Enumeration, antimicrobial resistance and genomic characterization of extended-spectrum β-lactamases producing Escherichia coli from supermarket chicken meat in the United Arab Emirates

Habib, Ihab; Al‐Rifai, Rami H.; Mohamed, Mohamed-Yousif Ibrahim; Ghazawi, Akela; Abdalla, Afra; Khalifa, Hazim O.; Khan, Mushtaq Ahmad

doi:10.1016/j.ijfoodmicro.2023.110224

Cited by 7 publications

(4 citation statements)

References 42 publications

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“…In the current study, mcr-1-positive IncI2 and IncX4 plasmids were isolated. These two plasmid types were closely associated with mcr-1-related AMR associated with non-camel livestock in the UAE, as documented in several studies over the past 4 years [27][28][29]. IncI2 plasmids have been identified in several mcr-1 cases on various hosts worldwide [32], while the IncX4 plasmid type has been recognized as the primary carrier of the mcr-1 gene in isolates from healthy individuals in China [33], as well as in Enterobacteriaceae isolates carrying carbapenem and mcr-1 resistance genes simultaneously from clinical patients in Thailand [34].…”

Section: Discussionsupporting

confidence: 56%

“…It is noteworthy that mcr-1 -positive Enterobacterales isolates have been previously reported in the UAE; however, these were specifically associated with poultry [ 27 , 28 , 29 ], whereas the current study focused on colistin-resistant E. coli from camels, indicating a relatively high frequency of mcr-1 -positive E. coli (22%) within a farm camel population in the UAE. In fact, more than seventy-five genotypic sequence types (STs) of E. coli have been reported to carry mcr-1 [ 25 ].…”

Section: Discussionmentioning

confidence: 59%

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First Report of Colistin-Resistant Escherichia coli Carrying mcr-1 IncI2(delta) and IncX4 Plasmids from Camels (Camelus dromedarius) in the Gulf Region

Ghazawi,

Strepis,

Anes

et al. 2024

Antibiotics

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Addressing the emergence of antimicrobial resistance (AMR) poses a significant challenge in veterinary and public health. In this study, we focused on determining the presence, phenotypic background, and genetic epidemiology of plasmid-mediated colistin resistance (mcr) in Escherichia coli bacteria isolated from camels farmed in the United Arab Emirates (UAE). Fecal samples were collected from 50 camels at a Dubai-based farm in the UAE and colistin-resistant Gram-negative bacilli were isolated using selective culture. Subsequently, a multiplex PCR targeting a range of mcr-genes, plasmid profiling, and whole-genome sequencing (WGS) were conducted. Eleven of fifty camel fecal samples (22%) yielded colonies positive for E. coli isolates carrying the mcr-1 gene on mobile genetic elements. No other mcr-gene variants and no chromosomally located colistin resistance genes were detected. Following plasmid profiling and WGS, nine E. coli isolates from eight camels were selected for in-depth analysis. E. coli sequence types (STs) identified included ST7, ST21, ST24, ST399, ST649, ST999, and STdaa2. Seven IncI2(delta) and two IncX4 plasmids were found to be associated with mcr-1 carriage in these isolates. These findings represent the first identification of mcr-1-carrying plasmids associated with camels in the Gulf region. The presence of mcr-1 in camels from this region was previously unreported and serves as a novel finding in the field of AMR surveillance.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 59%

First Report of Colistin-Resistant Escherichia coli Carrying mcr-1 IncI2(delta) and IncX4 Plasmids from Camels (Camelus dromedarius) in the Gulf Region

Ghazawi,

Strepis,

Anes

et al. 2024

Antibiotics

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“…Antimicrobial resistance is a growing global concern in public health [ 96 , 97 , 98 , 99 , 100 , 101 , 102 ]. The rise of antibiotic-resistant bacterial infections has resulted in a significant number of deaths worldwide.…”

Section: Public Health Impactsmentioning

confidence: 99%

“…In both human and veterinary medicine, this issue is particularly pronounced in underdeveloped nations because of the unrestricted availability of antibiotics and inadequate infection control measures [ 96 , 97 , 98 , 99 ]. Shockingly, according to a global study, antimicrobial resistance claimed more lives in 2019 than either HIV/AIDS or malaria [ 100 ]. One of the key difficulties is the transmission of antibiotic-resistant microorganisms across various sectors, including humans, animals, and food chains.…”

Section: Public Health Impactsmentioning

confidence: 99%

Veterinary Drug Residues in the Food Chain as an Emerging Public Health Threat: Sources, Analytical Methods, Health Impacts, and Preventive Measures

Khalifa,

Shikoray,

Mohamed

et al. 2024

Foods

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Veterinary medications are necessary for both contemporary animal husbandry and food production, but their residues can linger in foods obtained from animals and pose a dangerous human risk. In this review, we aim to highlight the sources, occurrence, human exposure pathways, and human health effects of drug residues in food-animal products. Following the usage of veterinary medications, pharmacologically active compounds known as drug residues can be found in food, the environment, or animals. They can cause major health concerns to people, including antibiotic resistance development, the development of cancer, teratogenic effects, hypersensitivity, and disruption of normal intestinal flora. Drug residues in animal products can originate from variety of sources, including water or food contamination, extra-label drug use, and ignoring drug withdrawal periods. This review also examines how humans can be exposed to drug residues through drinking water, food, air, and dust, and discusses various analytical techniques for identifying these residues in food. Furthermore, we suggest some potential solutions to prevent or reduce drug residues in animal products and human exposure pathways, such as implementing withdrawal periods, monitoring programs, education campaigns, and new technologies that are crucial for safeguarding public health. This review underscores the urgency of addressing veterinary drug residues as a significant and emerging public health threat, calling for collaborative efforts from researchers, policymakers, and industry stakeholders to develop sustainable solutions that ensure the safety of the global food supply chain.

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Genomic profiling of extended-spectrum β-lactamase-producing Escherichia coli from Pets in the United Arab Emirates: Unveiling colistin resistance mediated by mcr-1.1 and its probable transmission from chicken meat – A One Health perspective

Habib,

Elbediwi,

Mohteshamuddin

et al. 2023

Journal of Infection and Public Health

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Enumeration, antimicrobial resistance and genomic characterization of extended-spectrum β-lactamases producing Escherichia coli from supermarket chicken meat in the United Arab Emirates

Cited by 7 publications

References 42 publications

First Report of Colistin-Resistant Escherichia coli Carrying mcr-1 IncI2(delta) and IncX4 Plasmids from Camels (Camelus dromedarius) in the Gulf Region

First Report of Colistin-Resistant Escherichia coli Carrying mcr-1 IncI2(delta) and IncX4 Plasmids from Camels (Camelus dromedarius) in the Gulf Region

Veterinary Drug Residues in the Food Chain as an Emerging Public Health Threat: Sources, Analytical Methods, Health Impacts, and Preventive Measures

Genomic profiling of extended-spectrum β-lactamase-producing Escherichia coli from Pets in the United Arab Emirates: Unveiling colistin resistance mediated by mcr-1.1 and its probable transmission from chicken meat – A One Health perspective

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