Environmental influences on adventitious rooting in cuttings of non-woody species

Andersen, A. Skytt

doi:10.1007/978-94-009-4358-2_7

Cited by 34 publications

(27 citation statements)

References 73 publications

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“…O enraizamento pode ter sido promovido por um balanço endógeno em favor da auxina ou pela presença de cálcio, boro e zinco no meio de cultura MS, já que esses nutrientes são conhecidos como rizogênicos (DE FOSSARD et al, 1977;ANDERSEN, 1986). Diversas espécies, principalmente as herbáceas, enraízam na presença de níveis muito reduzidos de auxina ou em meio básico sem reguladores de crescimento.…”

Section: Resultsunclassified

Micropropagação de singônio.

Schwertner¹,

Zaffari²

2003

RBHO

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A propagação vegetativa de Syngonium podophyllum Schott (Araceae) em campo torna-se difícil para os produtores que buscam obter mudas de qualidade e em larga escala, em vista de problemas fitossanitários. Utilizaram-se gemas laterais da axila foliar de S. podophyllum para micropropagar essa espécie ornamental e, na fase de estabelecimento, submeteram-se os explantes a dois procedimentos de desinfestação: o primeiro consistiu em uma pré-assepsia em bancada de laboratório, com imersão das gemas em hipoclorito de sódio a 1%, durante 15 minutos, e de uma assepsia em câmara de fluxo laminar, com imersão em etanol 70% e em hipoclorito de sódio a 1%, por 3 e 30 minutos respectivamente; o segundo procedimento consistiu na assepsia apenas em câmara de fluxo laminar. Os explantes foram inoculados em meio MS e MS adicionado de ácido 3-indolacético (AIA) e 6-benzilaminopurina (BAP), ambos a 1,0 mg.L -1 . Na fase de proliferação, submeteram-se os explantes a concentrações de 1,0; 2,5 e 4,0 mg.L -1 de BAP, adicionadas ao meio MS, transferindo-se os brotos obtidos para frascos com meio MS para o crescimento e desenvolvimento de plântulas. A exposição das gemas laterais axilares a uma pré-assepsia em bancada e posterior assepsia em câmara de fluxo laminar foi mais eficiente na desinfestação dos explantes, com 100 % de sobrevivência. A adição dos reguladores de crescimento, AIA e BAP, ambos a 1,0 mg.L -1 , no meio MS, promoveu maior desenvolvimento das plântulas durante o estabelecimento in vitro da cultura. Concentrações elevadas de BAP no meio de proliferação não induziram um aumento significativo no número médio de brotos por explante; entretanto, resultaram numa redução significativa do número médio de folhas e raízes. Os explantes provenientes dos subcultivos de proliferação do tratamento com 1,0 mg.L -1 de BAP, quando cultivados em meio MS, isento de reguladores de crescimento, apresentaram maior desenvolvimento de plântulas. Palavras-chave:Syngonium podophyllum Schott (Araceae), desinfestação, micropropagação, reguladores de crescimento. ABSTRACT Micropropagation of Syngonium podophyllumFarmers who search for large scale and quality seedlings, have difficulty in propagating Syngonium podophyllum Schott (Araceae) at field due to plant health problems. Therefore, lateral buds of S. podophyllum leaf axils were used to micropropagate this ornamental species. At establishment stage, they were submitted to two disinfection procedures and further by inoculated in MS medium with and/or without the addition of 3-indolacetic acid (IAA) and 6-benzilaminopurine (BAP) growth regulators. At development stage, the explants were submitted to different BAP concentrations added to MS medium. The buds obtained were transferred to flasks containing MS for plant development. The exposition of lateral axilary buds to a primary asepsis in laboratory followed by a secondary asepsis in laminar flow bench, proved to be more efficient in disinfecting the explants, with 100% survival rate. The addition of growth regulators, IAA and BAP both at 1.0 m...

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Section: Resultsunclassified

Micropropagação de singônio.

Schwertner¹,

Zaffari²

2003

RBHO

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“…The outdoor mini-hedges thus produced were, however, at the mercy of the climate, and problems of adequate maintenance of nutritional status and control of leaf disease, especially during winter, were often encountered. Indoor mini-hedging systems have technical and economic advantages compared with normal outdoor clonal hedging, including higher productivity, lower labor demands, low consumption of chemicals, and also on rooting success (Andersen 1986;Haissig 1986;Read 1987;Hartmann et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Optimization of seasonality and mother plant nutrition for vegetative propagation of Pinus pinaster Ait

et al. 2012

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Due to the high economic importance of Pinus pinaster Ait., there is considerable interest in developing, improving and extending the use of its families for mass clonal propagation and in breeding programmes. In the current study, we evaluated shoot growth, rooting ability and mini-cuttings production of P. pinaster in response to nitrogen fertilization and seasons. We compared eight half-sib families of P. pinaster from Asturias and Galicia (Northern Iberian Peninsula), searching for useful parameters and growing conditions to be included in a mass propagation program for clonal family forestry. We fertilized P. pinaster seedling mother plants kept in a greenhouse with three levels of nitrogen: high (HN), medium (MN) and low (LN) to evaluate rooting ability of minicuttings. In addition, we evaluated the maximal potential production of rooted mini-cuttings considering nine cycles of propagation over 1 year, also using three levels of nitrogen. The HN treatment significantly influenced the rooting process, with length, area and volume of roots all being positively affected. Spring was the most favourable season for mini-cuttings in the HN treatment. This study provides valuable new information to optimize the clonal propagation protocol for P. pinaster and shows that the mini-cuttings technique has great potential in mass scale cloning, providing high quality sprout production and well-formed new plants.

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“…Ex vitro rooted plantlets are usually more suited for transplantation as compared to in vitro rooted plantlets (Andersen 1986). The root system developed ex vitro was different from the root system developed during in vitro rooting practice.…”

Section: Discussionmentioning

confidence: 99%

Enhanced micropropagation protocol of ex vitro rooting of a commercially important crop plant Simmondsia chinensis (Link) Schneider

Singh¹,

Agarwal²

2016

J. For. Sci.

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A micropropagation protocol was developed by further improvement of prevailing methods using proven germplasm for ex vitro rooting and addressed the effect of the number of subcultures on the rooting ability of shoots. A comparative study was done between in vitro rooting method and ex vitro rooting method. Using the ex vitro rooting method a plantlet could be produced in 135 days, which was in a shorter time compared to the in vitro rooting method -180 days. The best axillary shoot bud induction was observed on Murashige and Skoog (MS) medium supplemented with 4.6 µM thidiazuron (TDZ) with 5 shoot buds per explant. In the shoot cluster, which was subcultured on MS medium supplemented with 2.3 µM TDZ, the rate of shoot multiplication increased in the 3 rd subculture. The maximum mean number of shoots per explant (20) was obtained at the 3 rd subculture on the same medium. Shoots were harvested at the 1 st , 3 rd and 5 th subculture and pulse treated for root induction. The highest rooting (95%) was achieved from the 3 rd subculture onwards with pulse treated shoots for fifteen days. The rooted plants could be established in a greenhouse with 99% survival. Ex vitro rooting is a promising method to reduce the time for plant generation. The resultant plantlets well established in pots and fruiting was observed within a year.

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Environmental influences on adventitious rooting in cuttings of non-woody species

Cited by 34 publications

References 73 publications

Micropropagação de singônio.

Micropropagação de singônio.

Optimization of seasonality and mother plant nutrition for vegetative propagation of Pinus pinaster Ait

Enhanced micropropagation protocol of ex vitro rooting of a commercially important crop plant Simmondsia chinensis (Link) Schneider

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