Entrapment of organic fluorophores in calcium phosphate nanoparticles with slow release

Sadallah, Laila; Boukhris, Aicha; Hannache, Hassan; Gmouh, Saïd

doi:10.3906/kim-1902-57

Cited by 3 publications

(1 citation statement)

References 40 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…30 ) added outside. By adsorbing fluorescein to the mineral 85 , the resulting particles could be imaged (Fig. 5d and Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Artificial cell synthesis using biocatalytic polymerization-induced self-assembly

Belluati,

Jimaja,

Chadwick

et al. 2023

Nat. Chem.

View full text Add to dashboard Cite

Artificial cells are biomimetic microstructures that mimic functions of natural cells, can be applied as building blocks for molecular systems engineering, and host synthetic biology pathways. Here we report enzymatically synthesized polymer-based artificial cells with the ability to express proteins. Artificial cells were synthesized using biocatalytic atom transfer radical polymerization-induced self-assembly, in which myoglobin synthesizes amphiphilic block co-polymers that self-assemble into structures such as micelles, worm-like micelles, polymersomes and giant unilamellar vesicles (GUVs). The GUVs encapsulate cargo during the polymerization, including enzymes, nanoparticles, microparticles, plasmids and cell lysate. The resulting artificial cells act as microreactors for enzymatic reactions and for osteoblast-inspired biomineralization. Moreover, they can express proteins such as a fluorescent protein and actin when fed with amino acids. Actin polymerizes in the vesicles and alters the artificial cells’ internal structure by creating internal compartments. Thus, biocatalytic atom transfer radical polymerization-induced self-assembly-derived GUVs can mimic bacteria as they are composed of a microscopic reaction compartment that contains genetic information for protein expression upon induction.

show abstract

“…30 ) added outside. By adsorbing fluorescein to the mineral 85 , the resulting particles could be imaged (Fig. 5d and Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Artificial cell synthesis using biocatalytic polymerization-induced self-assembly

Belluati,

Jimaja,

Chadwick

et al. 2023

Nat. Chem.

View full text Add to dashboard Cite

show abstract

Deposition of Phosphate Nanoparticles onto Textile Fabrics via Sol-gel Method and Their Kinetics Desorption Studies

messoudi

Boukhriss

Sadallah

et al. 2020

Chem. Res. Chin. Univ.

View full text Add to dashboard Cite

Synthesis of artificial cells via biocatalytic polymerisation-induced self-assembly

Jimaja

Chadwick

Glynn

et al. 2023

Preprint

View full text Add to dashboard Cite

Artificial cells are biomimetic microstructures that mimic functions of natural cells and find application, e.g., as microreactors, as building blocks for molecular systems engineering, and to host synthetic biology pathways. Here, we report enzymatically synthesised polymer-based artificial cells with the ability to express proteins. They are created by biocatalytic atom transfer radical polymerization-induced self-assembly (bioPISA). The metalloprotein myoglobin synthesises amphiphilic block copolymers that self-assemble into structures ranging from micelles over worm-like micelles to polymersomes and giant unilamellar vesicles (GUVs). The GUVs encapsulate cargo during the polymerisation, including enzymes, nanoparticles, microparticles, plasmids and cell lysate. The resulting artificial cells act as microreactors for enzymatic reactions and for osteoblast-inspired biomineralization, and could express proteins when fed with amino acids, as shown by the expression of the fluorescent protein mClover and of actin. Actin polymerises in the vesicles and alters the artificial cell’s internal structure by creating internal compartments. Thus, bioPISA-derived GUVs mimic bacteria as they are composed of a microscopic reaction compartment that contains genetic information which is able to express proteins upon induction. bioPISA not only is a powerful tool in the pursuit of artificial cells but also for the easy and highly efficient encapsulation of biological molecules under mild conditions and in biologically relevant media. Therefore, it could have significant implications for the development of biomaterials and drug-delivery systems, as well as for cell encapsulation, and the in-situ formation of nano-objects.

show abstract

Entrapment of organic fluorophores in calcium phosphate nanoparticles with slow release

Cited by 3 publications

References 40 publications

Artificial cell synthesis using biocatalytic polymerization-induced self-assembly

Artificial cell synthesis using biocatalytic polymerization-induced self-assembly

Deposition of Phosphate Nanoparticles onto Textile Fabrics via Sol-gel Method and Their Kinetics Desorption Studies

Synthesis of artificial cells via biocatalytic polymerisation-induced self-assembly

Contact Info

Product

Resources

About