Environmental DNA (eDNA) Detection Probability Is Influenced by Seasonal Activity of Organisms

Souza, Lesley S. de; Godwin, James C.; Renshaw, Mark A.; Larson, Eric R.

doi:10.1371/journal.pone.0165273

Cited by 131 publications

(142 citation statements)

References 51 publications

Supporting

Mentioning

131

Contrasting

Order By: Relevance

“…eDNA is rapidly moving from proof of concept to actual implementation for purposes like surveillance for the early arrival of new or spreading invasive species (e.g., Jerde et al, 2013;Egan et al, 2015) or documenting distributional or population trends for imperiled species (e.g., de Souza et al, 2016). Here we report the first application of eDNA to test for major range expansions of invasive crayfishes into new regions where they were not previously known to occur, but where climatic conditions are anticipated to be suitable for their population establishment and spread Morehouse & Tobler, 2013).…”

Section: Discussionmentioning

confidence: 99%

Environmental DNA (eDNA) detects the invasive crayfishes Orconectes rusticus and Pacifastacus leniusculus in large lakes of North America

et al. 2017

Self Cite

View full text Add to dashboard Cite

We report results of a study that made reciprocal comparisons of environmental DNA (eDNA) assays for two major invasive crayfishes between their disparate invasive ranges in North America. Specifically, we tested for range expansions of the signal crayfish Pacifastacus leniusculus (Dana, 1852) into the Laurentian Great Lakes region known to be invaded by the rusty crayfish Orconectes rusticus (Girard, 1852), as well as for the invasion of O. rusticus into large lakes of California and Nevada, US known to be invaded by P. leniusculus. We compared eDNA detections to historic localities for O. rusticus within the Great Lakes, and to recent sampling for presence/absence and relative abundance of P. leniusculus in California and Nevada via overnight sets of baited traps. We successfully detected O. rusticus eDNA at six sites from the Great Lakes and P. leniusculus from six of seven lakes where it was known to occur in California and Nevada, but did not detect any range expansions by either species across the North American continent. eDNA appears suitable to detect benthic arthropods from exceptionally large lakes, and will likely be useful in applications for monitoring of new biological invasions into these and other freshwater and marine habitats.

show abstract

Section: Discussionmentioning

confidence: 99%

Environmental DNA (eDNA) detects the invasive crayfishes Orconectes rusticus and Pacifastacus leniusculus in large lakes of North America

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Water samples were collected monthly May-September 2018. Sampling during spawning is likely to increase detectability of eDNA owing to increased activity and surge of gametes in the environment (Spear et al 2014, de Souza et al 2016, Erickson et al 2017, Sepulveda et al 2019). Dreissenid mussel spawning temperatures are 9-24°C, and spawning is maximized at water temperatures ≥18-20°C (reviewed in Garton et al 2013).…”

Section: Site Selectionmentioning

confidence: 99%

Adding invasive species biosurveillance to the U.S. Geological Survey streamgage network

et al. 2019

View full text Add to dashboard Cite

The costs of invasive species in the United States alone are estimated to exceed US$100 billion per year, so a critical tactic in minimizing the costs of invasive species is the development of effective, early-detection systems. To this end, we evaluated the efficacy of adding environmental (e)DNA surveillance to the U.S. Geological Survey (USGS) streamgage network, which consists of >8200 streamgages nationwide systemically visited by USGS hydrologic technicians. Incorporating strategic eDNA sample collection during routine streamgage visits could provide early-detection surveillance of aquatic invasive species with minimal additional cost. For this evaluation, USGS hydrologic technicians collected monthly eDNA water samples, May-September 2018, from streamgages downstream of reservoirs in the Columbia River Basin thought to be vulnerable to invasive dreissenid mussel (Dreissenidae spp.) establishment. We tested water samples for dreissenid mussel DNA and also for kokanee (Oncorhynchus nerka) and yellow perch (Perca flavescens) DNA; the two fishes were used to assess if streamgages are adequately located to provide early-detection eDNA surveillance of taxa known to be present in upstream reservoirs. No Columbia River Basin streamgage samples met our criteria for being scored as positive for dreissenid DNA. We did detect kokanee and yellow perch DNA at all streamgages downstream of reservoirs where these species are known to occur. Field collection, laboratory analyses, and personnel time required for collection of four eDNA samples at a streamgage site cost US$500-US$600 (net). Given these results, incorporating eDNA biosurveillance into routine streamgage visits might decrease costs associated with an invasion since early detection maximizes the potential for eradication, containment, and mitigation.

show abstract

“…Our results provide further support for the negative influence water turbidity has on eDNA detection probability ( Figure 3). Thus, seasonal activity patterns of resident study organisms should be carefully considered in future studies to either maximize or account for differences in eDNA detection (de Souza et al, 2016). Initial eDNA detection could have been slightly lower due to higher flows and increased turbidity during spring when occasional flooding occurs.…”

Section: Environmental Factors Affecting Edna Detectionmentioning

confidence: 99%

Environmental DNA improves Eastern Hellbender (Cryptobranchus alleganiensis alleganiensis) detection over conventional sampling methods

et al. 2019

View full text Add to dashboard Cite

Effective conservation planning relies on accurate species detection. However, conventional sampling methods used for detecting rare and cryptic aquatic species suffer from low probabilities of detection. Environmental DNA (eDNA) has emerged as an innovative and powerful sampling tool for detecting aquatic species, with previous studies suggesting a detection advantage over conventional sampling. However, comparative studies often fail to consider the appropriate sampling frameworks to adequately compare sampling methodologies and account for the influence of environmental variables on eDNA detection probabilities. In this study, we paired two detection methods (eDNA and physical sampling) at 22 sites in West Virginia, USA, to compare the probability of detecting a cryptic, elusive, and imperiled species of giant salamander, the Eastern Hellbender (Cryptobranchus alleganiensis alleganiensis). We used a multimethod occupancy modeling framework to compare method-specific detection probabilities using a suite of predictor variables based on environmental conditions thought to influence hellbender detection. We detected hellbenders at 19/22 sites using eDNA and at 13/22 sites using physical sampling methods. The best supported model indicated that detection probability for eDNA (0.84 ± 0.06) was three times higher than conventional methods (0.28 ± 0.07). Water turbidity was the best predictor of hellbender detection and negatively impacted our ability to detect eDNA. We failed to detect an association between eDNA concentration and hellbender catch per unit effort. Our study supports previous findings that suggest eDNA sampling methods greatly increase the probability of detecting aquatic species. However, with little known about the influence of environmental variables on eDNA detection, our results highlight the negative influence turbidity and other physiochemical factors have on eDNA detection and suggest that further research on eDNA detection in turbid environments is needed. | 87 WINELAND Et AL.

show abstract

Environmental DNA (eDNA) Detection Probability Is Influenced by Seasonal Activity of Organisms

Cited by 131 publications

References 51 publications

Environmental DNA (eDNA) detects the invasive crayfishes Orconectes rusticus and Pacifastacus leniusculus in large lakes of North America

Environmental DNA (eDNA) detects the invasive crayfishes Orconectes rusticus and Pacifastacus leniusculus in large lakes of North America

Adding invasive species biosurveillance to the U.S. Geological Survey streamgage network

Environmental DNA improves Eastern Hellbender (Cryptobranchus alleganiensis alleganiensis) detection over conventional sampling methods

Contact Info

Product

Resources

About