed by 31 P nuclear magnetic resonance (NMR) spectroscopy and confirmed by the increase of the K ATP channel conductance. The related hyperpolarization and the disturbances of the energetic metabolism were clearly related to the loss of the atrial cell contractility and viability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

Droguet

Devauchelle

Pennec

et al. 2012

Aquat. Living Resour.

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“…[1][2][3] In spite of the total worldwide ban proposed by the International Maritime Organization (IMO) in January 2008, these compounds are still found in different environmental compartments of aquatic systems. 4 Their toxic effects are observed in many species because tributyltin (TBT) can induce an endocrine disruption process known as imposex.…”

Section: Introductionmentioning

confidence: 99%

Tributyltin in crustacean tissues: analytical performance and validation of method

Cristale

Santos

Sant’Anna

et al. 2012

J. Braz. Chem. Soc.

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O ermitão Clibanarius vittatus é um organismo típico de regiões intertidais estuarinas sendo considerado um possível bioindicador da presença de tributilestanho (TBT) nesses ambientes. Por esta razão, este estudo apresenta o desempenho analítico e a validação do método para quantificação de TBT em tecidos de C. vittatus por cromatografia gasosa com detector fotométrico de chama pulsante (GC-PFPD), após extração com solvente apolar (tolueno) e derivatização com reagente de Grignard. Os limites de detecção do método (LOD) foram 2,0 e 2,8 ng g -1 para TBT e DBT (dibutil estanho), respectivamente, e seus limites de quantificação (LOQ) 6,6 e 8,9 ng g -1 para TBT e DBT, respectivamente. O método foi aplicado em amostras do Estuário de Santos, Estado de São Paulo, Brasil. As concentrações de TBT e DBT variaram de 26,7-175,0 ng g -1 a 46,2-156,0 ng g -1 , respectivamente. As concentrações encontradas saopreocupantes uma vez que efeitos tóxicos (tais como a alteração endócrina) têm sido relatados em outros organismos, mesmo em níveis inferiores aos registrados no presente estudo.The hermit crab Clibanarius vittatus is a typical organism from intertidal regions being considered as a good bioindicator of tributyltin presence at these environments. Thus this study presents the analytical performance and validation method for TBT quantification in tissues of C. vittatus by gas chromatography with pulsed flame photometric detector (GC-PFPD) after extraction with an apolar solvent (toluene) and Grignard derivatization. The limits of detection of the method (LOD) were 2.0 and 2.8 ng g -1 for TBT and DBT (dibutyltin), respectively, and its limits of quantification (LOQ) were 6.6 and 8.9 ng g -1 for TBT and DBT, respectively. The method was applied to samples from Santos Estuary, São Paulo State, Brazil. TBT and DBT concentrations ranged from 26.7 to 175.0 ng g -1 and from 46.2 to 156.0 ng g -1 , respectively. These concentrations are worrisome since toxic effects (such as endocrine disruption) have been reported for other organisms even under lower levels of registred at this study.Keywords: crustaceans, organotin, tributyltin (TBT), gas chromatography IntroductionButyltin compounds (BTs), members of the class of organometallic contaminants, are found in coastal regions worldwide because they were used in antifouling paints for many years. [1][2][3] In spite of the total worldwide ban proposed by the International Maritime Organization (IMO) in January 2008, these compounds are still found in different environmental compartments of aquatic systems. 4 Their toxic effects are observed in many species because tributyltin (TBT) can induce an endocrine disruption process known as imposex. 5 This effect in mollusks has been well documented. [6][7][8] Other effects caused by TBT include shell malformation in the Pacific oyster Crassostrea gigas, suppressed Tributyltin in Crustacean Tissues: Analytical Performance and Validation of Method J. Braz. Chem. Soc. 40 growth in the centric diatoms Skeletonema costatum and Thalassiosira pse...

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Hyphenated Methods for Speciation Analysis

Michalski¹

2019

Encyclopedia of Analytical Chemistry

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Speciation analysis, understood as research into various element forms, is gaining importance in environment protection; biogeochemical cycles of chemical compounds; determination of toxicity and ecotoxicity of selected elements; quality control of food, medicines, and pharmaceutical products; examination of occupational exposure and clinical analysis. The hyphenated methods, in which separation method is coupled with multidimensional detectors, have become very useful in such analyses. The main advantages of those methods consist in extremely low detection and quantification limits, insignificant interference influence as well as high precision and repeatability of the determinations. The following article describes the role and importance of hyphenated methods in speciation analyses of selected elements.

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Environmental levels, toxicity and human exposure to tributyltin (TBT)-contaminated marine environment. A review

Cited by 682 publications

References 182 publications

Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

Tributyltin in crustacean tissues: analytical performance and validation of method

Hyphenated Methods for Speciation Analysis

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