Entrapment of membrane proteins in sol-gel derived silica

Critical Effect of Polyelectrolytes on the Electrochemical Response of Dehydrogenases Entrapped in Sol‐Gel Thin Films

“…Since the discovery that alkaline phosphatase can be entrapped in a sol-gel-derived silica matrix while maintaining its biological activity [11] and that the stability of biomolecules can even be improved upon entrapment [12], sol-gel bioencapuslation has become an intense research area [13][14][15][16]. The process typically involves the hydrolysis of tetraalkoxysilane in acidic medium followed by the addition of biomolecules in the presence of a buffer with a pH of about 7, which initiates condensation of the hydrolyzed precursors around the biomolecules that become physically entrapped in an active form within the porous framework.…”

“…The sol can be prepared by using another silica source, for example a sodium silicate solution [20], or a mixture of sodium silicate and Ludox suspension [21,22]. Recent efforts have been made to optimize the process by controlling the porosity of bioencapsulates and the chemical environment of trapped species This notably involved the use of biocompatible silane precursors, protein-stabilizing additives, charged polymers, or sugars and aminopeptides [15,16,23].…”

Overview of the main methods used to combine proteins with nanosystems: absorption, bioconjugation, and encapsulation

“…139 Silica is indeed considered a very appealing material for drug delivery systems because it is relatively inexpensive, chemically inert, thermally stable, and biocompatible. Amorphous silica, used for decades as a food additive and for specific applications, is generally regarded as safe.…”

“…In this approach, polypeptides, especially enzymes, could be entrapped inside silica matrix allowing the retention of enzymatic activity. [139][140][141] On the other hand, process difficulties such as uncontrolled release, denaturation and the hardness control of the protein Nanotechnology methods used to combine proteins Dovepress submit your manuscript | www.dovepress.com Dovepress orientation can be found. 142,143 The control of the drug release of such silica nanoparticles is the most important and difficult parameter that needs to be properly tuned.…”

Development and characterisation of integrated microfluidics on waveguide-based photonic platforms fabricated from hybrid materials

“…This synthesis route affords a great deal of control over the material composition via the correct choice of chemical precursors and synthesis conditions, resulting in a class of materials that are highly tunable. Multiple functionalities are often incorporated in a single hybrid material, which can serve the needs of diverse applicationsranging from electrochromic coatings for windows (Avellaneda et al 2002;Avendano et al 2006;Karuppasamy and Subrahmanyam 2007), corrosion protection (Varma et al 2010;Hamdy 2006), porous sensor membranes for gas phase sensing (Higgins et al 2008; Lei et al 2006), entrapment of biomolecular probes for biosensors (Besanger and Brennan 2006;Gupta and Chaudhury 2007). Compared to pure organic polymers, sol-gel processed hybrid materials have advantages linked not only to their superior tunability as outlined above, but also linked to the production of interpenetrating, covalently bonded organic and inorganic networks within a single material, permitting the synthesis of materials with unique combinations of properties.…”