Environmental chemical mutagens and genetic risks: Lessons from radiation genetics

Sankaranarayanan, K.

doi:10.1002/(sici)1098-2280(1996)28:2<65::aid-em1>3.0.co;2-d

Cited by 6 publications

(2 citation statements)

References 16 publications

(5 reference statements)

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“…A variety of chemical and environmental mutagens result in chromosomal deletions and rearrangements (Sankaranarayanan, 1996; Grant & Owens, 1998). Terminal deletions are an extreme form; one or both arms of a chromosome are deleted, leaving the centromeric region and flanking regions of variable size.…”

Section: Introductionmentioning

confidence: 99%

Meiotic and mitotic instability of two EMS-produced centric fragments in the haplodiploid wasp Nasonia vitripennis

Perrot‐Minnot

Werren

2001

Heredity

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Terminal deletions that result in chromosomal fragments with centromeres (centric fragments) are relatively easy to generate and study in the haplodiploid insect Nasonia. We investigated the transmission stability of two chromosomal fragments generated by chemical mutagenesis. Visible mutations at the R locus (peach-233 and St-DR) and a linked body-colour mutant (purple) were used to track transmission of the centric fragments (which lack the purple locus and are wild-type at the R locus). Transmission rates in meiotic oogenesis were low (medians 0.15±0.18) and comparable to previous data on centric fragments in this species. The homologous chromosome genetic background strongly aected meiotic stability of one centric fragment (CF2) but not the other (CF1). Speci®cally, in peach/scarlet R locus heterozygous females, CF2 showed a normal segregation proportion with the chromosome bearing the scarlet allele (0.16), but near complete failure to segregate with peach (0.0002). Data show that this is due to loss of CF2 in eggs receiving peach, rather than to preferential segregation of CF2 with scarlet or mortality of CF2-bearing males. CF1 shows typical segregation ratios with both chromosomes. We hypothesize that deletions (or rearrangements) associated with the peach-233 mutant inhibit proper pairing and segregation of CF2. Consistent with the model, CF2 did segregate with chromosomes that had undergone recombination between peach and purple (a bodycolour mutation 10 cM M from peach), indicating that the domains inhibiting segregation are closely linked to peach. Mitotic instability also diered between the two fragments; reduced mitotic stability may relate to absence of telomeres on these centric fragments. Given the relative ease of generating and tracking terminal deletions in Nasonia, we propose this as a good system for studying mitotic and meiotic stability of centric fragments. Finally, results are discussed in relation to the evolution of B chromosomes from centric fragments.Keywords: B-chromosome, centric fragment, EMS, haplodiploid, mitotic instability, mutagen. IntroductionA variety of chemical and environmental mutagens result in chromosomal deletions and rearrangements (Sankaranarayanan, 1996; Grant & Owens, 1998). Terminal deletions are an extreme form; one or both arms of a chromosome are deleted, leaving the centromeric region and¯anking regions of variable size. Such largescale deletions are typically lethal or highly detrimental, because they are often associated with aneuploidy or dosage compensation eects, including partial trisomy of pericentric regions in humans (Stankiewicz et al., 2000). Under some circumstances, major terminal deletions can result in a chromosome containing only the centromere and a relatively small¯anking region. These are referred to as`centric fragments'. In humans, they are referred to as`marker chromosomes' and are implicated in the aetiology of abnormal phenotypes (Li et al., 2000; Stankiewicz et al., 2000). Terminal deletions also typically lack telomeres on the dele...

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Section: Introductionmentioning

confidence: 99%

Meiotic and mitotic instability of two EMS-produced centric fragments in the haplodiploid wasp Nasonia vitripennis

Perrot‐Minnot

Werren

2001

Heredity

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“…A variety of sources can induce stress to bacterial cells, e.g., radiation (Sankaranarayanan, 1996), heat (Roncarati and Scarlato, 2017), salt (Egamberdieva et al, 2017), chemical mutagens (Abraham et al, 2006), carbon starvation (Handtke et al, 2018), and metabolites (Vainio et al, 1981;Kulling et al, 2002). To survive and thrive in these extreme conditions, microbes have a repertoire of genes that can be activated or silenced responding to stress (van der Veen and Abee, 2011).…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Comparative Proteomic Analysis of Acinetobacter baylyi ADP1 Under DNA Damage in Relation to Different Carbon Sources

Jiang

Xing

et al. 2020

Front. Microbiol.

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DNA damage response allows microorganisms to repair or bypass DNA damage and maintain the genome integrity. It has attracted increasing attention but the underlying influential factors affecting DNA damage response are still unclear. In this work, isobaric tags for relative and absolute quantification (iTRAQ)-based proteomic analysis was used to investigate the influence of carbon sources on the translational response of Acinetobacter baylyi ADP1 to DNA damage. After cultivating in a nutrient-rich medium (LB) and defined media supplemented with four different carbon sources (acetate, citrate, pyruvate, and succinate), a total of 2807 proteins were identified. Among them, 84 proteins involved in stress response were significantly altered, indicating the strong influence of carbon source on the response of A. baylyi ADP1 to DNA damage and other stresses. As the first study on the comparative global proteomic changes in A. baylyi ADP1 under DNA damage across nutritional environments, our findings revealed that DNA damage response in A. baylyi ADP1 at the translational level is significantly altered by carbon source, providing an insight into the complex protein interactions across carbon sources and offering theoretical clues for further study to elucidate their general regulatory mechanism to adapt to different nutrient environments.

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Discovery of numerous clusters of spontaneous mutations in the specific-locus test in mice necessitates major increases in estimates of doubling doses

Selby

1998

Mutation and Evolution

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Environmental chemical mutagens and genetic risks: Lessons from radiation genetics

Cited by 6 publications

References 16 publications

Meiotic and mitotic instability of two EMS-produced centric fragments in the haplodiploid wasp Nasonia vitripennis

Meiotic and mitotic instability of two EMS-produced centric fragments in the haplodiploid wasp Nasonia vitripennis

iTRAQ-Based Comparative Proteomic Analysis of Acinetobacter baylyi ADP1 Under DNA Damage in Relation to Different Carbon Sources

Discovery of numerous clusters of spontaneous mutations in the specific-locus test in mice necessitates major increases in estimates of doubling doses

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