Entrenamiento microquirúrgico básico para realizar un modelo animal de alotrasplante compuesto vascularizado

Abstract

Basic microsurgical training for a vascularized composite allotransplantation animal model Introduction: Rat hind limb transplantation is a complex animal model of vascularized composite allotransplantation (VCA). A basic microsurgical training is required prior to the implementation of this model. Aim: To propose a training program for the acquisition of basic skills to perform a microsurgical VCA model. Animals and Methods: The training program was conducted in two stages. First, at the dry lab, basic suturing skills with 9-0 to 11-0 nylon sutures were practiced, reproduced from surgical videos performed by experts. In a second stage, at the wet lab using 13 Lewis rats, 6 hind limb microsurgical dissections were performed and the important steps for transplantation were identified: 10 end to end femoral artery anastomoses with 10-0 nylon interrupted suture; 10 end to end femoral vein anastomoses with 10-0 nylon suture; 3 femoral vein interposition in the femoral artery; 6 end to end sciatic nerve neurorrhaphy; 4 femur osteosynthesis with 21g needle and wire cerclage. Anastomotic patency rate and anastomotic surgical time were recorded. Results: Arterial and venous patency rate was 100 and 90% respectively. Surgical time decreased from 49 to 24 minutes on arterial anastomoses and from 55 to 25 minutes on venous anastomoses after completion

Evaluación de la técnica quirúrgica “penopexia perineal” en la preparación de toros marcadores para la empresa Bovina

“…La técnica quirúrgica penopexia perineal demostró una satisfacción aceptable para el productor, debido al tiempo rápido de recuperación de los toretes y una relación costo-beneficio accesible, cuadro 5. Los resultados comparando la posición durante la intervención, figura 6 mostraron que el tiempo promedio total fue menor en estación p<0.05, basado en los procesos quirúrgicos la cuestión del manejo demuestra una manera eficiente del trato del paciente con los objetivos de una mayor rapidez en la recuperación fisiológica e incorporación a su función zootécnica (Ramírez M et al, 2013). Los toros celadores optimizan un manejo reproductivo en un programa de inseminación artificial, la recuperación posoperatoria es prioritaria dentro la función zootécnica, desempeñando mejor recuperación, la técnica es mínima invasiva y se suma a una opción más para la preparación de toros celadores.…”

Dye-Perfused Human Placenta for Simulation in a Microsurgery Laboratory for Plastic Surgeons

“…Currently, the rat is the standard model for microsurgical training due to its greater fidelity, the biomechanical qualities of the blood vessel in operating rooms as well as the possibility it offers of a better evaluation of anastomotic patency. 13 Although human placenta is a low-cost, available ex vivo simulation model that provides an alternative to the use of live animals; considering the abundant vasculature of the human placenta this model allows extended practice for the refinement of the microsurgical skills.…”

An innovative Ear Transplantation for Vascularized Composite Allotransplantation Research in Porcine Model

“…This similarity is critical considering the complexity of these procedures. Using this approach to develop training guidelines could considerably simplify transplant procedures, contributing to the current transplant protocols and potentially enhancing the tolerance and survival rates of VCA procedures (45)(46)(47)(48)(49)(50). However, VCA loss remains a risk due to factors such as rejection, surgical errors, vascular occlusion from thrombi, anastomosis dehiscence, local trauma affecting circulation, and compatibility issues between the biomodels (17,19,51) Advances in the surgical method of neck dissection have resulted in modi cations that improve the procedure's simplicity.…”