Effects of Myo-inositol on Physiological and Reproductive Traits through Blood Parameters, Oocyte Quality and Embryo Transfer in Mice

Abstract

Background: The beneficial effects of myo-inositol to mammals on physiological and reproductive functions and treatments of malfunctions have been reported. The aims of present study were to assess the efficacy of myo-inositol supplementation on physiological and reproductive performances through blood parameters, oocyte quality and embryo transfer in mice. Methods: Seventy-five virgin female mice were classified into three equal groups; control group (T1) versus two myo-inositol groups (T2 receive 30 mmol/l and T3 receive 60 mmol/l). Changes of body temperature, blood oxygen and glucose, heart rate, blood parameters (RBCs, WBCs, hematocrit, glucose and total protein), oocyte quality (cumulus enclosed, diameter and brilliant cresyl blue stain) and reproductive performances (litter weight and size) were determined. Result: The results indicated that myo-inositol supplementation resulted in significant increase in values of RBCs, WBCs, hematocrit and total protein in addition to significant hypoglycemia. The quality of oocytes in case of cumulus enclosed, diameter and brilliant cresyl blue staining and reproductive performances were improved due to myo-inositol supplementation. Furthermore, myo-inositol supplementation effectively alleviated hypothermia and hyperglycemia following general anaesthesia.

The Potential Impacts of Selecting Viable Oocytes on Further Embryonic Development in Mammals: A Review

“…A plethora of molecules modified by the nutritional intake as amino acids, pyruvate, fatty acids, inorganic ions, second messengers, cytokines, growth factors effect on ovarian follicle development, oocyte quality, maturation and further embryonic development and live birth in different mammalian species (Mohammed 2018, Mohammed et al, 2020, 2024aHye et al, 2020;Thuy Van et al, 2020;Fabozzi et al, 2021;Costa et al, 2023;Garza et al, 2023;Silva et al, 2023;Liu et al, 2024).…”

“…The correlation between follicular fluid characteristics and oocyte quality was complex and interesting field of research, with promising potential for improving assisted reproductive technologies (Mohammed et al, 2005(Mohammed et al, , 2020. Follicular fluid (FF) surrounds the oocyte within the ovarian follicle and provides a crucial microenvironment for growth and development of the oocyte.…”

“…Other components still need further studies to define the changes at cellular level (Mohammed et al, 2019b). Finally, the research on the correlation between follicular fluid characteristics and oocyte quality was promising but still evolving due to differences in experimental design, animal species and the analytical methods used (Mohammed et al, 2020).…”

“…Significant strides of progresses is achieved in selecting the higher developmental competence oocytes through the characters of ovarian follicles, oocytes morphology, follicular fluid and gene expression (Mohammed et al, 2005;Mlyczyńska et al, 2023). It has been known that several factors affect the ovarian follicle diameters and the contents of oocytes and follicular fluid parameters (Contreras-Solís et al, 2021;Castro-Modesto et al, 2022;Mohammed et al, 2020Mohammed et al, , 2024aCañón-Beltrán et al, 2024).…”