Entrapment of live microbial cells in electropolymerized polyaniline and their use as urea biosensor

Biosensors on Enzymes, Tissues, and Cells

“…In 2009 Jha et al 35 reported the use of polystyrene sulfonate-polyaniline (PSS-PANI) conducting polymer to immobilize lyophilized Brevibacterium ammoniagenes cells on platinum wire electrodes to detect urea and achieve a response time of 3 min. Furthermore, they used potentiostatic electropolymerization and suggested that this is one of the safest techniques for immobilization of live cells because it is not as harsh as other polymerization methods.…”

Immobilisation and Biosensors

“…A platinum interdigitated electrode was used to immobilise C. vulgaris using a bovine serum albumin membrane (Chouteau et al 2005 ). Brevibacterium ammoniagenes was used in the development of a conductometric biosensor by immobilising the lyophilised culture in pH-sensitive polyaniline on a Pt twin wire electrode to detect urea (Jha et al 2009 ).…”

Urea Biosensor Based on Conducting Polymer Transducers

“…al. [25] used electropolymerized polyaniline-polystyrene (PANI-PSS) with brevibacterium ammoginase as a biocatalyst for urea biosensor. The immobilized cell works as a source of unpurified urease to develop conductometrc urea biosensor.…”