Environmental and host factors shaping the gut microbiota diversity of brown frog Rana dybowskii

Tong, Qing; Cui, Li-Yong; Hu, Zongfu; Du, Xiaodong; Abid, Hayat Muhammad; Wang, Hongbin

doi:10.1016/j.scitotenv.2020.140142

Cited by 27 publications

(30 citation statements)

References 54 publications

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“…Firmicutes [2,19,24]. However, in this study, no bacterial phylum was found to signi cantly differ in abundance among the control, gentamicin, and recovery groups.…”

Section: Variation In the Gut Microbiota Compositioncontrasting

confidence: 63%

“…The ability of the gut microbiota to recover to the baseline level after stopping antibiotics may vary depending on differences in the antibiotic administration, the host species, the community context, and environmental reservoirs [2,22]. In the present study, the gentamicin bath signi cantly changed the composition of the gut bacterial community; however, after 7 days, the gut microbiota was still similar to that during the gentamicin bath.…”

Section: Variation In Gut Microbiota Diversitycontrasting

confidence: 47%

“…In the present study, the gentamicin bath signi cantly changed the composition of the gut bacterial community; however, after 7 days, the gut microbiota was still similar to that during the gentamicin bath. After mice were treated with antibiotics, it was found that the structure of the microbiome in the intestines of the mice was signi cantly changed and that the structure of the microbiome could be quickly restored to its previous con guration after the treatment [2]. However, previous studies examined antibiotic treatment in aquatic animals, and within a few days of antibiotic consumption, the intestinal microbiota biodiversity was reduced, while the initial bacterial community composition rarely fully recovered [23].…”

Section: Variation In Gut Microbiota Diversitymentioning

confidence: 99%

“…The effect of antibiotics on the gut bacterial community persists after the withdrawal of antibiotic treatment. Severe antibiotic pressure resulted in non-reversible, long-lasting alterations in the gut microbiota [2].…”

Section: Variation In Gut Microbiota Diversitymentioning

confidence: 99%

“…The diversity of bacterial communities is closely related to animal health, especially the gut diversity of the animal microbiota [1]. The gut bacterial community is dynamic, and its composition is continually changing to cope with changes in the internal and external environment [2]. The gut bacterial community can form a barrier against pathogens, producing many essential products and playing a role in the digestion, gut morphology, immunity, and regulation of host gene expression [3].…”

Section: Introductionmentioning

confidence: 99%

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Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

Tong

Cui

Jia

et al. 2021

Preprint

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Background: Amphibians frequently receive an antibiotic bath after feedlot placement to control bacterial diseases. The potential collateral effect of these antibiotics on the frog microbiota is largely unknown. Antibiotics are frequently employed to examine the role of the gut microbiota. Existing research relies mainly on oral antibiotics, but knowledge regarding the effects of antibiotics on the gut microbiota through a bath or local antimicrobial therapies is limited. Results: The gut microbiota of gentamicin, recovered, and control Rana dybowskii groups were compared by Illumina high-throughput sequencing, and the functional profiles were analysed using a phylogenetic investigation of communities by the reconstruction of unobserved states (PICRUSt). Furthermore, the relationship between gut microbiota structures and forecast function compositions was determined. The results showed that the alpha diversity indices were significantly reduced by the gentamicin bath, which significantly changed the composition of the gut microbiota. After 7 days, the gut microbiota was still similar to that during the gentamicin bath. Forty-four indicator species were selected at the genus level, namely, 42 species indicating the control group and 2 species indicating the gentamicin and recovery groups. Potential pathogenic bacteria belonging to Aeromonas, Citrobacter, and Chryseobacterium significantly decreased after the gentamicin bath. The community similarity assays did not show an obvious discrepancy in the functional composition between the gentamicin and control frogs, indicating that the functions of the gut bacterial community were highly redundant. Conclusions: The gentamicin bath significantly reduced the alpha diversity of the gut microbiota of R. dybowskii. Gentamicin significantly changed the structure of the gut microbiota, and the gut microbiotas exhibited weak resilience and did not totally recover after seven days. The gentamicin bath did not change the functional composition of the gut microbiota of R. dybowskii, and there was no significant correlation between the composition of the gut microbiota and the functional composition, illustrating the high intestinal functional redundancy of the frog gut bacterial community. This work offers basic data for upcoming research, including the establishment of the amphibian gut microbiota and local antibiotic administration, and has important implications for aquaculture management and amphibian conservation.

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“…Firmicutes [2,19,24]. However, in this study, no bacterial phylum was found to signi cantly differ in abundance among the control, gentamicin, and recovery groups.…”

Section: Variation In the Gut Microbiota Compositioncontrasting

confidence: 63%

Section: Variation In Gut Microbiota Diversitycontrasting

confidence: 47%

Section: Variation In Gut Microbiota Diversitymentioning

confidence: 99%

Section: Variation In Gut Microbiota Diversitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

Tong

Cui

Jia

et al. 2021

Preprint

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The succession of gut microbiota in the concave‐eared torrent frog (Odorrana tormota) throughout developmental history

Shi

Deng

et al. 2023

Ecology and Evolution

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Comparative analysis of gut microbial composition and potential functions in captive forest and alpine musk deer

Jiang

Song

Wang

et al. 2022

Appl Microbiol Biotechnol

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Gut microbiota forms a unique microecosystem and performs various irreplaceable metabolic functions for ruminants. The gut microbiota is important for host health and provides new insight into endangered species conservation. Forest musk deer (FMD) and alpine musk deer (AMD) are typical small ruminants, globally endangered due to excessive hunting and habitat loss. Although nearly 60 years of captive musk deer breeding has reduced the hunting pressure in the wild, fatal gastrointestinal diseases restrict the growth of captive populations. In this study, 16S rRNA high-throughput sequencing revealed the differences in gut microbiota between FMD and AMD based on 166 fecal samples. The alpha diversity was higher in FMD than in AMD, probably helping FMD adapt to different and wider habitats. The ß-diversity was higher between adult FMD and AMD than juveniles and in winter than late spring. The phylum Firmicutes and the genera Christensenellaceae R7 group, Ruminococcus, Prevotellaceae UCG-004, and Monoglobus were significantly higher in abundance in FMD than in AMD. However, the phylum Bacteroidetes and genera Bacteroides, UCG-005, Rikenellaceae RC9 gut group, and Alistipes were significantly higher in AMD than FMD. The expression of metabolic functions was higher in AMD than in FMD, a beneficial pattern for AMD to maintain higher energy and substance metabolism. Captive AMD may be at higher risk of intestinal diseases than FMD, with higher relative abundances of most opportunistic pathogens and the expression of disease-related functions. These results provide valuable data for breeding healthy captive musk deer and assessing their adaptability in the wild. Key points • Alpha diversity of gut microbiota was higher in FMD than that in AMD • Expression of metabolic and disease-related functions was higher in AMD than in FMD

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Environmental and host factors shaping the gut microbiota diversity of brown frog Rana dybowskii

Cited by 27 publications

References 54 publications

Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

The succession of gut microbiota in the concave‐eared torrent frog (Odorrana tormota) throughout developmental history

Comparative analysis of gut microbial composition and potential functions in captive forest and alpine musk deer

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