Entomomonas moraniae gen. nov., sp. nov., a member of the family Pseudomonadaceae isolated from Asian honey bee gut, possesses a highly reduced genome

Wang, Jieni; Su, Qinzhi; Zhang, Xue; Li, Chenyi; Luo, Shiqi; Zhou, Xin; Zheng, Hao

doi:10.1099/ijsem.0.003731

Cited by 18 publications

(16 citation statements)

References 46 publications

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“…These characteristics were also reported for E. moraniae QZS01 T [1]. The most striking difference in the two fatty acid profiles was the significant amount of C 15 : 0 , which is absent in E.…”

Section: Full-textsupporting

confidence: 77%

“…The presence of the unidentified lipids GL1, GL2, PL2, PL3 and APL were not reported for E. moraniae QZS01 T [1].…”

Section: Full-textmentioning

confidence: 99%

“…On the other hand, Q-8 is a characteristic of E. moraniae QZS01 T [1]. The polar lipid profile of strain F2A T () consisted of the major lipids diphosphatidylglycerol and phosphatidylethanolamine, moderate amounts of phosphatidylglycerol and an unidentified phospholipid (PL1), and minor amounts of two unidentified phospholipids (PL2, PL3), an unidentified aminophospholipid (APL), and two unidentified glycolipids (GL1, GL2).…”

Section: Full-textmentioning

confidence: 99%

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Entomomonas asaccharolytica sp. nov., isolated from Acheta domesticus

Busse¹,

Kämpfer

Szostak³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization. Cells of the strain were rod-shaped, Gram-stain-negative and catalase- and oxidase-positive. It did not assimilate any carbohydrates. The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %). The next highest similarity values were found to representatives of related genera (<93 %). The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%. Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively. The quinone system predominantly contained ubiquinone Q-8. In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected. The polyamine pattern consisted of the major compounds putrescine and spermidine. Major fatty acids were C18 : 1 ω7c and C16 : 0 and the hydroxyl acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp. nov. F2AT (=CCM 9136T=LMG 32211T).

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Section: Full-textsupporting

confidence: 77%

“…The presence of the unidentified lipids GL1, GL2, PL2, PL3 and APL were not reported for E. moraniae QZS01 T [1].…”

Section: Full-textmentioning

confidence: 99%

Section: Full-textmentioning

confidence: 99%

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Entomomonas asaccharolytica sp. nov., isolated from Acheta domesticus

Busse¹,

Kämpfer

Szostak³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…While not highly host-specific, at least some of the dominant bacteria in butterflies appear to be insect specialists as opposed to cosmopolitan environmental taxa. For example, phylogenetic evidence supports a general insect association for Entomomonas (38), Apibacter (70), and Orbus (Fig. 5) (71).…”

Section: Discussionmentioning

confidence: 85%

“…Another case of ambiguous taxonomy occurred with sequences classified as Pseudomonas by the SILVA reference database, which we label below as "Entomomonas/Pseudomonas". Entomomonas is a recently described genus of apparently insect-specialized Pseudomonadaceae and includes several sequences retrieved from Heliconius genome assemblies (38).…”

Section: Amplicon Data Processingmentioning

confidence: 99%

Heliconiusbutterflies host characteristic and phylogenetically structured adult-stage microbiomes

Hammer

Dickerson

McMillan

et al. 2019

Preprint

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1 2 Evolutionary transitions in animal diets often coincide with shifts in the microbiome, but 3 the degree to which diet-microbiome feedbacks vary across host taxa and development is 4 unresolved. We examined these potential feedbacks from the perspective of Lepidoptera 5 (butterflies and moths), a diverse clade in which little is known about adult-stage microbial 6 associations. With the exception of Heliconius butterflies, most lepidopteran adults are short-7 lived and either feed on simple substrates, like nectar, or do not feed at all. Heliconius consume 8 pollen as adults, which provides amino acids and allows the butterflies to have an extended 9 lifespan. Using 16S rRNA gene sequencing of 214 field-collected individuals, we found that 10 adult passion-vine butterfly microbiomes exhibited a strong signal of host phylogeny, with a 11 clear distinction between Heliconius and non-pollen-feeding relatives. This pattern was largely 12 driven by differing relative abundances of bacterial phylotypes shared among host taxa, as 13 opposed to the presence or absence of host-specific phylotypes. Using shotgun metagenomic 14 sequencing, we also discovered trypanosomatids and microsporidia to be prevalent in butterfly 15 guts, suggesting potential interactions with co-localized gut bacteria. Overall, we show that a 16 major transition in adult-stage lepidopteran diet and life history coincides with a shift in 17 microbiomes, and our work provides a foundation for future tests of microbiome function in 18 adult butterflies. 19 20 Lepidoptera (butterflies and moths) are a diverse and ecologically important group in 31 which microbiome roles are likely to be highly specific to certain host taxa and life stages. For 32 lepidopteran larvae (caterpillars), even major shifts in larval diet do not coincide with 33 functionally relevant shifts in the microbiome [9][10][11]. Lepidopteran larvae typically harbor a 34 very low number of gut microbes, and most of these microbes are transient with the exception of 35 pathogen infections [9]. However, the microbiomes of insects that undergo complete 36 metamorphosis, such as Lepidoptera, often exhibit profound shifts in size, composition and 37 function across host life stages [7]. A recent survey of various butterfly species found that adult-38 stage gut bacterial communities were generally abundant (median ~10 8 16S rRNA gene copies 39 per gut) and distinct from diet-associated bacterial communities [12]. It is possible that 40 evolutionary transitions in the diet of adult lepidopterans could cause, or be caused by, 41 concomitant transitions in adult-stage microbiomes. 42Arguably, the single most dramatic and consequential transition in adult diet among the 43 150,000+ described species of Lepidoptera [13] occurred in the ancestors of neotropical 44Heliconius butterflies (Nymphalidae: Heliconiini). While adults of other lepidopterans either do 45 not feed at all, or feed on comparatively nitrogen-poor and/or inconsistently available substrates 46 [14], Heliconius evolved the abili...

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