Environmental, genotypic, and ploidy effects on endopolyploidization within a genotype of <i>Solanum phureja</i> and its derivatives

Owen, Henry; Veilleux, Richard E.; Levy, David A.; Ochs, D. L.

doi:10.1139/g88-085

Cited by 30 publications

(12 citation statements)

References 20 publications

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“…This multiploidy described in Arabidopsis (Arumuganathan and Earle 1991), or polysomaty described in potato (Sree Ramulu and Dijkhuis 1986) has been recognized as being under developmental control and species-, genotype-and tissuespecific (Arumuganathan and Earle 1991;Ochatt 2008;Sree Ramulu and Dijkhuis 1986). Histograms reported in the earlier FC studies in potato or its related species showed also multiploidy in leaf samples (Johnson et al 2001;Owen et al 1988;Rokka et al 1995;Valkonen et al 1994). Thus, it could be a general occurrence in potato.…”

Section: Resultsmentioning

confidence: 91%

Efficient Chromosome Number Estimation Using Flow Cytometry in the Backcross of Solanum demissum (2n = 6x = 72) to S. tuberosum (2n = 4x = 48)

Ono

Hosaka

2010

Am. J. Pot Res

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A Mexican hexaploid wild species Solanum demissum has been extensively used by backcrossing to tetraploid cultivars in potato breeding. Because of practical difficulty in chromosome counting of the aneuploid progeny, selection in backcross generations has been limited to agronomic performances. We employed flow cytometry (FC), which could distinguish plants with the chromosome number difference less than 1.4 with 95% probability. Thus, FC is useful in selecting near-tetraploid level plants in a short period and at earlier generations.Resumen Una especie Silvestre Mexicana hexaploide, Solanum demissum, ha sido utilizada extensivamente mediante retrocruzas con variedades tetraploides en el mejoramiento de la papa. Debido a dificultades prácticas en el conteo cromosómico de la progenie aneuploide , la selección de las generaciones en las retrocruzas se ha limitado a los comportamientos agronómicos. Nosotros empleamos citometría de flujo (FC), que puede distinguir plantas con diferencia en el número cromosómico menor a 1.4 con un 95% de probabilidad. Así, FC es útil en la selección de plantas con un nivel cercano al tetraploide en un período corto y en generaciones más tempranas.

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Section: Resultsmentioning

confidence: 91%

Efficient Chromosome Number Estimation Using Flow Cytometry in the Backcross of Solanum demissum (2n = 6x = 72) to S. tuberosum (2n = 4x = 48)

Ono

Hosaka

2010

Am. J. Pot Res

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“…Both clones can be traced to the diverse, adapted population of S. phureja-S. stenotomum developed by Haynes (1972). AM3-8, the doubled monoploid (2n = 2x = 24), was produced by chromosome doubling during shoot regeneration from leaf discs of a monoploid, AM3 (2n = x = 12) (Owen et al 1988). It transmits anther culture competence to its progeny (Singsit & Veilleux 1989), but its protoplasts do not divide after isolation and culture (Cheng & Veilleux 1991).…”

Section: Methodsmentioning

confidence: 99%

Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and corrleations among them

Taylor

Veilleux

1992

Plant Cell Tiss Organ Cult

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Competence for leaf disc regeneration, anther culture, and protoplast culture was examined in the parental, F1, and F 2 generations of a population of the diploid, cultivated, primitive potato, S. phureja (2n = 2x = 24). The parental pair consisted of AM3-8, an anther culture derived homozygous diploid, and NBP2, a heterozygous, field selected line. AM3-8 produced embryos in anther culture, and shoots on cultured leaf discs, but its cells did not divide after protoplast isolation. Cells of NBP2 divided to form calli and shoots in protoplast culture, but the clone did not respond to anther culture or leaf disc regeneration. All the individual plants in the F 1 generation were responsive to both anther and protoplast culture; however, there was segregation for the ability to regenerate shoots from leaf discs. The F 2 population, the result of a sib-cross, segregated for all three tissue culture competencies. Segregation data fit a one gene model for anther culture competence with the homozygous dominant genotype expressing the highest response, the heterozygote resulting in a marginal response, and the homozygous recessive resulting in no response. A two-gene model applied to the protoplast culture data, with a dominant allele at both loci required for division to occur after protoplast isolation. Leaf disc regeneration data could only be explained by a two gene model with recessive alleles at each locus required for the highest response, a dominant allele at either of the loci resulting in a marginal response, and dominant alleles at both loci resulting in no response. No significant correlation was found among these traits, implying three separate genetic mechanisms which segregate independently.

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“…Androgenic monoploids were derived through anther culture of PP5 and BARD 1-3 (Snider and Veilleux 1994). The monoploids were verified by flow cytometry (Owen et al 1988) and maintained in vitro.…”

Section: Plant Materialsmentioning

confidence: 99%

“…The plantlets were grown and analyzed in a randomized complete block design with three replications. DNA content was estimated via flow cytometry as described by Owen et al (1988) using a Coulter Epics XL flow cytometer (Coulter International Corporation, Miami, Florida.). Data were collected on the frequencies of nuclei comprising the 1×, 2×, and 4× peaks and the peak positive values (the channel number where the highest frequency of cells was found in each peak).…”

Section: Nuclear Dna Comparisonmentioning

confidence: 99%

Selection inherent in monoploid derivation mechanisms for potato

Lough¹,

Varrieur²,

Veilleux³

2001

Theor Appl Genet

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Monoploid potato (2n=1×=12) can be derived either paternally through anther or microspore culture (androgenesis) or maternally through crossing with a haploid-inducing pollinator (gynogenesis). Androgenic and gynogenic monoploid populations, derived from each of two Solanum phureja clones, were compared in both greenhouse and field studies, and their frequency of cells at the 1x, 2x and 4x DNA contents was estimated by flow cytometry of in vitro plantlets. In 15 of 17 comparisons of morphological data (plant height, number of main stems, vigor, leaf length and width, internode length, tuber number and tuber weight) where a significant difference was found due to derivation, the androgenic monoploids were agronomically superior to their gynogenic counterparts (13-18% greater leaf size, double to triple the total tuber yield). Only plant height was significantly greater (26-27%) in the gynogenic monoploids. Flow cytometry revealed that the gynogenic monoploids retained 5% more cells at the monoploid level, whereas androgenic monoploids exhibited 27% more endopolyploid cells at the tetraploid level.

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Environmental, genotypic, and ploidy effects on endopolyploidization within a genotype of Solanum phureja and its derivatives

Cited by 30 publications

References 20 publications

Efficient Chromosome Number Estimation Using Flow Cytometry in the Backcross of Solanum demissum (2n = 6x = 72) to S. tuberosum (2n = 4x = 48)

Efficient Chromosome Number Estimation Using Flow Cytometry in the Backcross of Solanum demissum (2n = 6x = 72) to S. tuberosum (2n = 4x = 48)

Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and corrleations among them

Selection inherent in monoploid derivation mechanisms for potato

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