Environmental chemical hazards and child health

Mello-da-Silva, Carlos Augusto; Fruchtengarten, Ligia Veras Gimenez

doi:10.2223/jped.1409

Cited by 15 publications

(14 citation statements)

References 22 publications

(20 reference statements)

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“…developmental and metabolic disorders, reproductive disturbances, and malignant tumors (9)(10)(11)(12)(13)(14). Children are especially susceptible to adverse effects of EED exposure due to their physiological characteristics such as small body size and limited capacity to metabolize EED (9,10).…”

Section: Environmental Endocrine Disruptors Promote Invasion and Metamentioning

confidence: 99%

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Environmental endocrine disruptors promote invasion and metastasis of SK-N-SH human neuroblastoma cells

Xiao¹

2009

Oncol Rep

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Abstract. Neuroblastoma (NB) is the most common pediatric extracranial cancer. Metastasis is the main cause of mortality in NB patients. Currently, little is known about the risk factors and their mechanisms that cause metastasis. Environmental endocrine disruptors (EED) are recently identified risk factors associated with various human diseases including malignant tumors. Our previous studies have implicated the role of di(2-ethylhexyl) phthalate (DEHP) and bisphenol A (BPA), two of the most common EED, in neuroblastoma cell proliferation. Here, we further investigated the effects of DEHP, BPA as well as 17ß-estradiol (E 2 ) on the invasion and metastasis of human neuroblastoma SK-N-SH cells in vitro. SK-N-SH cells expressed estrogen receptor (ER)-ß, matrix metalloproteinases-2 (MMP-2), MMP-9 and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) at readily detectable levels. 50 μM DEHP, 0.1 μM BPA and 10 μM E 2 exposure all resulted in enhanced motility and invasiveness of SK-N-SH cells (P<0.001), elevated expression of MMP-2 and MMP-9, and decreased expression of TIMP-2 (P<0.01). Furthermore, phosphorylation of Akt (Ser473) was also induced following the exposure (P<0.01). Importantly, both ER antagonist ICI182,780 and phosphoinositide 3-kinase (PI3K) specific inhibitor LY294002 significantly inhibited the DEHP, BPA, or E 2 -induced cell migration and invasion, as well as the disregulation of MMP-2, MMP-9 and TIMP-2 expression. ICI182,780 may have worked through abolishing Akt (Ser473) phosphorylation. In conclusion, DEHP, BPA, and E 2 potently promote invasion and metastasis of neuroblastoma cells through overexpression of MMP-2 and MMP-9 as well as downregulation of TIMP-2. ER-dependent pathway and PI3K/Akt pathway are involved, which may become potential therapeutic targets for neuroblastoma treatment.

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Section: Environmental Endocrine Disruptors Promote Invasion and Metamentioning

confidence: 99%

“…Children are especially susceptible to adverse effects of EED exposure due to their physiological characteristics such as small body size and limited capacity to metabolize EED (9,10). Di(2-ethylhexyl) phthalate (DEHP) and bisphenol A (BPA) are most common compounds in EED.…”

Section: Environmental Endocrine Disruptors Promote Invasion and Metamentioning

confidence: 99%

Environmental endocrine disruptors promote invasion and metastasis of SK-N-SH human neuroblastoma cells

Xiao¹

2009

Oncol Rep

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“…Furthermore, many studies have shown that BPA influences the promotion or progression of many cancers such as seminoma, prostate cancer, and breast cancer (Bouskine et al, 2009;Hess-Wilson, 2009;LaPensee et al, 2010). In particular, children are vulnerable to EE exposure because they have a relatively large body surface area and limited catabolism (Mello-da-Silva and Fruchtengarten, 2005). Therefore, investigation of the relationship between BPA exposure and pediatric tumors is very important.…”

Section: Introductionmentioning

confidence: 99%

Effects of bisphenol A on decreasing the percentage and promoting the growth of stem cell-like cells from SK-N-SH human neuroblastoma cells

Zheng¹,

Qi²,

Yang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Bisphenol A (BPA) is an industrial contaminant and is reported to be a risk factor associated with the development of tumors. In our previous studies, we have shown that BPA promoted the growth of SK-N-SH human neuroblastoma cells and increased their invasion and metastasis. In this study, we further investigated the effects of BPA and 17b-estradiol (E 2 ) on the stem cell-like cells from SK-N-SH cells. Detection of stem cell markers, proliferation assay, and clonogenic analysis showed that the side-population (SP) of SK-N-SH cells had properties similar to those of stem cells. BPA or E 2 exposure decreased the percentage of SP cells and the expression of stem cell-marker proteins. BPA and E 2 promoted the growth of non-SP cells to a greater extent than of SP cells; in addition, they significantly increased the 2987 BPA promotes the growth of cancer stem cell-like SP cells ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 2986-2993 (2015 growth of SP cells. Thus, BPA has effects on stem cell-like cells, which induce tumor formation, and thus, BPA is an environmental factor that plays an important role in the development of neuroblastoma.

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“…Environmental factors have been implicated as causative factors for many human diseases such as deformity, reproductive disturbances, and breast cancer. Children are especially vulnerable to EED exposure because of their physiological characteristics [2]. Based on consistent epidemiological studies, the occurrence of childhood asthma, neurologic developmental disorders, hypospadias, and early puberty has increased in the last 10 years [2].…”

mentioning

confidence: 99%

“…Children are especially vulnerable to EED exposure because of their physiological characteristics [2]. Based on consistent epidemiological studies, the occurrence of childhood asthma, neurologic developmental disorders, hypospadias, and early puberty has increased in the last 10 years [2]. The increasing incidence of childhood cancer such as acute lymphoblastic leukemia, brain cancer, and testicular cancer is believed to be related to the presence of chemical agents in the environment [3,4].…”

mentioning

confidence: 99%