its fruit and oil has been harvested and processed remains a potential adjuvant for this course. This is so because studies revealed that it is rich in phytochemicals and has several medicinal values. 11,12 However, recent studies by Achuba 13 revealed that incorporation of dried oil palm leave powder in crude oil-polluted meals significantly prevented the negative outcomes accompanying crude oil toxicity. This investigation was aimed at assessment of different regimen of oil palm leaf extracts against crude oil-adulterated feed mediated nephrotoxicity.
Materials and Methods
Collection of palm leaves, crude oil and reagentsHarvest of Elaeis guineensis leaves were done at Ovwor Mixed Secondary School in Ughelli, Delta State, Nigeria on May 11, 2016. This was immediately rinsed with water to remove all forms of dirt and taken to the laboratory. The leaves were identified at Forestry Research Institute of Nigeria, Ibadan where voucher number, F101173 was assigned. The crude oil, Escravos blend type, was obtained from Warri Refining and Petrochemical Company, Warri, Nigeria. The reagents used were analytical grade.
Experimental animalsMale albino rats (One hundred and twenty) were sourced from Faculty of Basic Medical sciences, Delta State University, Nigeria. The weights of the rats were taken and it ranged from 148 to 180 g. The experimental rats were maintained on grower's mash and exposed to laboratory environment for two weeks. This is to enable them acclimatize to the feed and the new environment. Ethical approval, with the number REC/FOS/19/01, for the use the animals was granted by the ethical committee,