Enucleated GV Oocytes as Recipients of Embryonic Nuclei in the G1, S, or G2 Stages of the Cell Cycle

Mohammed, Abd El-Nasser Ahmed; Karasiewicz, Jolanta; Kubacka, Joanna; Gręda, Paweł; Modliński, Jacek A.

doi:10.1089/cell.2009.0107

Cited by 10 publications

(23 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Xenopus, Gurdon et al ruptured the GV by a needle to promote the mixing of GV contents with the oocyte cytoplast prior to nuclear transfer and significantly increased the efficiency of nuclear reprogramming [29]. The transfer of mouse somatic cells into GV oocytes confirmed that GV material was essential for nuclear reprogramming [30]. Nuclear components extracted from the GVs have been demonstrated to be important in facilitating reprogramming of the introduced foreign nuclei in mammals [31].…”

Section: Nuclear Reprogramming Begins From Oogenesismentioning

confidence: 99%

“…The results imply that GV stage cytoplasts are incompetent to support cloned embryo development. An earlier study by Mohammed et al also concluded that GV stage oocytes were unsuitable as recipients for nuclear transfer [30]. It is thought that the reprogramming factor(s) responsible for embryonic stem cells reside in the nucleus [35,36].…”

Section: Nuclear Reprogramming Begins From Oogenesismentioning

confidence: 99%

“…The MII stage oocyte creates a better environment for SCNT than either the GV or metaphase I stage oocytes [30,42,43].…”

Section: Second Metaphase Stage Cytoplasts As Recipientsmentioning

confidence: 99%

See 2 more Smart Citations

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Yin¹,

Liu²,

Bou³

et al. 2014

Front. Agr. Sci. Eng.

View full text Add to dashboard Cite

Oocytes are unique cells with the inherent capability to reprogram nuclei. The reprogramming of the somatic nucleus from its original cellular state to a totipotent state is essential for term development after somatic cell nuclear transfer. The nuclear-associated factors contained within oocytes are critical for normal fertilization by sperm or for somatic cell nuclear reprogramming. The chromatin of somatic nuclei can be reprogrammed by factors in the egg cytoplasm whose natural function is to reprogram sperm chromatin. The oocyte first obtains its reprogramming capability in the early fetal follicle, and then its capacity is enriched in the late growth phase and reaches its highest capability for reprogramming as fully-grown germinal vesicle oocytes. The cytoplasmic milieu most likely contains all of the specific transcription and/or reprogramming factors necessary for cellular reprogramming. Certain transcription factors in the cytoplast may be critical as has been demonstrated for induced pluripotent stem cells. The maternal pronucleus exerts a predominant, transcriptiondependent effect on embryo cytofragmentation, with a lesser effect imposed by the ooplasm and the paternal pronucleus. With deep analysis of transcriptomics in oocytes and early developmental stage embryos more maternal transcription factors inducing cellular reprogramming will be identified.

show abstract

Section: Nuclear Reprogramming Begins From Oogenesismentioning

confidence: 99%

Section: Nuclear Reprogramming Begins From Oogenesismentioning

confidence: 99%

See 1 more Smart Citation

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Yin¹,

Liu²,

Bou³

et al. 2014

Front. Agr. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Treatments of dysfunctions or improvements of physiological and reproductive performances were achieved through feed supplements Mohammed and Al-Hozab, 2020;Sheoran et al, 2018) and assisted reproductive techniques (Mohammed et al, 2010;Mohammed et al, 2012;Mohammed 2019). The continuous interests for the use of dietary supplements have grown to human and animals as well in order to improve their productive, reproductive and therapeutic performances (Gonzalez-Uarquin et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effects of Myo-inositol on Physiological and Reproductive Traits through Blood Parameters, Oocyte Quality and Embryo Transfer in Mice

Mohammed¹,

Alshaheen²,

Al-Suwaiegh³

2020

IJAR

View full text Add to dashboard Cite

Background: The beneficial effects of myo-inositol to mammals on physiological and reproductive functions and treatments of malfunctions have been reported. The aims of present study were to assess the efficacy of myo-inositol supplementation on physiological and reproductive performances through blood parameters, oocyte quality and embryo transfer in mice. Methods: Seventy-five virgin female mice were classified into three equal groups; control group (T1) versus two myo-inositol groups (T2 receive 30 mmol/l and T3 receive 60 mmol/l). Changes of body temperature, blood oxygen and glucose, heart rate, blood parameters (RBCs, WBCs, hematocrit, glucose and total protein), oocyte quality (cumulus enclosed, diameter and brilliant cresyl blue stain) and reproductive performances (litter weight and size) were determined. Result: The results indicated that myo-inositol supplementation resulted in significant increase in values of RBCs, WBCs, hematocrit and total protein in addition to significant hypoglycemia. The quality of oocytes in case of cumulus enclosed, diameter and brilliant cresyl blue staining and reproductive performances were improved due to myo-inositol supplementation. Furthermore, myo-inositol supplementation effectively alleviated hypothermia and hyperglycemia following general anaesthesia.

show abstract

“…Assisted reproductive technology (ART) is the application of laboratory or field technology to gametes and/or embryos for the purposes of reproduction (Mohammed et al, 2005;Mohammed, 2006a;Mohammed, 2006b;Mohammed, 2008;Mohammed et al, 2008;Mohammed, 2009a, and b;Mohammed, 2010;Mohammed et al, 2010;. Reproductive technology on mammals has made significant strides over the past fifty years.…”

Section: Introductionmentioning

confidence: 99%

Assisted Reproductive Technology in Mammals

2011

Egyptian Journal of Animal Production

View full text Add to dashboard Cite

This review describes the use of modern reproductive biotechnologies or assisted reproductive techniques (ART) including artificial insemination, embryo transfer, in vitro fertilization, gamete/embryo micromanipulation, semen sexing, genome resource banking, and somatic cell nuclear transfer (cloning) for mammalian species. Such biotechnologies allow more offspring to be obtained from selected parents to increase productivity of milk and meat, reduce the interval between generations, therapy of diseases and conservation of endangered mammalian species. Practically, current reproductive biotechnologies are species-specific because of differences in estrous cycle, seasonality, structural anatomy, gamete physiology and site for semen deposition or method of embryo transfer.

show abstract

Enucleated GV Oocytes as Recipients of Embryonic Nuclei in the G1, S, or G2 Stages of the Cell Cycle

Cited by 10 publications

References 35 publications

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Effects of Myo-inositol on Physiological and Reproductive Traits through Blood Parameters, Oocyte Quality and Embryo Transfer in Mice

Assisted Reproductive Technology in Mammals

Contact Info

Product

Resources

About