Entomomonas asaccharolytica sp. nov., isolated from Acheta domesticus

Abstract

Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization. Cells of the strain were rod-shaped, Gram-stain-negative and catalase- and oxidase-positive. It did not assimilate any carbohydrates. The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %). The next highest similarity values were found to representatives of related genera (<93 %). The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%. Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively. The quinone system predominantly contained ubiquinone Q-8. In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected. The polyamine pattern consisted of the major compounds putrescine and spermidine. Major fatty acids were C18 : 1 ω7c and C16 : 0 and the hydroxyl acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp. nov. F2AT (=CCM 9136T=LMG 32211T).

Complete Genome Sequence of Entomomonas sp. Strain E2T0, Isolated from the Darkling Beetle Zophobas morio Larvae

“…Members of the genus Entomomonas are associated with the digestive tract of insects ( 1 ). To date, only two Entomomonas spp.…”

“…To date, only two Entomomonas spp. have been sequenced ( 1 , 2 ). The antibiotic resistance profiles of these two strains have not been determined, but both strains possessed uncharacterized β-lactamase genes.…”

Sagittula salina sp. nov., isolated from marine waste

“…The phenotypic characterization of strain M10.9X T was carried out after a week of growth at 30 °C. The Gram-staining test was performed with KOH 3 % (w/v), recording viscosity as a positive result for Gram-negative bacteria [5, 6]. In order to test oxidase activity, a commercial oxidase test stick for microbiology (PanReac AppliChem) was used following the manufacturer’s instructions.…”