Entry-Prohibited Effect of kHz Pulsed Magnetic Field Upon Interaction Between SPIO Nanoparticles and Mesenchymal Stem Cells

Wang, Peng; Gu, Ning; Sun, Jianfei; Ma, Siyu; Ning, Guangfu; Chen, Wu; Wang, Bin; Ye, Dewen; Chen, Bin; Yang, Yuzhi; Jiang, Qing

doi:10.1109/tbme.2019.2931774

Cited by 15 publications

(9 citation statements)

References 35 publications

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“…According to a previous study, the iron content of rat adipose-derived stem cells was 0.31 ± 0.02 pg/cell. 36 This is consistent with our results, wherein the iron content of untreated hGFs was very low ( Figure S3 ).…”

Section: Discussionsupporting

confidence: 93%

Superparamagnetic Iron Oxide Nanoparticles Protect Human Gingival Fibroblasts from Porphyromonas gingivalis Invasion and Inflammatory Stimulation

Chen

Zhang

Qin

et al. 2022

IJN

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Introduction:Modulating the inflammatory response of human gingival fibroblasts (hGFs) is important for the control of periodontal inflammation because it is a key event in the pathogenesis of periodontitis. Here, we aimed to determine whether polyglucose sorbitol carboxymethyl ether (PSC)-coated superparamagnetic iron oxide nanoparticles (SPIONs) protect hGFs against invasion and inflammatory stimulation by Porphyromonas gingivalis (P. gingivalis). Methods: First, we determined the cytotoxicity and antimicrobial activity of PSC-SPIONs. Then, their effects on invasion of hGFs by P. gingivalis were evaluated by counting invading P. gingivalis, fluorescence staining, and transmission electron microscopy. The effect of PSC-SPIONs on inflammation in hGFs induced by P. gingivalis lipopolysaccharide was evaluated by measurement of reactive oxygen species (ROS), and enzyme-linked immunosorbent assays, quantitative reverse transcription-polymerase chain reaction, and Western blotting of key indicator molecules. The effects of dimercaptosuccinic acid (DMSA)-coated SPIONs and the free form of PSC alone were also tested and compared with those of PSC-SPIONs. Results: PSC-SPIONs (25 μg/mL) are cytocompatible with hGFs and exhibit no antimicrobial effects on P. gingivalis. However, they inhibit invasion of hGFs by P. gingivalis at 15 μg/mL. They also decrease ROS production and inflammatory cytokine secretion by hGFs at 5, 15, and 25 μg/mL, by downregulating activation of the nuclear factor-kappa B signaling pathway. Furthermore, PSC alone does not inhibit inflammation, while DMSA-SPIONs do. This indicates that the nanosize effects of PSC-SPIONs, rather than their coating material, play the dominant role in their anti-inflammatory activity. Conclusion: PSC-SPIONs protect hGFs against P. gingivalis invasion and inflammatory stimulation. Thus, they have potential for clinical application in control of periodontal inflammation.

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Section: Discussionsupporting

confidence: 93%

Superparamagnetic Iron Oxide Nanoparticles Protect Human Gingival Fibroblasts from Porphyromonas gingivalis Invasion and Inflammatory Stimulation

Chen

Zhang

Qin

et al. 2022

IJN

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“…Similar results have been reported in studies evaluating the potential cytotoxicity of SPIO nanoparticles in different types of cells. 31 , 35 , 36 Work by Liu et al suggested that there was no significant cytotoxicity of SPIO nanoparticles in dendritic cells when the cells were treated with 50 μg/mL SPIO nanoparticles, 35 while our results showed that the SPIO nanoparticles had no cytotoxicity on SGNs at concentrations of 300 μg/mL, which showed better biocompatibility. In fact, the cytotoxicity of SPIO nanoparticles varies with particle size and cell type and is related to the type and stability of the nanoparticle coatings.…”

Section: Discussionmentioning

confidence: 42%

“…The SPIO nanoparticles used in this study were synthesized by classic chemical co-precipitation methods as described previously. 31 Briefly, an aqueous solution (10 mL) of polyglucose sorbitol carboxymethyl ether (PSC, 200 mg) was aerated with nitrogen for 5 min to remove oxygen. Next, 30 mg FeCl 2 (0.236 mmol) and 60 mg FeCl 3 (0.37 mmol) were completely dissolved in 15 mL deionized water, and the mixture was added to the PSC solution.…”

Section: Methodsmentioning

confidence: 99%

Neurite Extension and Orientation of Spiral Ganglion Neurons Can Be Directed by Superparamagnetic Iron Oxide Nanoparticles in a Magnetic Field

Hu¹,

Li²,

Wu³

et al. 2021

IJN

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Introduction Neuroregeneration is a major challenge in neuroscience for treating degenerative diseases and for repairing injured nerves. Numerous studies have shown the importance of physical stimulation for neuronal growth and development, and here we report an approach for the physical guidance of neuron orientation and neurite growth using superparamagnetic iron oxide (SPIO) nanoparticles and magnetic fields (MFs). Methods SPIO nanoparticles were synthesized by classic chemical co-precipitation methods and then characterized by transmission electron microscope, dynamic light scattering, and vibrating sample magnetometer. The cytotoxicity of the prepared SPIO nanoparticles and MF was determined using CCK-8 assay and LIVE/DEAD assay. The immunofluorescence images were captured by a laser scanning confocal microscopy. Cell migration was evaluated using the wound healing assay. Results The prepared SPIO nanoparticles showed a narrow size distribution, low cytotoxicity, and superparamagnetism. SPIO nanoparticles coated with poly-L-lysine could be internalized by spiral ganglion neurons (SGNs) and showed no cytotoxicity at concentrations less than 300 µg/mL. The neurite extension of SGNs was promoted after internalizing SPIO nanoparticles with or without an external MF, and this might be due to the promotion of growth cone development. It was also confirmed that SPIO can regulate cell migration and can direct neurite outgrowth in SGNs preferentially along the direction imposed by an external MF. Conclusion Our results provide a fundamental understanding of the regulation of cell behaviors under physical cues and suggest alternative treatments for sensorineural hearing loss caused by the degeneration of SGNs.

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“…[1][2][3] Recently, there is accumulating evidence that nanomaterials can modulate fate of cells and interactions between nanomaterials and biological cells have attracted considerable attention in facilitating stem cell therapy and bone tissue engineering. [4][5][6] Nanomaterials can flexibly and controllably manipulate biological process of cells to differentiate toward desired lineages. Particularly, nanomaterials with various composition, size, shape or surface chemistry have been widely used as promising osteogenic agents in accelerating osteogenic differentiation of biological cells and bone tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

Citrate-Stabilized Gold Nanorods-Directed Osteogenic Differentiation of Multiple Cells

Zhang¹,

Li²,

Liao³

et al. 2021

IJN

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Gold nanorods (AuNRs) show great potential for versatile biomedical applications, such as stem cell therapy and bone tissue engineering. However, as an indispensable shape-directing agent for the growth of AuNRs, cetyltrimethylammonium bromide (CTAB) is not optimal for biological studies because it forms a cytotoxic bilayer on the AuNR surface, which interferes with the interactions with biological cells. Methods: Citrate-stabilized AuNRs with various aspect-ratios (Cit-NRI, Cit-NRII, and Cit-NRIII) were prepared by the combination of end-selective etching and poly(sodium 4-styrenesulfonate)-assisted ligand exchange method. Their effects on osteogenic differentiation of the pre-osteoblastic cell line (MC3T3-E1), rat bone marrow mesenchymal stem cells (rBMSCs), and human periodontal ligament progenitor cells (PDLPs) have been investigated. Potential signaling pathway of citrate-stabilized AuNRs-induced osteogenic effects was also investigated. Results: The experimental results showed that citrate-stabilized AuNRs have superior biocompatibility and undergo aspect-ratio-dependent osteogenic differentiation via expression of osteogenic marker genes, alkaline phosphatase (ALP) activity and formation of mineralized nodule. Furthermore, Wnt/β-catenin signaling pathway might provide a potential explanation for the citrate-stabilized AuNRs-mediated osteogenic differentiation. Conclusion:These findings revealed that citrate-stabilized AuNRs with great biocompatibility could regulate the osteogenic differentiation of multiple cell types through Wnt/βcatenin signaling pathway, which promote innovative AuNRs in the field of tissue engineering and other biomedical applications.

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Entry-Prohibited Effect of kHz Pulsed Magnetic Field Upon Interaction Between SPIO Nanoparticles and Mesenchymal Stem Cells

Cited by 15 publications

References 35 publications

Superparamagnetic Iron Oxide Nanoparticles Protect Human Gingival Fibroblasts from Porphyromonas gingivalis Invasion and Inflammatory Stimulation

Superparamagnetic Iron Oxide Nanoparticles Protect Human Gingival Fibroblasts from Porphyromonas gingivalis Invasion and Inflammatory Stimulation

Neurite Extension and Orientation of Spiral Ganglion Neurons Can Be Directed by Superparamagnetic Iron Oxide Nanoparticles in a Magnetic Field

Citrate-Stabilized Gold Nanorods-Directed Osteogenic Differentiation of Multiple Cells

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