Enumeration of
            <i>Salmonella</i>
            Bacteria in Food and Feed Samples by Real-Time PCR for Quantitative Microbial Risk Assessment

Malorny, Burkhard; Löfström, Charlotta; Wagner, Martin; Krämer, Nadine; Hoorfar, Jeffrey

doi:10.1128/aem.02489-07

Cited by 145 publications

(101 citation statements)

References 31 publications

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“…It is frequently isolated from the marine environment where it can remain viable for several hours (Malorny et al, 2008;Westrell et al, 2009). Contamination with Salmonella has been reported in surface water used for recreational purposes, source of drinking water (Till et al, 2008) and irrigation (Gannon et al 2004) underlining the possible risk associated to the use of such contaminated water.…”

Section: Introductionmentioning

confidence: 99%

Detection of Bacterial Pathogens in River Water Using Multiplex-PCR

Kinge¹,

Mbewe²,

Sithebe³

2012

Polymerase Chain Reaction

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Section: Introductionmentioning

confidence: 99%

Detection of Bacterial Pathogens in River Water Using Multiplex-PCR

Kinge¹,

Mbewe²,

Sithebe³

2012

Polymerase Chain Reaction

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“…LightCycler PCR (LCPCR) is a specific real-time PCR system enabling rapid and reliable detection of the specific PCR product with probe-based technology and high sensitivity (3). Still, complementation of PCR by standard culture is required for the elimination of possible false negative and/or variable PCR results related to inhibitory substances within the process (29), and for avoiding false positive results due to amplification of target DNA from dead/non-culturable/injured Salmonella cells in the sample (20).…”

Section: Introductionmentioning

confidence: 99%

Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system

Temelli¹,

Kahya²,

Ata³

et al. 2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:This study aims to determine the presence of Salmonella in naturally contaminated grade A eggs by the standard culture method International Organization for Standardization Method 6579 (ISO) and a specific real-time PCR system (LightCycler PCR-LCPCR) to complement ISO. A total of 1635 eggs pooled into 101 samples were randomly collected within one year period from 20 different retail markets in Bursa, Turkey, carrying eggs of 16 large egg producers/suppliers of 5 cities with intensive layer production. Preparation of the egg and shell for analyses, Salmonella isolations and identifications, and detections were performed according to ISO 6887-4:2003, ISO 6579 and LCPCR, respectively. Overall Salmonella detection rate by ISO and LCPCR were 15.8 % (16/101) and 46.5 % (47/101), respectively. Out of 101 inner parts, Salmonella was detected in 11 (10.9 %) samples by ISO, and in 31 (30.7 %) samples by LCPCR. Six of 101 shell samples (5.9 %) were found to harbor Salmonella by ISO, while 18 (17.8 %) shells were positive by LCPCR. All isolates were determined as Salmonella enterica subsp. enterica serovar Enteritidis. These findings indicate considerably high Salmonella contamination in retail grade A eggs. This should be under routine monitoring by rapid methods such as PCR, complemented by standard culture to evaluate and assess the significance of risk for public health.

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“…Weaknesses: QMRA is still hampered by the lack of quantitative data, and often assumptions that generate high degrees of uncertainty have to be included (Malorny et al, 2008). Most of the QMRA studies are judged as very difficult to (a) pinpoint quickly and efficiently the assumptions and uncertainties involved in the methods employed, (b) identify quickly data and sources used, and (c) comprehend the innovative points in each study.…”

mentioning

confidence: 99%

Critical review of methodology and application of risk ranking for prioritisation of food and feed related issues, on the basis of the size of anticipated health impact

Fels‐Klerx

Asselt

Raley³

et al. 2015

EFSA Supporting Publications

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Document VersionPublisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): van der Fels-Klerx, H. J., van Asselt, E. D., Raley, M., Poulsen, M., Korsgaard, H. B., Bredsdorff, L., ... Frewer, L. (2015). Critical review of methodology and application of risk ranking for prioritisation of food and feed related issues, on the basis of the size of anticipated health impact. Europen Food Safety Authority. (EFSA Supporting Publications; No. 710E, Vol. 12(1) ABSTRACTThis study aimed to critically review methodologies for ranking of risks related to feed/food safety and nutritional hazards, on the basis of their anticipated human health impact. An extensive systematic literature review was performed to identify and characterize the available methodologies for risk ranking in the fields of feed and food safety and nutritional hazards, as well as the socio-economic field. Risk ranking methods from the environmental field were studied as well to determine whether approaches used in this field could also be applied for ranking human health risks related to feed and food safety and nutritional hazards. The review used a predefined search protocol. It covered the bibliographic databases Scopus, CAB Abstracts, Web of Sciences, and PubMed over the period 1993-2013. All references obtained were stored into an Endnote database and evaluated for their relevance. All references deemed to be relevant were studied in-depth so as to characterize the risk ranking method described. Characteristics of each method were stored in an Excel database. The methods for risk ranking were then grouped into method categories, which were described in general. These groups included: risk assessment, comparative risk assessment, risk ratio method, scoring method, cost of illness, DALY/QALY, willingness to pay, multi criteria decision analysis, risk matrix, flow charts/decision trees and expert judgment methods. Based on the characteristics of the individual methods and the method categories, an overarching framework was developed for selection of the appropriate method(s) that could be used for risk ranking of feed and food related hazards, on the basis of human health impact. This framework has the format of a decision tool, with which -given the characteristics of the risk ranking question at hand -the most appropriate method(s) can be selected. Application of this overall framework to several case studies showed it can be a useful tool for risk managers/assessors to select the most suitable method for risk ranking of feed/food and diet related hazards, on the basis of expected human health impact. © RIKILT, 2015 DISCLAIMERThe present document has been produced and adopted by the bodies identified above as author(s). This task has been carried out exclusively by the author(s) in the context of a contract between the European Food Safety Authority and the author(s), awarded following a tender procedure. The present document is published complying with the transparency principle to which the Authority is subject. It...

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Enumeration of Salmonella Bacteria in Food and Feed Samples by Real-Time PCR for Quantitative Microbial Risk Assessment

Cited by 145 publications

References 31 publications

Detection of Bacterial Pathogens in River Water Using Multiplex-PCR

Detection of Bacterial Pathogens in River Water Using Multiplex-PCR

Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system

Critical review of methodology and application of risk ranking for prioritisation of food and feed related issues, on the basis of the size of anticipated health impact

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