Effects of Crude Oil contaminated Water on the reproductive system of Female Wistar Rats

Abstract

The rising level of infertility in the females have been associated with environmental pollutants and one such pollutants is crude oil. Crude oil pollution occurs in the Niger-Delta region of Nigeria and interaction with this toxicant by both humans and animals may adversely the female reproductive system. This study was designed to investigate the effect of crude oil contaminated water on reproductive functions in female Wistar rats. Fifteen (15) female rats weighing between 140g-230g were used for this study and the rats were divided into three (3) groups (1, 2 and 3) and each group had five rats. Group 1 was the control group while group 2 and 3 were the test groups receiving 2.5mls and 5mls of crude oil contaminated water twice daily for four weeks. The stages of Oestrus cycle of the rats were checked daily. The rats were sacrificed after four weeks and the blood collected were used for hormonal assays (Follicle stimulating hormone (FSH), Luteinizing hormone (LH), Testosterone).The uterus and ovaries were used for histology (Haematoxylin and Eosin staining), Superoxide dismutase (SOD) activity and Malondialdehyde (MDA) concentration. The results were analyzed using GraphPad Prism software for windows and data were expressed as mean±standard error of mean. Statistical significance was accepted at p<0.05.